PMID- 30803307
OWN - NLM
STAT- MEDLINE
DCOM- 20200519
LR  - 20200519
IS  - 1552-4531 (Electronic)
IS  - 0748-7304 (Linking)
VI  - 34
IP  - 2
DP  - 2019 Apr
TI  - Achilles-Mediated and Sex-Specific Regulation of Circadian mRNA Rhythms in 
      Drosophila.
PG  - 131-143
LID - 10.1177/0748730419830845 [doi]
AB  - The circadian clock is an evolutionarily conserved mechanism that generates the 
      rhythmic expression of downstream genes. The core circadian clock drives the 
      expression of clock-controlled genes, which in turn play critical roles in 
      carrying out many rhythmic physiological processes. Nevertheless, the molecular 
      mechanisms by which clock output genes orchestrate rhythmic signals from the 
      brain to peripheral tissues are largely unknown. Here we explored the role of one 
      rhythmic gene, Achilles, in regulating the rhythmic transcriptome in the fly 
      head. Achilles is a clock-controlled gene in Drosophila that encodes a putative 
      RNA-binding protein. Achilles expression is found in neurons throughout the fly 
      brain using fluorescence in situ hybridization (FISH), and legacy data suggest it 
      is not expressed in core clock neurons. Together, these observations argue 
      against a role for Achilles in regulating the core clock. To assess its impact on 
      circadian mRNA rhythms, we performed RNA sequencing (RNAseq) to compare the 
      rhythmic transcriptomes of control flies and those with diminished Achilles 
      expression in all neurons. Consistent with previous studies, we observe dramatic 
      upregulation of immune response genes upon knock-down of Achilles. Furthermore, 
      many circadian mRNAs lose their rhythmicity in Achilles knock-down flies, 
      suggesting that a subset of the rhythmic transcriptome is regulated either 
      directly or indirectly by Achilles. These Achilles-mediated rhythms are observed 
      in genes involved in immune function and in neuronal signaling, including Prosap, 
      Nemy and Jhl-21. A comparison of RNAseq data from control flies reveals that only 
      42.7% of clock-controlled genes in the fly brain are rhythmic in both males and 
      females. As mRNA rhythms of core clock genes are largely invariant between the 
      sexes, this observation suggests that sex-specific mechanisms are an important, 
      and heretofore under-appreciated, regulator of the rhythmic transcriptome.
FAU - Li, Jiajia
AU  - Li J
AD  - Division of Pulmonary and Critical Care Medicine, Washington University School of 
      Medicine, St. Louis, MO, USA.
FAU - Yu, Renee Yin
AU  - Yu RY
AD  - Centre for Research in Neuroscience, Research Institute of the McGill University 
      Health Centre, Montreal General Hospital, Montreal, Quebec, Canada.
FAU - Emran, Farida
AU  - Emran F
AD  - Centre for Research in Neuroscience, Research Institute of the McGill University 
      Health Centre, Montreal General Hospital, Montreal, Quebec, Canada.
FAU - Chen, Brian E
AU  - Chen BE
AD  - Centre for Research in Neuroscience, Research Institute of the McGill University 
      Health Centre, Montreal General Hospital, Montreal, Quebec, Canada.
AD  - Departments of Medicine and Neurology and Neurosurgery, McGill University, 
      Montreal, Quebec, Canada.
FAU - Hughes, Michael E
AU  - Hughes ME
AD  - Division of Pulmonary and Critical Care Medicine, Washington University School of 
      Medicine, St. Louis, MO, USA.
LA  - eng
GR  - R21 AR069266/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20190225
PL  - United States
TA  - J Biol Rhythms
JT  - Journal of biological rhythms
JID - 8700115
RN  - 0 (Drosophila Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
SB  - IM
MH  - Animals
MH  - Circadian Clocks/genetics
MH  - Circadian Rhythm/*genetics
MH  - Drosophila/*genetics/physiology
MH  - Drosophila Proteins/*genetics
MH  - Female
MH  - *Gene Expression Regulation
MH  - Male
MH  - Neurons/physiology
MH  - RNA, Messenger/*genetics
MH  - RNA-Binding Proteins/genetics
MH  - Sequence Analysis, RNA
MH  - *Sex Factors
MH  - Transcriptome
MH  - Up-Regulation
OTO - NOTNLM
OT  - RNA sequencing (RNAseq)
OT  - circadian rhythms
OT  - clock-controlled genes (CCGs)
OT  - sex specificity
EDAT- 2019/02/26 06:00
MHDA- 2020/05/20 06:00
CRDT- 2019/02/27 06:00
PHST- 2019/02/26 06:00 [pubmed]
PHST- 2020/05/20 06:00 [medline]
PHST- 2019/02/27 06:00 [entrez]
AID - 10.1177/0748730419830845 [doi]
PST - ppublish
SO  - J Biol Rhythms. 2019 Apr;34(2):131-143. doi: 10.1177/0748730419830845. Epub 2019 
      Feb 25.