PMID- 30812450
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 0191-2917 (Print)
IS  - 0191-2917 (Linking)
VI  - 88
IP  - 1
DP  - 2004 Jan
TI  - Viruses Infecting Cassava in Kenya.
PG  - 17-22
LID - 10.1094/PDIS.2004.88.1.17 [doi]
AB  - A survey of cassava viruses was conducted in major cassava-growing regions of 
      Kenya. A total of 185 leaf samples and 62 stem cuttings from plants with viral 
      disease symptoms were collected and analyzed by biological, electron microscopy, 
      enzyme-linked immunosorbent assay, and polymerase chain reaction. All samples 
      from western Kenya had cassava begomoviruses (African cassava mosaic virus 
      [ACMV], East African cassava mosaic virus [EACMV], and Uganda variant [EACMV-UG]) 
      in either single or in mixed infection. However, all samples from the Coast 
      region were infected with only EACMV, a begomovirus. In addition, 15 samples had 
      mixed infections of EACMV and three other hitherto unidentified filamentous 
      viruses. The viruses observed were 200, 500, 650, and 750 nm long, respectively. 
      In addition to rod-shaped and some flexuous viruses, as seen in a crude sap 
      preparation, pinwheels also were observed, indicating a possible association of 
      some of the viruses with the Potyviridae family. The symptoms induced by these 
      viruses in Nicotiana benthamiana were very severe and often caused about 50% 
      death of the test plants. Back inoculation onto cassava resulted in 100% 
      infections. This finding provides evidence that, other than begomoviruses that 
      cause serious diseases of cassava in Africa, filamentous viruses also are present 
      and, despite their limited distribution, they could reach local significance and, 
      most probably, be as serious as begomoviruses. The implications of these findings 
      are discussed and recommendations for future work suggested.
FAU - Were, H K
AU  - Were HK
AD  - Laboratory of Plant Pathology, Faculty of Agriculture, Graduate School of Kyushu 
      University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
FAU - Winter, S
AU  - Winter S
AD  - DSMZ c/o BBA, Messeweg 11/12, 38104 Braunschweig, Germany.
FAU - Maiss, E
AU  - Maiss E
AD  - Institute of Plant Diseases and Plant Protection, University of Hanover, 
      Herrenhauser Strasse 2, 30419 Hanover, Germany.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Plant Dis
JT  - Plant disease
JID - 9882809
OTO - NOTNLM
OT  - CBSV
OT  - CMD
OT  - SDS-PAGE
OT  - carlavirus
OT  - incidence
EDAT- 2004/01/01 00:00
MHDA- 2004/01/01 00:01
CRDT- 2019/03/01 06:00
PHST- 2019/03/01 06:00 [entrez]
PHST- 2004/01/01 00:00 [pubmed]
PHST- 2004/01/01 00:01 [medline]
AID - 10.1094/PDIS.2004.88.1.17 [doi]
PST - ppublish
SO  - Plant Dis. 2004 Jan;88(1):17-22. doi: 10.1094/PDIS.2004.88.1.17.