PMID- 30826378
OWN - NLM
STAT- MEDLINE
DCOM- 20191121
LR  - 20191121
IS  - 1879-1220 (Electronic)
IS  - 0960-0760 (Linking)
VI  - 189
DP  - 2019 May
TI  - Imaging of vitamin D in psoriatic skin using time-of-flight secondary ion mass 
      spectrometry (ToF-SIMS): A pilot case study.
PG  - 154-160
LID - S0960-0760(18)30642-3 [pii]
LID - 10.1016/j.jsbmb.2019.02.015 [doi]
AB  - Recently it has been recognized that there is a need of investigating in detail 
      the vitamin D synthesis and metabolism directly in the skin with respect to its 
      possible autocrine and paracrine actions. The potential effects the active 
      metabolite of vitamin D exerts in pathological skin conditions like psoriasis 
      needs to be clarified. Under ultraviolet B (UVB) irradiation skin can 
      autonomously synthesize, activate and degrade vitamin D. In this pilot case 
      study, we used for the first time Time-of-flight secondary ion mass spectrometry 
      (ToF-SIMS) in the analysis of skin biopsies from a patient with psoriasis before 
      and after UVB phototherapy. We were able to visualize vitamin D(3) and its 
      metabolites in the skin and subcutaneous tissue. At the same time information 
      about their localization at subcellular level and morphology of the skin was 
      received. This study proves that ToF-SIMS is a promising powerful tool to be used 
      when investigating vitamin D s role in dermatological diseases through skin 
      biopsies.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Siekkeri Vandikas, Maria
AU  - Siekkeri Vandikas M
AD  - Department of Dermatology and Venereology, Institute of Clinical Sciences, 
      Sahlgrenska Academy, University of Gothenburg, SE-413 45 Gothenburg, Sweden. 
      Electronic address: maria.s.vandikas@gu.se.
FAU - Hellstrom, Evelina
AU  - Hellstrom E
AD  - Department of Dermatology and Venereology, Institute of Clinical Sciences, 
      Sahlgrenska Academy, University of Gothenburg, SE-413 45 Gothenburg, Sweden.
FAU - Malmberg, Per
AU  - Malmberg P
AD  - Department of Chemistry and Chemical Engineering, Chalmers University of 
      Technology, SE-412 96 Gothenburg, Sweden.
FAU - Osmancevic, Amra
AU  - Osmancevic A
AD  - Department of Dermatology and Venereology, Institute of Clinical Sciences, 
      Sahlgrenska Academy, University of Gothenburg, SE-413 45 Gothenburg, Sweden; 
      Region Vastra Gotaland, Sahlgrenska University Hospital, Department of 
      Dermatology and Venereology, Gothenburg, Sweden.
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190228
PL  - England
TA  - J Steroid Biochem Mol Biol
JT  - The Journal of steroid biochemistry and molecular biology
JID - 9015483
RN  - 1C6V77QF41 (Cholecalciferol)
SB  - IM
MH  - Aged
MH  - Cholecalciferol/*analysis
MH  - Female
MH  - Humans
MH  - Pilot Projects
MH  - Psoriasis/*pathology/therapy
MH  - Skin/*pathology
MH  - Spectrometry, Mass, Secondary Ion
MH  - Ultraviolet Therapy
OTO - NOTNLM
OT  - Narrow-band UVB
OT  - Psoriasis
OT  - ToF-SIMS
OT  - Vitamin D
EDAT- 2019/03/04 06:00
MHDA- 2019/11/22 06:00
CRDT- 2019/03/04 06:00
PHST- 2018/10/16 00:00 [received]
PHST- 2019/01/22 00:00 [revised]
PHST- 2019/02/27 00:00 [accepted]
PHST- 2019/03/04 06:00 [pubmed]
PHST- 2019/11/22 06:00 [medline]
PHST- 2019/03/04 06:00 [entrez]
AID - S0960-0760(18)30642-3 [pii]
AID - 10.1016/j.jsbmb.2019.02.015 [doi]
PST - ppublish
SO  - J Steroid Biochem Mol Biol. 2019 May;189:154-160. doi: 
      10.1016/j.jsbmb.2019.02.015. Epub 2019 Feb 28.