PMID- 30816461
OWN - NLM
STAT- MEDLINE
DCOM- 20190730
LR  - 20200309
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 19
IP  - 4
DP  - 2019 Apr
TI  - Early intervention with gastrodin reduces striatal neurotoxicity in adult rats 
      with experimentally‑induced diabetes mellitus.
PG  - 3114-3122
LID - 10.3892/mmr.2019.9954 [doi]
AB  - Glutamate‑induced excitotoxicity in the striatum has an important role in 
      neurodegenerative diseases. It has been reported that diabetes mellitus (DM) 
      induces excitotoxicity in striatal neurons, although the underlying mechanism 
      remains to be fully elucidated. The present study aimed to investigate the effect 
      of gastrodin on DM‑induced excitotoxicity in the striatal neurons of diabetic 
      rats. Adult Sprague‑Dawley rats were divided into control, diabetic, and 
      gastrodin intervention groups. Diabetes in the rats was induced with a single 
      intraperitoneal injection of streptozotocin (65 mg/kg). In the gastrodin groups, 
      the rats were gavaged with 60 or 120 mg/kg/day gastrodin for 6 weeks, 3 weeks 
      following the induction of diabetes. Pathological alterations in the striatum 
      were assessed using hematoxylin and eosin (H&E) staining. The protein expression 
      levels of phosphorylated (p)‑extracellular signal‑regulated kinase (ERK)1/2, 
      p‑mitogen‑activated protein kinase kinase (MEK)1/2, tyrosine receptor kinase B 
      (TrKB) and brain‑derived neurotrophic factor (BDNF) in the striatal neurons were 
      evaluated by western blotting and double immunofluorescence. Additionally, the 
      extracellular levels of glutamate were measured by microanalysis followed by 
      high‑pressure‑liquid‑chromatography. In diabetic rats, striatal neuronal 
      degeneration was evident following H&E staining, which revealed the common 
      occurrence of pyknotic nuclei. This was coupled with an increase in glutamate 
      levels in the striatal tissues. The protein expression levels of p‑ERK1/2, 
      p‑MEK1/2, TrKB and BDNF in the striatal tissues were significantly increased in 
      the diabetic rats compared with those in the normal rats. In the gastrodin 
      groups, degeneration of the striatal neurons was ameliorated. Furthermore, the 
      expression levels of glutamate, p‑ERK1/2, p‑MEK1/2, TrKB and BDNF in the striatal 
      neurons were decreased. From these findings, it was concluded that reduced 
      neurotoxicity in striatal neurons following treatment with gastrodin may be 
      attributed to its suppressive effects on the expression of p‑ERK1/2, p‑MEK1/2, 
      BDNF and TrKB.
FAU - Qi, Yu-Han
AU  - Qi YH
AD  - Department of Pathology and Pathophysiology, Faculty of Basic Medical Sciences, 
      Kunming Medical University, Kunming, Yunnan 650500, P.R. China.
FAU - Zhu, Rui
AU  - Zhu R
AD  - Department of Pathology and Pathophysiology, Faculty of Basic Medical Sciences, 
      Kunming Medical University, Kunming, Yunnan 650500, P.R. China.
FAU - Wang, Qing
AU  - Wang Q
AD  - Department of Pathology and Pathophysiology, Faculty of Basic Medical Sciences, 
      Kunming Medical University, Kunming, Yunnan 650500, P.R. China.
FAU - Li, Qian
AU  - Li Q
AD  - Department of Pathology and Pathophysiology, Faculty of Basic Medical Sciences, 
      Kunming Medical University, Kunming, Yunnan 650500, P.R. China.
FAU - Liu, Yi-Dan
AU  - Liu YD
AD  - Institute of Drug Discovery and Development, Kunming Pharmaceutical Corporation, 
      Kunming, Yunnan 650500, P.R. China.
FAU - Qian, Zhong-Yi
AU  - Qian ZY
AD  - Department of Morphological Laboratory, Faculty of Basic Medical Sciences, 
      Kunming Medical University, Kunming, Yunnan 650500, P.R. China.
FAU - Yang, Zhi-Hong
AU  - Yang ZH
AD  - Department of Pathology and Pathophysiology, Faculty of Basic Medical Sciences, 
      Kunming Medical University, Kunming, Yunnan 650500, P.R. China.
FAU - Mu, Zhi-Hao
AU  - Mu ZH
AD  - Department of Pathology and Pathophysiology, Faculty of Basic Medical Sciences, 
      Kunming Medical University, Kunming, Yunnan 650500, P.R. China.
FAU - Liu, Xin-Jie
AU  - Liu XJ
AD  - Department of Pathology and Pathophysiology, Faculty of Basic Medical Sciences, 
      Kunming Medical University, Kunming, Yunnan 650500, P.R. China.
FAU - Zhang, Mei-Yan
AU  - Zhang MY
AD  - Department of Pathology and Pathophysiology, Faculty of Basic Medical Sciences, 
      Kunming Medical University, Kunming, Yunnan 650500, P.R. China.
FAU - Wang, Xie
AU  - Wang X
AD  - Department of Pathology and Pathophysiology, Faculty of Basic Medical Sciences, 
      Kunming Medical University, Kunming, Yunnan 650500, P.R. China.
FAU - Liao, Xin-Yu
AU  - Liao XY
AD  - Department of Pathology and Pathophysiology, Faculty of Basic Medical Sciences, 
      Kunming Medical University, Kunming, Yunnan 650500, P.R. China.
FAU - Wan, Qi
AU  - Wan Q
AD  - Institute of Neuroregeneration and Neurorehabilitation, Department of 
      Neurosurgery of The Affiliated Hospital, Qingdao University, Qingdao, Shandong 
      266071, P.R. China.
FAU - Lu, Di
AU  - Lu D
AD  - Biomedical Engineering Research Center, Kunming Medical University, Kunming, 
      Yunnan 650500, P.R. China.
FAU - Zou, Ying-Ying
AU  - Zou YY
AD  - Department of Pathology and Pathophysiology, Faculty of Basic Medical Sciences, 
      Kunming Medical University, Kunming, Yunnan 650500, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190214
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Benzyl Alcohols)
RN  - 0 (Biomarkers)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Glucosides)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 5YS9U2W3RQ (gastrodin)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
MH  - Animals
MH  - Benzyl Alcohols/*metabolism
MH  - Biomarkers
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Corpus Striatum/*metabolism
MH  - Diabetes Mellitus, Experimental/*etiology/*metabolism/pathology
MH  - Gene Expression
MH  - Glucosides/*metabolism
MH  - Glutamic Acid/metabolism
MH  - Male
MH  - Mitogen-Activated Protein Kinase 1/metabolism
MH  - Mitogen-Activated Protein Kinase 3/metabolism
MH  - Models, Biological
MH  - Neurons/metabolism
MH  - Rats
MH  - Receptor, trkB/metabolism
PMC - PMC6423552
EDAT- 2019/03/01 06:00
MHDA- 2019/07/31 06:00
PMCR- 2019/02/14
CRDT- 2019/03/01 06:00
PHST- 2018/06/28 00:00 [received]
PHST- 2019/01/11 00:00 [accepted]
PHST- 2019/03/01 06:00 [pubmed]
PHST- 2019/07/31 06:00 [medline]
PHST- 2019/03/01 06:00 [entrez]
PHST- 2019/02/14 00:00 [pmc-release]
AID - mmr-19-04-3114 [pii]
AID - 10.3892/mmr.2019.9954 [doi]
PST - ppublish
SO  - Mol Med Rep. 2019 Apr;19(4):3114-3122. doi: 10.3892/mmr.2019.9954. Epub 2019 Feb 
      14.