PMID- 30825888
OWN - NLM
STAT- MEDLINE
DCOM- 20190321
LR  - 20190321
IS  - 1532-2971 (Electronic)
IS  - 1090-0233 (Linking)
VI  - 244
DP  - 2019 Feb
TI  - Influence of age, group size and the presence of porcine reproductive and 
      respiratory syndrome virus on the collection of oral fluids.
PG  - 13-15
LID - S1090-0233(18)30769-X [pii]
LID - 10.1016/j.tvjl.2018.12.005 [doi]
AB  - Pen-based oral fluid (OF) samples have been approved for detecting porcine 
      reproductive and respiratory syndrome virus (PRRSV) RNA and antibodies. The aim 
      of the study was to investigate the influence of age, group size and the presence 
      of PRRSV infection on sampling using pen-based OF collected by presenting pigs 
      with a rope to chew. Samples were collected from pigs of 4/5 weeks of age, 7/8 
      weeks of age and 12/14 weeks of age. Subsequent PRRSV-PCR and ELISA analyses of 
      OF and serum samples were performed. In this study, 76.5-81.9% of pigs chewed the 
      rope. The group size had significant impact on the pig-rope-interaction 
      (P=0.047). OF and serum had an almost perfect and significant agreement for the 
      detection of antibodies (kappa=0.829, P<0.001) and a fair and not significant 
      agreement (kappa=0.347, P=0.75) for the detection PRRSV-genome.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Graage, Robert
AU  - Graage R
AD  - Department for Farm Animals and Veterinary Public Health, University Clinic for 
      Swine, University of Veterinary Medicine Vienna, Vienna Vetertinaerplatz 1, 
      A-1220 Vienna, Austria. Electronic address: rgraage@vetclinics.uzh.ch.
FAU - Hennig-Pauka, Isabel
AU  - Hennig-Pauka I
AD  - Department for Farm Animals and Veterinary Public Health, University Clinic for 
      Swine, University of Veterinary Medicine Vienna, Vienna Vetertinaerplatz 1, 
      A-1220 Vienna, Austria.
FAU - Arbinger, Heidelore
AU  - Arbinger H
AD  - Department for Farm Animals and Veterinary Public Health, University Clinic for 
      Swine, University of Veterinary Medicine Vienna, Vienna Vetertinaerplatz 1, 
      A-1220 Vienna, Austria.
FAU - Ritzmann, Mathias
AU  - Ritzmann M
AD  - Clinic for Swine at the Centre for Clinical Veterinary Medicine, 
      Ludwig-Maximilians-University Munich, Sonnenstrasse 16, D-85764 Oberschleissheim, 
      Germany.
FAU - Ladinig, Andrea
AU  - Ladinig A
AD  - Department for Farm Animals and Veterinary Public Health, University Clinic for 
      Swine, University of Veterinary Medicine Vienna, Vienna Vetertinaerplatz 1, 
      A-1220 Vienna, Austria.
LA  - eng
PT  - Journal Article
DEP - 20181207
PL  - England
TA  - Vet J
JT  - Veterinary journal (London, England : 1997)
JID - 9706281
SB  - IM
MH  - Age Factors
MH  - *Animal Husbandry
MH  - Animals
MH  - Austria/epidemiology
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Female
MH  - Male
MH  - Polymerase Chain Reaction/veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/*epidemiology/virology
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & purification
MH  - Saliva/virology
MH  - Specimen Handling/veterinary
MH  - Swine
OTO - NOTNLM
OT  - ELISA
OT  - Oral fluid
OT  - PCR
OT  - PRRSV
OT  - Rope
OT  - Surveillance
EDAT- 2019/03/04 06:00
MHDA- 2019/03/22 06:00
CRDT- 2019/03/04 06:00
PHST- 2018/02/02 00:00 [received]
PHST- 2018/12/05 00:00 [revised]
PHST- 2018/12/06 00:00 [accepted]
PHST- 2019/03/04 06:00 [entrez]
PHST- 2019/03/04 06:00 [pubmed]
PHST- 2019/03/22 06:00 [medline]
AID - S1090-0233(18)30769-X [pii]
AID - 10.1016/j.tvjl.2018.12.005 [doi]
PST - ppublish
SO  - Vet J. 2019 Feb;244:13-15. doi: 10.1016/j.tvjl.2018.12.005. Epub 2018 Dec 7.