PMID- 30809692
OWN - NLM
STAT- MEDLINE
DCOM- 20190424
LR  - 20191210
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 411
IP  - 11
DP  - 2019 Apr
TI  - Measurement of serum progesterone by isotope dilution liquid chromatography 
      tandem mass spectrometry: a candidate reference method and its application to 
      evaluating immunoassays.
PG  - 2363-2371
LID - 10.1007/s00216-019-01676-7 [doi]
AB  - Measurement of serum progesterone is important in determining ovarian function. 
      Most progesterone measurements are performed with immunoassays and results are 
      often variable. Standardization of the measurements requires a reliable reference 
      method. An isotope dilution liquid chromatography tandem mass spectrometry 
      (ID-LC/MS/MS) method for the measurement of serum progesterone was developed. 
      Serum samples were spiked with (13)C(3)-progesterone, extracted with a three-step 
      liquid-liquid extraction, and analyzed by LC/MS/MS. A bracketing calibration was 
      used for the analysis and samples were prepared gravimetrically. The developed 
      method showed intra-run and total relative standard deviations (RSDs) of 
      0.50~0.58% and 0.71~1.33%, respectively. The analytical recoveries were 
      99.08~101.50%. Measurement results on certified reference materials obtained with 
      this method agreed with the certified values within the stated measurement 
      uncertainties. The method was applied to evaluate immunoassays through 
      split-sample comparisons. A panel of 48 fresh frozen individual samples were 
      measured with the ID-LC/MS/MS method, and six immunoassays and results were 
      compared. Significant calibration biases and sample-specific deviations were 
      observed on some of the immunoassays.
FAU - Wang, Yufei
AU  - Wang Y
AD  - Graduate School of Peking Union Medical College and Chinese Academy of Medical 
      Sciences, 9 Dongdan Santiao, Beijing, 100730, China.
AD  - National Center for Clinical Laboratories, Beijing Hospital and National Center 
      of Gerontology, 1 Dahua Road, Beijing, 100730, China.
AD  - Beijing Engineering Research Center of Laboratory Medicine, 1 Dahua Road, 
      Beijing, 100730, China.
FAU - Zhang, Tianjiao
AU  - Zhang T
AD  - National Center for Clinical Laboratories, Beijing Hospital and National Center 
      of Gerontology, 1 Dahua Road, Beijing, 100730, China.
AD  - Beijing Engineering Research Center of Laboratory Medicine, 1 Dahua Road, 
      Beijing, 100730, China.
FAU - Zhao, Haijian
AU  - Zhao H
AD  - National Center for Clinical Laboratories, Beijing Hospital and National Center 
      of Gerontology, 1 Dahua Road, Beijing, 100730, China.
AD  - Beijing Engineering Research Center of Laboratory Medicine, 1 Dahua Road, 
      Beijing, 100730, China.
FAU - Zhou, Weiyan
AU  - Zhou W
AD  - National Center for Clinical Laboratories, Beijing Hospital and National Center 
      of Gerontology, 1 Dahua Road, Beijing, 100730, China.
AD  - Beijing Engineering Research Center of Laboratory Medicine, 1 Dahua Road, 
      Beijing, 100730, China.
FAU - Zeng, Jie
AU  - Zeng J
AD  - National Center for Clinical Laboratories, Beijing Hospital and National Center 
      of Gerontology, 1 Dahua Road, Beijing, 100730, China.
AD  - Beijing Engineering Research Center of Laboratory Medicine, 1 Dahua Road, 
      Beijing, 100730, China.
FAU - Zhang, Jiangtao
AU  - Zhang J
AD  - National Center for Clinical Laboratories, Beijing Hospital and National Center 
      of Gerontology, 1 Dahua Road, Beijing, 100730, China.
AD  - Beijing Engineering Research Center of Laboratory Medicine, 1 Dahua Road, 
      Beijing, 100730, China.
FAU - Zhang, Chuanbao
AU  - Zhang C
AD  - Graduate School of Peking Union Medical College and Chinese Academy of Medical 
      Sciences, 9 Dongdan Santiao, Beijing, 100730, China. cbzhang@nccl.org.cn.
AD  - National Center for Clinical Laboratories, Beijing Hospital and National Center 
      of Gerontology, 1 Dahua Road, Beijing, 100730, China. cbzhang@nccl.org.cn.
AD  - Beijing Engineering Research Center of Laboratory Medicine, 1 Dahua Road, 
      Beijing, 100730, China. cbzhang@nccl.org.cn.
FAU - Chen, Wenxiang
AU  - Chen W
AD  - Graduate School of Peking Union Medical College and Chinese Academy of Medical 
      Sciences, 9 Dongdan Santiao, Beijing, 100730, China. wxchen@nccl.org.cn.
AD  - National Center for Clinical Laboratories, Beijing Hospital and National Center 
      of Gerontology, 1 Dahua Road, Beijing, 100730, China. wxchen@nccl.org.cn.
AD  - Beijing Engineering Research Center of Laboratory Medicine, 1 Dahua Road, 
      Beijing, 100730, China. wxchen@nccl.org.cn.
LA  - eng
GR  - No. 2011AA02A102/National High Technology Research and Development Program of 
      China/
GR  - No. Z161100001816043/The Leaping Development Project of Beijing Biomedical 
      Industry/
PT  - Journal Article
PT  - Validation Study
DEP - 20190226
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 4G7DS2Q64Y (Progesterone)
SB  - IM
MH  - Calibration
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Humans
MH  - Immunoassay/methods
MH  - Indicator Dilution Techniques
MH  - Limit of Detection
MH  - Progesterone/*blood
MH  - Reproducibility of Results
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - ID-LC/MS/MS
OT  - Immunoassays
OT  - Progesterone
OT  - Reference method
EDAT- 2019/02/28 06:00
MHDA- 2019/04/25 06:00
CRDT- 2019/02/28 06:00
PHST- 2018/11/12 00:00 [received]
PHST- 2019/02/06 00:00 [accepted]
PHST- 2019/01/26 00:00 [revised]
PHST- 2019/02/28 06:00 [pubmed]
PHST- 2019/04/25 06:00 [medline]
PHST- 2019/02/28 06:00 [entrez]
AID - 10.1007/s00216-019-01676-7 [pii]
AID - 10.1007/s00216-019-01676-7 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2019 Apr;411(11):2363-2371. doi: 10.1007/s00216-019-01676-7. 
      Epub 2019 Feb 26.