PMID- 30822703
OWN - NLM
STAT- MEDLINE
DCOM- 20191212
LR  - 20191217
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 100
DP  - 2019 Apr
TI  - Effects of two fast-setting pulp-capping materials on cell viability and 
      osteogenic differentiation in human dental pulp stem cells: An in vitro study.
PG  - 100-105
LID - S0003-9969(18)30698-8 [pii]
LID - 10.1016/j.archoralbio.2019.02.014 [doi]
AB  - OBJECTIVE: The purpose of this study was to compare the effects of two 
      fast-setting pulp-capping materials, Biodentine (BD) and iRoot Fast Set (FS) root 
      repair material, on the attachment, viability, migration, and differentiation of 
      human dental pulp stem cells (hDPSCs). METHODS: A comparative study was conducted 
      between BD and FS material disks. Scanning electron microscope (SEM) images were 
      used to observe the attachment of hDPSCs on the disks. A live/dead assay was used 
      to assess the cell viability. Transwell assay was performed to study cell 
      migration. Cell differentiation was determined by quantitative real-time 
      polymerase chain reaction (qRT-PCR) for the analysis of osteogenic 
      differentiation gene expression: alkaline phosphatase (ALP), collagen type I 
      (COL1) and osteocalcin (OCN). RESULTS: SEM images indicated that hDPSCs showed a 
      well-spreading morphology on both BD and FS disks. FS significantly increased the 
      proliferation and migration of hDPSCs on day 7 (P＜0.05). Neither BD nor FS 
      promoted the expression of osteogenic genes during the observation period. 
      CONCLUSIONS: BD and FS both were beneficial to hDPSC attachment, and they had 
      similar effects on cell osteogenic differentiation, whereas FS performed better 
      than BD on hDPSCs proliferation and migration.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Sun, Yan
AU  - Sun Y
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, Department of Conservative Dentistry and Endodontics, West China School 
      and Hospital of Stomatology, Sichuan University, Chengdu, 610041, China; 
      Department of Conservative Dentistry and Endodontics, School & Hospital of 
      Stomatology, Wenzhou Medical University, Wenzhou, 325027, China. Electronic 
      address: 934001560@qq.com.
FAU - Liu, Jun
AU  - Liu J
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, Department of Orthodontics, West China School and Hospital of 
      Stomatology, Sichuan University, Chengdu, 610041, China. Electronic address: 
      junliu@scu.edu.cn.
FAU - Luo, Tao
AU  - Luo T
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, Department of Conservative Dentistry and Endodontics, West China School 
      and Hospital of Stomatology, Sichuan University, Chengdu, 610041, China. 
      Electronic address: 564599687@qq.com.
FAU - Shen, Ya
AU  - Shen Y
AD  - Division of Endodontics, Department of Oral Biological and Medical Sciences, 
      Faculty of Dentistry, University of British Columbia, Vancouver, British 
      Columbia, Canada. Electronic address: yashen@dentistry.ubc.ca.
FAU - Zou, Ling
AU  - Zou L
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, Department of Conservative Dentistry and Endodontics, West China School 
      and Hospital of Stomatology, Sichuan University, Chengdu, 610041, China. 
      Electronic address: zouling@scu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190220
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Calcium Compounds)
RN  - 0 (Pulp Capping and Pulpectomy Agents)
RN  - 0 (Silicates)
RN  - 404G39282C (tricalcium silicate)
SB  - IM
MH  - Calcium Compounds/*pharmacology
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Dental Pulp/*cytology
MH  - Humans
MH  - *Osteogenesis
MH  - Pulp Capping and Pulpectomy Agents/*pharmacology
MH  - Silicates/*pharmacology
MH  - Stem Cells/*cytology/*drug effects
OTO - NOTNLM
OT  - Biodentine (BD)
OT  - Cell viability
OT  - Human dental pulp stem cells (hDPSCs)
OT  - Osteogenic differentiation
OT  - Pulp capping
OT  - iRoot fast set root repair material (FS)
EDAT- 2019/03/02 06:00
MHDA- 2019/12/18 06:00
CRDT- 2019/03/02 06:00
PHST- 2018/10/16 00:00 [received]
PHST- 2019/01/31 00:00 [revised]
PHST- 2019/02/19 00:00 [accepted]
PHST- 2019/03/02 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/03/02 06:00 [entrez]
AID - S0003-9969(18)30698-8 [pii]
AID - 10.1016/j.archoralbio.2019.02.014 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2019 Apr;100:100-105. doi: 10.1016/j.archoralbio.2019.02.014. 
      Epub 2019 Feb 20.