PMID- 30802749
OWN - NLM
STAT- MEDLINE
DCOM- 20190729
LR  - 20190729
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 168
DP  - 2019 May 10
TI  - Rapid identification of triptolide in Tripterygium wilfordii products by gold 
      immunochromatographic assay.
PG  - 102-112
LID - S0731-7085(18)32629-3 [pii]
LID - 10.1016/j.jpba.2019.02.020 [doi]
AB  - Triptolide colloidal gold immunoassay strip was prepared by using colloidal gold 
      labeled monoclonal antibody purified with saturated ammonium sulfate, triptolide 
      succinate-bovine serum albumin and sheep anti-mouse IgG for the first time in our 
      lab. Under optimized conditions, the detection limit of TP strip prepared in this 
      study were determined to be 1 mug/mL and the total analysis time (including sample 
      preparation) was less than 50 min. Sixty-six batches of Tripterygium wilfordii 
      (T. wilfordii) and its adulterations were analyzed. It was found that 28 batches 
      of T. wilfordii were positive as detected by the strips and 38 batches were 
      negative. The result of test strip detection was in consistent with that from the 
      UFLC-ESI-MS/MS method. Gold immunochromatography assay was demonstrated as a 
      rapid, cost-effective, reliable technique for on-site screening of T. wilfordii 
      and its preparations. The prepared colloidal gold immunoassay strip can be 
      conveniently used for rapid identification of triptolide containing products.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Duan, Yaping
AU  - Duan Y
AD  - College of Traditional Chinese Medicine, Jilin Agricultural University, 
      Changchun, 130118, China; Key Laboratory of Bioactive Substances and Resources 
      Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of 
      Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union 
      Medical College, Beijing, 100193, China.
FAU - Luo, Jiaoyang
AU  - Luo J
AD  - Key Laboratory of Bioactive Substances and Resources Utilization of Chinese 
      Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, 
      Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 
      100193, China.
FAU - Liu, Congmin
AU  - Liu C
AD  - Key Laboratory of Bioactive Substances and Resources Utilization of Chinese 
      Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, 
      Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 
      100193, China.
FAU - Shan, Linan
AU  - Shan L
AD  - College of Traditional Chinese Medicine, Jilin Agricultural University, 
      Changchun, 130118, China; Key Laboratory of Bioactive Substances and Resources 
      Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of 
      Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union 
      Medical College, Beijing, 100193, China.
FAU - Dou, Xiaowen
AU  - Dou X
AD  - Key Laboratory of Bioactive Substances and Resources Utilization of Chinese 
      Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, 
      Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 
      100193, China.
FAU - Yang, Shihai
AU  - Yang S
AD  - College of Traditional Chinese Medicine, Jilin Agricultural University, 
      Changchun, 130118, China. Electronic address: jlyangs@163.com.
FAU - Yang, Meihua
AU  - Yang M
AD  - Key Laboratory of Bioactive Substances and Resources Utilization of Chinese 
      Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, 
      Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 
      100193, China. Electronic address: yangmeihua15@hotmail.com.
LA  - eng
PT  - Journal Article
DEP - 20190214
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Diterpenes)
RN  - 0 (Epoxy Compounds)
RN  - 0 (Gold Colloid)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Phenanthrenes)
RN  - 0 (RG 490-88)
RN  - 0 (Reagent Strips)
RN  - 19ALD1S53J (triptolide)
RN  - 27432CM55Q (Serum Albumin, Bovine)
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - Cattle
MH  - Diterpenes/*analysis/chemistry
MH  - Epoxy Compounds/analysis
MH  - Gold Colloid/chemistry
MH  - Immunoassay/*methods
MH  - Immunoglobulin G/immunology
MH  - Limit of Detection
MH  - Phenanthrenes/*analysis
MH  - Reagent Strips
MH  - Serum Albumin, Bovine
MH  - Sheep
MH  - Tandem Mass Spectrometry/methods
MH  - Tripterygium/*chemistry
OTO - NOTNLM
OT  - Gold nanoparticle immunochromatographic strip
OT  - Quality control
OT  - Tripterygium wilfordii
OT  - Triptolide
OT  - UFLC-ESI-MS/MS
EDAT- 2019/02/26 06:00
MHDA- 2019/07/30 06:00
CRDT- 2019/02/26 06:00
PHST- 2018/11/23 00:00 [received]
PHST- 2019/02/12 00:00 [revised]
PHST- 2019/02/13 00:00 [accepted]
PHST- 2019/02/26 06:00 [pubmed]
PHST- 2019/07/30 06:00 [medline]
PHST- 2019/02/26 06:00 [entrez]
AID - S0731-7085(18)32629-3 [pii]
AID - 10.1016/j.jpba.2019.02.020 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2019 May 10;168:102-112. doi: 10.1016/j.jpba.2019.02.020. 
      Epub 2019 Feb 14.