PMID- 30809231
OWN - NLM
STAT- MEDLINE
DCOM- 20201027
LR  - 20210121
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 10
DP  - 2019
TI  - Expression of the Phosphatase Ppef2 Controls Survival and Function of CD8(+) 
      Dendritic Cells.
PG  - 222
LID - 10.3389/fimmu.2019.00222 [doi]
LID - 222
AB  - Apoptotic cell death of Dendritic cells (DCs) is critical for immune homeostasis. 
      Although intrinsic mechanisms controlling DC death have not been fully 
      characterized up to now, experimentally enforced inhibition of DC-death causes 
      various autoimmune diseases in model systems. We have generated mice deficient 
      for Protein Phosphatase with EF-Hands 2 (Ppef2), which is selectively expressed 
      in CD8(+) DCs, but not in other related DC subtypes such as tissue CD103(+) DCs. 
      Ppef2 is down-regulated rapidly upon maturation of DCs by toll-like receptor 
      stimuli, but not upon triggering of CD40. Ppef2-deficient CD8(+) DCs accumulate 
      the pro-apoptotic Bcl-2-like protein 11 (Bim) and show increased apoptosis and 
      reduced competitve repopulation capacities. Furthermore, Ppef2(-/-) CD8(+) DCs 
      have strongly diminished antigen presentation capacities in vivo, as CD8(+) T 
      cells primed by Ppef2(-/-) CD8(+) DCs undergo reduced expansion. In conclusion, 
      our data suggests that Ppef2 is crucial to support survival of immature CD8(+) 
      DCs, while Ppef2 down-regulation during DC-maturation limits T cell responses.
FAU - Zwick, Markus
AU  - Zwick M
AD  - Faculty of Medicine, Biomedical Center (BMC), Institute for Immunology, LMU 
      Munich, Planegg-Martinsried, Germany.
FAU - Ulas, Thomas
AU  - Ulas T
AD  - Life and Medical Sciences Institute, Bonn, Germany.
FAU - Cho, Yi-Li
AU  - Cho YL
AD  - Institute for Medical Microbiology, Immunology and Hygiene, Technische 
      Universitat Munchen, Munich, Germany.
FAU - Ried, Christine
AU  - Ried C
AD  - Faculty of Medicine, Biomedical Center (BMC), Institute for Immunology, LMU 
      Munich, Planegg-Martinsried, Germany.
FAU - Grosse, Leonie
AU  - Grosse L
AD  - Faculty of Medicine, Biomedical Center (BMC), Institute for Immunology, LMU 
      Munich, Planegg-Martinsried, Germany.
FAU - Simon, Charlotte
AU  - Simon C
AD  - Faculty of Medicine, Biomedical Center (BMC), Institute for Immunology, LMU 
      Munich, Planegg-Martinsried, Germany.
FAU - Bernhard, Caroline
AU  - Bernhard C
AD  - Faculty of Medicine, Biomedical Center (BMC), Institute for Immunology, LMU 
      Munich, Planegg-Martinsried, Germany.
FAU - Busch, Dirk H
AU  - Busch DH
AD  - Institute for Medical Microbiology, Immunology and Hygiene, Technische 
      Universitat Munchen, Munich, Germany.
FAU - Schultze, Joachim L
AU  - Schultze JL
AD  - Life and Medical Sciences Institute, Bonn, Germany.
AD  - PRECISE-Platform for Single Cell Genomics and Epigenomics at the German Center 
      for Neurodegenerative Diseases (DZNE) and the University of Bonn, Bonn, Germany.
FAU - Buchholz, Veit R
AU  - Buchholz VR
AD  - Institute for Medical Microbiology, Immunology and Hygiene, Technische 
      Universitat Munchen, Munich, Germany.
FAU - Stutte, Susanne
AU  - Stutte S
AD  - Faculty of Medicine, Biomedical Center (BMC), Institute for Immunology, LMU 
      Munich, Planegg-Martinsried, Germany.
FAU - Brocker, Thomas
AU  - Brocker T
AD  - Faculty of Medicine, Biomedical Center (BMC), Institute for Immunology, LMU 
      Munich, Planegg-Martinsried, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190212
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Bcl-2-Like Protein 11)
RN  - EC 3.1.3.16 (Phosphoprotein Phosphatases)
RN  - EC 3.1.3.16 (Ppef2 protein, mouse)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Apoptosis
MH  - Bcl-2-Like Protein 11/genetics/metabolism
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Cross-Priming
MH  - Dendritic Cells/*immunology
MH  - Homeostasis
MH  - Lymphocyte Activation
MH  - Mice
MH  - Mice, Knockout
MH  - Phosphoprotein Phosphatases/genetics/*metabolism
PMC - PMC6379467
OTO - NOTNLM
OT  - CD8 T cell priming
OT  - DC-maturation
OT  - apoptosis
OT  - cross-presentation
OT  - dendritic cells
OT  - immune homeostasis
EDAT- 2019/02/28 06:00
MHDA- 2020/10/28 06:00
PMCR- 2019/01/01
CRDT- 2019/02/28 06:00
PHST- 2018/09/18 00:00 [received]
PHST- 2019/01/25 00:00 [accepted]
PHST- 2019/02/28 06:00 [entrez]
PHST- 2019/02/28 06:00 [pubmed]
PHST- 2020/10/28 06:00 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2019.00222 [doi]
PST - epublish
SO  - Front Immunol. 2019 Feb 12;10:222. doi: 10.3389/fimmu.2019.00222. eCollection 
      2019.