PMID- 30824726
OWN - NLM
STAT- MEDLINE
DCOM- 20200928
LR  - 20200928
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Mar 1
TI  - Variation in genome size, cell and nucleus volume, chromosome number and rDNA 
      loci among duckweeds.
PG  - 3234
LID - 10.1038/s41598-019-39332-w [doi]
LID - 3234
AB  - Duckweeds are small, free-floating, largely asexual and highly neotenous 
      organisms. They display the most rapid growth among flowering plants and are of 
      growing interest in aquaculture and genome biology. Genomic and chromosomal data 
      are still rare. Applying flow-cytometric genome size measurement, microscopic 
      determination of frond, cell and nucleus morphology, as well as fluorescence in 
      situ hybridization (FISH) for localization of ribosomal DNA (rDNA), we compared 
      eleven species, representative for the five duckweed genera to search for 
      potential correlations between genome size, cell and nuclei volume, simplified 
      body architecture (neoteny), chromosome numbers and rDNA loci. We found a 
      ~14-fold genome size variation (from 160 to 2203 Mbp), considerable differences 
      in frond size and shape, highly variable guard cell and nucleus size, chromosome 
      number (from 2n = 36 to 82) and number of 5S and 45S rDNA loci. In general, 
      genome size is positively correlated with guard cell and nucleus volume 
      (p < 0.001) and with the neoteny level and inversely with the frond size. In 
      individual cases these correlations could be blurred for instance by particular 
      body and cell structures which seem to be linked to specific floating styles. 
      Chromosome number and rDNA loci variation between the tested species was 
      independent of the genome size. We could not confirm previously reported 
      intraspecific variation of chromosome numbers between individual clones of the 
      genera Spirodela and Landoltia.
FAU - Hoang, Phuong T N
AU  - Hoang PTN
AD  - Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, 
      D-06466, Seeland, Germany.
AD  - Dalat University, Lamdong Province, Vietnam.
FAU - Schubert, Veit
AU  - Schubert V
AUID- ORCID: 0000-0002-3072-0485
AD  - Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, 
      D-06466, Seeland, Germany.
FAU - Meister, Armin
AU  - Meister A
AD  - Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, 
      D-06466, Seeland, Germany.
FAU - Fuchs, Jorg
AU  - Fuchs J
AD  - Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, 
      D-06466, Seeland, Germany.
FAU - Schubert, Ingo
AU  - Schubert I
AUID- ORCID: 0000-0002-6300-2068
AD  - Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, 
      D-06466, Seeland, Germany. schubert@ipk-gatersleben.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190301
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (DNA, Plant)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 5S)
RN  - 0 (RNA, ribosomal, 45S)
SB  - IM
MH  - Araceae/classification/*genetics
MH  - *Cell Nucleus Size
MH  - Chromosomes, Plant/*genetics
MH  - DNA, Plant/*genetics
MH  - DNA, Ribosomal/*genetics
MH  - Genetic Variation
MH  - *Genome Size
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Fluorescence
MH  - Phylogeny
MH  - Plant Stomata/genetics/metabolism
MH  - RNA, Ribosomal/genetics
MH  - RNA, Ribosomal, 5S/genetics
MH  - Species Specificity
PMC - PMC6397220
COIS- The authors declare no competing interests.
EDAT- 2019/03/03 06:00
MHDA- 2020/09/29 06:00
PMCR- 2019/03/01
CRDT- 2019/03/03 06:00
PHST- 2018/10/11 00:00 [received]
PHST- 2019/01/18 00:00 [accepted]
PHST- 2019/03/03 06:00 [entrez]
PHST- 2019/03/03 06:00 [pubmed]
PHST- 2020/09/29 06:00 [medline]
PHST- 2019/03/01 00:00 [pmc-release]
AID - 10.1038/s41598-019-39332-w [pii]
AID - 39332 [pii]
AID - 10.1038/s41598-019-39332-w [doi]
PST - epublish
SO  - Sci Rep. 2019 Mar 1;9(1):3234. doi: 10.1038/s41598-019-39332-w.