PMID- 30804810
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 10
DP  - 2019
TI  - Effects of Fungicide Propiconazole on the Yeast-Like Symbiotes in Brown 
      Planthopper (BPH, Nilaparvata lugens Stal) and Its Role in Controlling BPH 
      Infestation.
PG  - 89
LID - 10.3389/fphys.2019.00089 [doi]
LID - 89
AB  - Yeast-like symbiotes (YLS), harbored in the abdomen fat-body cells of the rice 
      brown planthopper (BPH), Nilaparvata lugens Stal (Hemiptera: Delphacidae), are 
      vital to the growth and reproduction of their host. It is feasible to manipulate 
      BPH infestation on rice by inhibiting YLS using fungicide. In this study, the 
      fungicide propiconazole was injected into the hemolymph of BPH thorax via 
      microinjection to investigate its effect on YLS, especially the dominant species, 
      Hypomyces chrysospermus, and their host BPH. Propiconazole markedly reduced the 
      total number of YLS and H. chrysospermus in BPH hemolymph and fat body, thereby 
      leading to an obvious higher mortality and lower fecundity of BPH than the 
      negative control (PBS, phosphate buffer solution). After microinjecting 
      propiconazole, the survival rate of BPH nymphs at the 5th instar was 
      significantly lower than that obtained after PBS treatment. Eight days after 
      propiconazole microinjection, the BPH survival rate dropped to 40%, only half of 
      BPH survival rate treated with PBS microinjection. For female adults (1-day-old), 
      there were significant differences in the survival rates between BPHs treated 
      with propiconazole and those treated with PBS at days 5-8. The fecundity of BPH 
      decreased significantly by microinjecting propiconazole and averaged only 229 
      eggs per female, which was 20% less than that of the negative control. 
      Furthermore, we reared BPH on the susceptible variety TN1 sprayed with 
      propiconazole to prove the feasibility manipulating field occurrence of BPH by 
      inhibiting YLS using fungicides. The number of YLS and H. chrysospermus in BPH 
      obviously declined. Subsequently, the survival rate and fecundity of BPH 
      significantly decreased after feeding on rice treated with propiconazole. 
      Meanwhile, the propiconazole residue was detected in the hemolymph and gut of BPH 
      by HPLC analysis within 1 day of feeding. Inhibiting YLS using fungicides was a 
      novel and effective way to control BPH infestation.
FAU - Shentu, Xuping
AU  - Shentu X
AD  - Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, 
      College of Life Sciences, China Jiliang University, Hangzhou, China.
FAU - Wang, Xiaolong
AU  - Wang X
AD  - Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, 
      College of Life Sciences, China Jiliang University, Hangzhou, China.
FAU - Xiao, Yin
AU  - Xiao Y
AD  - Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, 
      College of Life Sciences, China Jiliang University, Hangzhou, China.
FAU - Yu, Xiaoping
AU  - Yu X
AD  - Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, 
      College of Life Sciences, China Jiliang University, Hangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20190211
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
PMC - PMC6378626
OTO - NOTNLM
OT  - Hypomyces chrysospermus
OT  - Nilaparvata lugens
OT  - microinjection
OT  - propiconazole
OT  - yeast-like symbiotes
EDAT- 2019/02/26 06:00
MHDA- 2019/02/26 06:01
PMCR- 2019/02/11
CRDT- 2019/02/27 06:00
PHST- 2018/07/28 00:00 [received]
PHST- 2019/01/25 00:00 [accepted]
PHST- 2019/02/27 06:00 [entrez]
PHST- 2019/02/26 06:00 [pubmed]
PHST- 2019/02/26 06:01 [medline]
PHST- 2019/02/11 00:00 [pmc-release]
AID - 10.3389/fphys.2019.00089 [doi]
PST - epublish
SO  - Front Physiol. 2019 Feb 11;10:89. doi: 10.3389/fphys.2019.00089. eCollection 
      2019.