PMID- 3081327
OWN - NLM
STAT- MEDLINE
DCOM- 19860402
LR  - 20181130
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 118
IP  - 3
DP  - 1986 Mar
TI  - Identification of a putative GH-releasing factor (GRF) batch as predominantly 
      ovine CRF with a small quantity of human GRF.
PG  - 1246-8
AB  - The existence of discordant results regarding the effects of 
      intracerebroventricular (icv) administration of GRF on GH secretion prompted a 
      reexamination of the central actions of GRF and a detailed chemical 
      characterization of the peptide designated as hpGRF-44-NH2. The icv injection of 
      10 micrograms rat (r) GRF to freely-moving rats caused an acute stimulation of GH 
      release, whereas 10 micrograms of the putative hpGRF peptide icv continued to 
      suppress spontaneous GH secretion. Through a series of biochemical and 
      immunologic studies we demonstrate that peptide hpGRF-44-NH2, code number 
      92-81-5G-41-47, is predominantly ovine (o) CRF and also contains a small amount 
      (3-5%) human (h) GRF-44-NH2. We conclude that the major effect of icv 
      administered GRF, at high doses, is to stimulate GH release and that the central 
      actions previously attributed to the hpGRF peptide are, in fact, due to the oCRF 
      component of this compound.
FAU - Tannenbaum, G S
AU  - Tannenbaum GS
FAU - van der Rest, M
AU  - van der Rest M
FAU - Downs, T R
AU  - Downs TR
FAU - Frohman, L A
AU  - Frohman LA
LA  - eng
GR  - AM-30667/AM/NIADDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Amino Acids)
RN  - 0 (Blood Glucose)
RN  - 0 (Peptide Fragments)
RN  - 9002-72-6 (Growth Hormone)
RN  - 9015-71-8 (Corticotropin-Releasing Hormone)
RN  - 9034-39-3 (Growth Hormone-Releasing Hormone)
SB  - IM
MH  - Amino Acids/analysis
MH  - Animals
MH  - Biological Assay
MH  - Blood Glucose/metabolism
MH  - Chromatography, High Pressure Liquid
MH  - Corticotropin-Releasing Hormone/*analysis
MH  - Growth Hormone/metabolism
MH  - Growth Hormone-Releasing Hormone/*analysis/pharmacology
MH  - Humans
MH  - Peptide Fragments/analysis
MH  - Rats
MH  - Sheep
EDAT- 1986/03/01 00:00
MHDA- 1986/03/01 00:01
CRDT- 1986/03/01 00:00
PHST- 1986/03/01 00:00 [pubmed]
PHST- 1986/03/01 00:01 [medline]
PHST- 1986/03/01 00:00 [entrez]
AID - 10.1210/endo-118-3-1246 [doi]
PST - ppublish
SO  - Endocrinology. 1986 Mar;118(3):1246-8. doi: 10.1210/endo-118-3-1246.