PMID- 30806059
OWN - NLM
STAT- MEDLINE
DCOM- 20190611
LR  - 20190613
IS  - 1872-2075 (Electronic)
IS  - 1000-3061 (Linking)
VI  - 35
IP  - 2
DP  - 2019 Feb 25
TI  - [Effect of 27nt-miRNA on the differentiation of mesenchymal stem cells into 
      vascular smooth muscle cells].
PG  - 290-297
LID - 10.13345/j.cjb.180243 [doi]
AB  - To investigate the effect of 27nt-miRNA on the differentiation of mesenchymal 
      stem cells into vascular smooth muscle cells. The highly expression plasmids of 
      27nt-miRNA and anti-27nt-miRNA, and negative control plasmids were constructed, 
      packaged with lentivirus and transfected into human umbilical cord mesenchymal 
      stem cells (hUCMSCs). Collagen IV was added to induce hUCMSCs differentiation 
      into blood vessel smooth muscle cells (VSMCs). The cell viability was measured by 
      MTT assay. The expression of SMA, SM22alpha at mRNA and protein levels was determined 
      by RT-PCR, immunocytochemical staining and Western blotting. Compared with the 
      negative control group, the viability of the 27nt-miRNA overexpression group was 
      decreased by 20.48% (P<0.05), and the expression of SMA mRNA and SM22alpha mRNA and 
      protein was significantly increased (P<0.05); the viability of Anti-27nt-miRNA 
      group was increased 18.07% (P<0.05), and the expression of SMA mRNA and SM22alpha 
      mRNA and protein was decreased (P<0.05). In summary, 27nt-miRNA promotes 
      mesenchymal stem cells differentiation into vascular smooth muscle cells and 
      inhibits cells viability.
FAU - Shen, Feng
AU  - Shen F
AD  - College of Pharmaceutical Sciences, Guangxi Medical University, Nanning 530021, 
      Guangxi, China.
FAU - Yang, Peng
AU  - Yang P
AD  - College of Pharmaceutical Sciences, Guangxi Medical University, Nanning 530021, 
      Guangxi, China.
FAU - Tao, Xiaojing
AU  - Tao X
AD  - College of Pharmaceutical Sciences, Guangxi Medical University, Nanning 530021, 
      Guangxi, China.
FAU - Yan, Yuanyuan
AU  - Yan Y
AD  - College of Pharmaceutical Sciences, Guangxi Medical University, Nanning 530021, 
      Guangxi, China.
FAU - Li, Dan
AU  - Li D
AD  - College of Pharmaceutical Sciences, Guangxi Medical University, Nanning 530021, 
      Guangxi, China.
FAU - Ou, Hesheng
AU  - Ou H
AD  - College of Pharmaceutical Sciences, Guangxi Medical University, Nanning 530021, 
      Guangxi, China.
AD  - The Affiliated Guangxi International Zhuang Hospital, Guangxi Traditional Chinese 
      Medicine, Nanning 530001, Guangxi, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Sheng Wu Gong Cheng Xue Bao
JT  - Sheng wu gong cheng xue bao = Chinese journal of biotechnology
JID - 9426463
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Humans
MH  - *Mesenchymal Stem Cells
MH  - MicroRNAs
MH  - Muscle, Smooth, Vascular
MH  - Myocytes, Smooth Muscle
OTO - NOTNLM
OT  - 27nt-miRNA
OT  - SM22alpha
OT  - SMA
OT  - mesenchymal stem cells
OT  - vascular smooth muscle cells
EDAT- 2019/02/26 06:00
MHDA- 2019/06/14 06:00
CRDT- 2019/02/27 06:00
PHST- 2019/02/27 06:00 [entrez]
PHST- 2019/02/26 06:00 [pubmed]
PHST- 2019/06/14 06:00 [medline]
AID - 10.13345/j.cjb.180243 [doi]
PST - ppublish
SO  - Sheng Wu Gong Cheng Xue Bao. 2019 Feb 25;35(2):290-297. doi: 
      10.13345/j.cjb.180243.