PMID- 30805994
OWN - NLM
STAT- MEDLINE
DCOM- 20200827
LR  - 20200827
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Print)
IS  - 0730-2312 (Linking)
VI  - 120
IP  - 8
DP  - 2019 Aug
TI  - Macrophage colony-stimulating factor pretreatment of bone marrow progenitor cells 
      regulates osteoclast differentiation based upon the stage of myeloid development.
PG  - 12450-12460
LID - 10.1002/jcb.28512 [doi]
AB  - Osteoclasts (OCs) are large, multinucleated bone resorbing cells originating from 
      the bone marrow myeloid lineage, and share a common progenitor with macrophages 
      and dendritic cells. Bone marrow cells (BMCs) are a common source for in vitro 
      osteoclastogenesis assays but are a highly heterogeneous mixture of cells. 
      Protocols for in vitro osteoclastogenesis vary considerably thus hindering 
      interpretation and comparison of results between studies. Macrophage 
      colony-stimulating factor (M-CSF) pretreatment is commonly used to expand OC 
      progenitors (OCPs) in BMC cultures before in vitro differentiation. However, the 
      failure of osteoclastogenesis of M-CSF primed bone marrow myeloid blasts has been 
      reported. In this study, we used a simple method of differential adherence to 
      plastic to enrich OCP from mouse BMCs. We found that M-CSF pretreatment of 
      plastic-adherent BMCs (adBMCs) increased the number of CD11b-F4/80+ macrophages 
      and decreased the number of CD11b+ monocytes resulting in decreased OC formation. 
      M-CSF pretreatment of purified c-Kit+ progenitors weakly inhibited OC formation, 
      whereas M-CSF pretreatment of purified c-Kit-CD11b+ progenitors promoted the 
      formation of large OC. M-CSF pretreatment increased the proliferation of both 
      purified c-Kit+ and c-Kit-CD11b+ cells and increased the percentage of 
      CD11b-F4/80+ cells from c-Kit+ progenitors. In addition, M-CSF pretreatment 
      increased the percentage of CD11b+ F4/80- cells from purified c-Kit-CD11b+ cells. 
      M-CSF pretreatment increased the percentage of CD14 + CD16 + intermediate 
      monocytes and subsequent OC formation from human 2adBMCs, and increased OC 
      formation of purified CD14 + cells. Together, these results indicate that in 
      vitro OCP expansion in the presence of M-CSF and bone marrow stromal cells is 
      dependent upon the developmental stage of myeloid cells, in which M-CSF favors 
      macrophage differentiation of multipotent progenitors, promotes monocyte 
      maturation and supports differentiation of late-stage OCP cells.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Yang, Xuehui
AU  - Yang X
AD  - Center for Molecular Medicine, Maine Medical Center Research Institute, 
      Scarborough, Maine.
FAU - Pande, Shivangi
AU  - Pande S
AD  - Center for Molecular Medicine, Maine Medical Center Research Institute, 
      Scarborough, Maine.
AD  - Graduate School of Biomedical Sciences and Engineering, University of Maine, 
      Orono, Maine.
FAU - Scott, Cameron
AU  - Scott C
AD  - Department of Biology, University of Southern Maine, Portland, Maine.
FAU - Friesel, Robert
AU  - Friesel R
AUID- ORCID: 0000-0002-8850-7505
AD  - Center for Molecular Medicine, Maine Medical Center Research Institute, 
      Scarborough, Maine.
AD  - Graduate School of Biomedical Sciences and Engineering, University of Maine, 
      Orono, Maine.
LA  - eng
GR  - P30 GM106391/GM/NIGMS NIH HHS/United States
GR  - U54 GM115516/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20190225
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology/drug effects/metabolism
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Female
MH  - Hematopoiesis
MH  - Macrophage Colony-Stimulating Factor/administration & dosage/*pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Myeloid Cells/*cytology/drug effects/metabolism
MH  - Osteoclasts/*cytology/drug effects/metabolism
MH  - *Osteogenesis
MH  - Stem Cells/*cytology/drug effects/metabolism
PMC - PMC6570541
MID - NIHMS1012897
OTO - NOTNLM
OT  - CD11b
OT  - CD14
OT  - CD16
OT  - c-Kit
OT  - macrophage colony-stimulating factor (M-CSF)
OT  - osteoclast progenitor (OCP)
OT  - osteoclastogenesis
COIS- Conflicts of interest: The authors declare that they have no conflicts of 
      interest.
EDAT- 2019/02/26 06:00
MHDA- 2020/08/28 06:00
PMCR- 2020/08/01
CRDT- 2019/02/27 06:00
PHST- 2018/11/28 00:00 [received]
PHST- 2019/01/11 00:00 [accepted]
PHST- 2019/02/26 06:00 [pubmed]
PHST- 2020/08/28 06:00 [medline]
PHST- 2019/02/27 06:00 [entrez]
PHST- 2020/08/01 00:00 [pmc-release]
AID - 10.1002/jcb.28512 [doi]
PST - ppublish
SO  - J Cell Biochem. 2019 Aug;120(8):12450-12460. doi: 10.1002/jcb.28512. Epub 2019 
      Feb 25.