PMID- 30822669
OWN - NLM
STAT- MEDLINE
DCOM- 20190503
LR  - 20190503
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 174
DP  - 2019 Jun 15
TI  - Toxicity of perfluorooctane sulfonate on Phanerochaete chrysosporium: Growth, 
      pollutant degradation and transcriptomics.
PG  - 66-74
LID - S0147-6513(19)30225-8 [pii]
LID - 10.1016/j.ecoenv.2019.02.066 [doi]
AB  - As a persistent organic pollutant listed in the Stockholm Convention, 
      perfluorooctane sulfonate (PFOS) is extremely refractory to degradation under 
      ambient conditions. Its potential ecotoxicity has aroused great concerns and 
      research interests. However, little is known about the toxicity of PFOS on 
      fungus. In this study, the white rot fungus Phanerochaete chrysosporium (P. 
      chrysosporium) was adopted to assess the toxicity of PFOS in liquid culture. The 
      addition of 100 mg/L PFOS potassium salt significantly decreased the fungal 
      biomass by up to 76.4% comparing with un-amended control during the incubation 
      period. The hyphostroma of P. chrysosporium was wizened and its cell membrane was 
      thickened, while its vesicle structure was increased, based on the observation 
      with scanning electron microscope (SEM) and transmission electron microscope 
      (TEM). Nevertheless, the PFOS dosage of below 100 mg/L did not show a 
      considerable damage to the growth of P. chrysosporium. The degradation of 
      malachite green (MG) and 2,4-dichlorophenol (2,4-DCP) by P. chrysosporium was 
      negatively affected by PFOS. At the initial dosage of 100 mg/L PFOS, the 
      decolorization efficiency of MG and the degradation efficiency of 2,4-DCP 
      decreased by 37% and 20%, respectively. This might be attributed to the 
      inhibition of PFOS on MnP and LiP activities. The activities of MnP and LiP 
      decreased by 20.6% and 43.4%, respectively. At a high dosage PFOS (100 mg/L), P. 
      chrysosporium could show a high adsorption of MG but lose its pollutant 
      degradation ability. Transcriptome analysis indicated that PFOS contamination 
      could lead to the change of gene expression in the studied white rot fungus, and 
      the genes regulating membrane structure, cell redox process, and cell transport, 
      synthesis and metabolism were impacted. Membrane damage and oxidative damage were 
      the two main mechanisms of PFOS' toxicity to P. chrysosporium.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Qiao, Weichuan
AU  - Qiao W
AD  - Department of Environmental Engineering, College of Biology and the Environment, 
      Nanjing Forestry University, Nanjing 210037, China.
FAU - Zhang, Yunhao
AU  - Zhang Y
AD  - Department of Environmental Engineering, College of Biology and the Environment, 
      Nanjing Forestry University, Nanjing 210037, China.
FAU - Xie, Zhenyu
AU  - Xie Z
AD  - Department of Environmental Engineering, College of Biology and the Environment, 
      Nanjing Forestry University, Nanjing 210037, China.
FAU - Luo, Yang
AU  - Luo Y
AD  - Department of Environmental Engineering, College of Biology and the Environment, 
      Nanjing Forestry University, Nanjing 210037, China.
FAU - Zhang, Xuansong
AU  - Zhang X
AD  - Department of Environmental Engineering, College of Biology and the Environment, 
      Nanjing Forestry University, Nanjing 210037, China.
FAU - Sang, Cunxing
AU  - Sang C
AD  - Department of Environmental Engineering, College of Biology and the Environment, 
      Nanjing Forestry University, Nanjing 210037, China.
FAU - Xie, Shuguang
AU  - Xie S
AD  - State Key Joint Laboratory of Environmental Simulation and Pollution Control 
      (SKJLESPC), College of Environmental Sciences and Engineering, Peking University, 
      Beijing 100871, China. Electronic address: xiesg@pku.edu.cn.
FAU - Huang, Jun
AU  - Huang J
AD  - State Key Joint Laboratory of Environment Simulation and Pollution Control 
      (SKJLESPC), Beijing Key Laboratory for Emerging Organic Contaminants 
      Control(BKLEOC), School of Environment, POPs Research Center, Tsinghua 
      University, Beijing 100084, China. Electronic address: 
      huangjun@mail.tsinghua.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190226
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (Alkanesulfonic Acids)
RN  - 0 (Chlorophenols)
RN  - 0 (Coloring Agents)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Fluorocarbons)
RN  - 0 (Rosaniline Dyes)
RN  - 12058M7ORO (malachite green)
RN  - 9H2MAI21CL (perfluorooctane sulfonic acid)
RN  - R669TG1950 (2,4-dichlorophenol)
SB  - IM
MH  - Adsorption
MH  - Alkanesulfonic Acids/*toxicity
MH  - Biomass
MH  - Chlorophenols/metabolism
MH  - Coloring Agents/metabolism
MH  - Environmental Pollutants/*metabolism/*toxicity
MH  - Fluorocarbons/*toxicity
MH  - Phanerochaete/*drug effects/genetics/growth & development/metabolism
MH  - Rosaniline Dyes/metabolism
MH  - Transcriptome/*drug effects
OTO - NOTNLM
OT  - 2,4-dichlorophenol
OT  - Decolorization
OT  - Gene expression
OT  - Perfluoroalkyl substances
OT  - Toxic effect
OT  - White rot fungus
EDAT- 2019/03/02 06:00
MHDA- 2019/05/06 06:00
CRDT- 2019/03/02 06:00
PHST- 2018/11/30 00:00 [received]
PHST- 2019/02/18 00:00 [revised]
PHST- 2019/02/20 00:00 [accepted]
PHST- 2019/03/02 06:00 [pubmed]
PHST- 2019/05/06 06:00 [medline]
PHST- 2019/03/02 06:00 [entrez]
AID - S0147-6513(19)30225-8 [pii]
AID - 10.1016/j.ecoenv.2019.02.066 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2019 Jun 15;174:66-74. doi: 10.1016/j.ecoenv.2019.02.066. 
      Epub 2019 Feb 26.