PMID- 30819199
OWN - NLM
STAT- MEDLINE
DCOM- 20200420
LR  - 20231006
IS  - 1479-5876 (Electronic)
IS  - 1479-5876 (Linking)
VI  - 17
IP  - 1
DP  - 2019 Feb 28
TI  - The biological behavior optimization of human periodontal ligament stem cells via 
      preconditioning by the combined application of fibroblast growth factor-2 and 
      A83-01 in in vitro culture expansion.
PG  - 66
LID - 10.1186/s12967-019-1799-1 [doi]
LID - 66
AB  - BACKGROUND: As the optimal source of seed cells in periodontal tissue 
      engineering, periodontal ligament stem cells (PDLSCs) have always been researched 
      to improve cell expansion due to their limited resource and spontaneous 
      differentiation in vitro cultivation. Fibroblast growth factor-2 (FGF-2) has been 
      proven to stimulate bone marrow mesenchymal stem cells (BMMSCs) proliferation and 
      maintain their pluripotency when being added to the culture medium. As a small 
      molecule inhibitor of transforming growth factor-beta receptors (TGF-betaRs), A83-01 
      can also promote cell proliferation. Therefore, the aim of this study was to 
      verify whether the combined application of FGF-2 and A83-01 could augment cell 
      quantity and quality during in vitro culture. METHODS: PDLSCs were preconditioned 
      with A83-01, FGF-2, or their combination. A cell counting kit-8 (CCK8) assay, 
      cell apoptosis assay, ALP activity assay, Alizarin Red S staining assay, RT-PCR 
      assay, Western blot assay and ELISA were used to determine the sustained effects 
      of different preconditioning strategies on the proliferation, apoptosis, 
      stemness, osteogenic differentiation and paracrine action of PDLSCs. RESULTS: The 
      combined application of FGF-2 and A83-01 significantly augmented cell expansion, 
      reduced cell apoptosis, magnified stemness expression, promoted later osteogenic 
      differentiation and mineralization and increased paracrine action of PDLSCs 
      compared with the control. Moreover, the combination presented significant 
      advantages in enhancing proliferation, stemness expression and paracrine action 
      over FGF-2 alone. CONCLUSIONS: The combined application of A83-01 and FGF-2 may 
      be an improved strategy for PDLSCs biological behavior optimization in culture 
      expansion and advantageous for reinforcing proliferation, stemness expression and 
      cytokine secretion over FGF-2 alone.
FAU - Zhang, Chunshu
AU  - Zhang C
AD  - Department of Periodontology, School of Stomatology, Shandong University, 44 West 
      Wenhua Road, Jinan, 250012, Shandong, People's Republic of China.
AD  - Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan, China.
FAU - Guo, Hongmei
AU  - Guo H
AD  - Department of Periodontology, School of Stomatology, Shandong University, 44 West 
      Wenhua Road, Jinan, 250012, Shandong, People's Republic of China.
FAU - Yang, Chengzhe
AU  - Yang C
AD  - Department of Oral and Maxillofacial Surgery, Qilu Hospital and Institute of 
      Stomatology, Shandong University, Jinan, 250012, Shandong, People's Republic of 
      China.
FAU - Chen, Qian
AU  - Chen Q
AD  - Department of Periodontology, School of Stomatology, Shandong University, 44 West 
      Wenhua Road, Jinan, 250012, Shandong, People's Republic of China.
FAU - Huang, Jiahui
AU  - Huang J
AD  - Department of Periodontology, School of Stomatology, Shandong University, 44 West 
      Wenhua Road, Jinan, 250012, Shandong, People's Republic of China.
FAU - Liu, Lianlian
AU  - Liu L
AD  - Department of Periodontology, School of Stomatology, Shandong University, 44 West 
      Wenhua Road, Jinan, 250012, Shandong, People's Republic of China.
FAU - Zhang, Yu
AU  - Zhang Y
AD  - Department of Periodontology, School of Stomatology, Shandong University, 44 West 
      Wenhua Road, Jinan, 250012, Shandong, People's Republic of China.
FAU - Jin, Shanshan
AU  - Jin S
AD  - Department of Periodontology, School of Stomatology, Shandong University, 44 West 
      Wenhua Road, Jinan, 250012, Shandong, People's Republic of China.
FAU - Song, Aimei
AU  - Song A
AD  - Department of Periodontology, School of Stomatology, Shandong University, 44 West 
      Wenhua Road, Jinan, 250012, Shandong, People's Republic of China. 
      sam1972@sdu.edu.cn.
AD  - Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan, China. 
      sam1972@sdu.edu.cn.
FAU - Yang, Pishan
AU  - Yang P
AUID- ORCID: 0000-0002-7326-9452
AD  - Department of Periodontology, School of Stomatology, Shandong University, 44 West 
      Wenhua Road, Jinan, 250012, Shandong, People's Republic of China. 
      yangps@sdu.edu.cn.
AD  - Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan, China. 
      yangps@sdu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190228
PL  - England
TA  - J Transl Med
JT  - Journal of translational medicine
JID - 101190741
RN  - 0 (A-83-01)
RN  - 0 (Cytokines)
RN  - 0 (Pyrazoles)
RN  - 0 (Thiosemicarbazones)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adult
MH  - Alkaline Phosphatase/metabolism
MH  - Apoptosis/drug effects
MH  - Calcification, Physiologic/drug effects
MH  - Calcium/metabolism
MH  - Cell Culture Techniques/*methods
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Fibroblast Growth Factor 2/*pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Osteogenesis/drug effects
MH  - Paracrine Communication/drug effects
MH  - Periodontal Ligament/*cytology
MH  - Pyrazoles/*pharmacology
MH  - Stem Cells/*cytology/drug effects/metabolism
MH  - Thiosemicarbazones/*pharmacology
MH  - Young Adult
PMC - PMC6396448
OTO - NOTNLM
OT  - A83-01
OT  - Cell expansion
OT  - Fibroblast growth factor-2
OT  - Osteogenic differentiation
OT  - Paracrine action
OT  - Periodontal ligament stem cells
EDAT- 2019/03/02 06:00
MHDA- 2020/04/21 06:00
PMCR- 2019/02/28
CRDT- 2019/03/02 06:00
PHST- 2018/11/02 00:00 [received]
PHST- 2019/02/14 00:00 [accepted]
PHST- 2019/03/02 06:00 [entrez]
PHST- 2019/03/02 06:00 [pubmed]
PHST- 2020/04/21 06:00 [medline]
PHST- 2019/02/28 00:00 [pmc-release]
AID - 10.1186/s12967-019-1799-1 [pii]
AID - 1799 [pii]
AID - 10.1186/s12967-019-1799-1 [doi]
PST - epublish
SO  - J Transl Med. 2019 Feb 28;17(1):66. doi: 10.1186/s12967-019-1799-1.