PMID- 3081505
OWN - NLM
STAT- MEDLINE
DCOM- 19860404
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 261
IP  - 7
DP  - 1986 Mar 5
TI  - Effect of alpha-D-mannopyranosylmethyl-p-nitrophenyltriazene on hepatic 
      degradation and processing of the N-linked oligosaccharide chains of alpha 1-acid 
      glycoprotein.
PG  - 3457-63
AB  - The effects of alpha-D-mannopyranosylmethyl-p-nitrophenyltriazene (alpha-ManMNT) 
      on the degradation and biosynthesis of oligosaccharide chains on alpha 1-acid 
      glycoprotein (AGP) were studied. Addition of the triazene to a perfused liver 
      prevented the complete degradation of endocytosed 
      N-acetyl[14C]glucosamine-labeled asialo-AGP and caused the accumulation of 
      Man2GlcNAc1 fragments in the lysosome-enriched fraction of the liver homogenate. 
      This compound also reduced the reincorporation of lysosomally derived [14C]GlcNAc 
      into newly secreted glycoproteins. Cultured hepatocytes treated with the 
      inhibitor synthesized and secreted fully glycosylated AGP. However, the N-linked 
      oligosaccharide chains on AGP secreted by the alpha-ManMNT-treated hepatocytes 
      remained sensitive to digestion with endoglycosidase H, were resistant to 
      neuraminidase, and consisted of Man9-7GlcNAc2 structures as analyzed by high 
      resolution Bio-Gel P-4 chromatography. As measured by their resistance to 
      cleavage by endoglycosidase H, the normal processing of all six carbohydrate 
      chains on AGP to the complex form did not completely resume until nearly 24 h 
      after triazene treatment. Since alpha-ManMNT is likely to irreversibly inactivate 
      alpha-D-mannosidases, the return of normal AGP secretory forms after 24 h 
      probably resulted from synthesis of new processing enzymes.
FAU - Docherty, P A
AU  - Docherty PA
FAU - Kuranda, M J
AU  - Kuranda MJ
FAU - Aronson, N N Jr
AU  - Aronson NN Jr
FAU - BeMiller, J N
AU  - BeMiller JN
FAU - Myers, R W
AU  - Myers RW
FAU - Bohn, J A
AU  - Bohn JA
LA  - eng
GR  - AM 33314/AM/NIADDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Alkaloids)
RN  - 0 (Oligosaccharides)
RN  - 0 (Orosomucoid)
RN  - 0 (Triazenes)
RN  - 101396-17-2 (mannopyranosylmethyl-4-nitrophenyltriazene)
RN  - EC 3.2.1.- (Glycoside Hydrolases)
RN  - EC 3.2.1.- (Hexosaminidases)
RN  - EC 3.2.1.18 (Neuraminidase)
RN  - RSY4RK37KQ (Swainsonine)
SB  - IM
MH  - Alkaloids/pharmacology
MH  - Animals
MH  - Chromatography, Gel
MH  - Glycoside Hydrolases/metabolism
MH  - Hexosaminidases/metabolism
MH  - Liver/drug effects/*metabolism
MH  - Male
MH  - Neuraminidase/metabolism
MH  - Oligosaccharides/*metabolism
MH  - Orosomucoid/*metabolism
MH  - Perfusion
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Swainsonine
MH  - Triazenes/*pharmacology
EDAT- 1986/03/05 00:00
MHDA- 2001/03/28 10:01
CRDT- 1986/03/05 00:00
PHST- 1986/03/05 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1986/03/05 00:00 [entrez]
AID - S0021-9258(17)35805-2 [pii]
PST - ppublish
SO  - J Biol Chem. 1986 Mar 5;261(7):3457-63.