PMID- 30826524
OWN - NLM
STAT- MEDLINE
DCOM- 20191224
LR  - 20191224
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 404
DP  - 2019 Apr 15
TI  - Acupoint Sensitization is Associated with Increased Excitability and 
      Hyperpolarization-Activated Current (I(h)) in C- But Not Adelta-Type Neurons.
PG  - 499-509
LID - S0306-4522(19)30139-3 [pii]
LID - 10.1016/j.neuroscience.2019.02.028 [doi]
AB  - Under pathological conditions, acupoint sensitization is the phenomenon of 
      acupoints transforming from the stable state to the dynamic state. Evidences 
      suggest that hyperpolarization-activated current (I(h)), conducted by the 
      hyperpolarization-activated/cyclic nucleotide-gated (HCN) channel, greatly 
      contributes to the peripheral and central sensitization. However, the role of the 
      I(h) current in acupoint sensitization has not been explained. In the present 
      study, changes in excitability, I(h) density and the HCN channel of dorsal root 
      ganglion (DRG) nociceptive neurons were examined in the later phase of knee 
      osteoarthritis (KOA) rats. To investigate the neuronal specificity of acupoint 
      sensitization, retrograde dyes were injected into the acupoints ST35 and GB37. 
      The results showed that acupoint sensitization occurred in bilateral ST35 but not 
      GB37 acupoints. The excitability and I(h) density of C- but not A(delta)-type neurons 
      innervating ST35 acupoint increased in bilateral L5 DRG of acupoint sensitized 
      rats than that of sham rats. No obvious changes were found in the excitability or 
      I(h) density of C- and A(delta)-type neurons innervating the GB37 acupoint in the 
      bilateral L5 DRG. HCN channel subtype 2 (HCN2) expression levels significantly 
      increased after acupoint sensitization. Furthermore, ZD7288, an HCN current 
      (I(h)) blocker, attenuated the acupoint sensitization of the ST35 acupoint. Taken 
      together, our findings suggest that the increased excitability of C- but not 
      A(delta)-type neurons and the upregulation of I(h)/HCN2 channels contribute to the 
      formation of acupoint sensitization.
CI  - Copyright (c) 2019 The Author(s). Published by Elsevier Ltd.. All rights reserved.
FAU - Zhang, Ming
AU  - Zhang M
AD  - Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, the 
      Fourth Military Medical University, the 127(th) West Changle Road, Xi'an, 710032, 
      Shaanxi, China; Department of Central Laboratory, the General Hospital of Western 
      Theater Command, the 270(th)Tianhui Road, Chengdu, 610083, Sichuan, China.
FAU - Guo, Haiyun
AU  - Guo H
AD  - Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, the 
      Fourth Military Medical University, the 127(th) West Changle Road, Xi'an, 710032, 
      Shaanxi, China.
FAU - Ma, Yongyuan
AU  - Ma Y
AD  - Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, the 
      Fourth Military Medical University, the 127(th) West Changle Road, Xi'an, 710032, 
      Shaanxi, China.
FAU - Xu, Feifei
AU  - Xu F
AD  - Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, the 
      Fourth Military Medical University, the 127(th) West Changle Road, Xi'an, 710032, 
      Shaanxi, China.
FAU - Bai, Fuhai
AU  - Bai F
AD  - Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, the 
      Fourth Military Medical University, the 127(th) West Changle Road, Xi'an, 710032, 
      Shaanxi, China.
FAU - Liang, Shirui
AU  - Liang S
AD  - Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, the 
      Fourth Military Medical University, the 127(th) West Changle Road, Xi'an, 710032, 
      Shaanxi, China.
FAU - Hu, Huimin
AU  - Hu H
AD  - Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, the 
      Fourth Military Medical University, the 127(th) West Changle Road, Xi'an, 710032, 
      Shaanxi, China.
FAU - Wang, Qi
AU  - Wang Q
AD  - Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, the 
      Fourth Military Medical University, the 127(th) West Changle Road, Xi'an, 710032, 
      Shaanxi, China.
FAU - Deng, Jiao
AU  - Deng J
AD  - Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, the 
      Fourth Military Medical University, the 127(th) West Changle Road, Xi'an, 710032, 
      Shaanxi, China.
FAU - Dong, Hailong
AU  - Dong H
AD  - Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, the 
      Fourth Military Medical University, the 127(th) West Changle Road, Xi'an, 710032, 
      Shaanxi, China.
FAU - Xiong, Lize
AU  - Xiong L
AD  - Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, the 
      Fourth Military Medical University, the 127(th) West Changle Road, Xi'an, 710032, 
      Shaanxi, China. Electronic address: mzkxlz@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190301
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels)
SB  - IM
MH  - *Acupuncture Points
MH  - Animals
MH  - Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels/*physiology
MH  - Male
MH  - Nerve Fibers, Myelinated/*physiology
MH  - Nerve Fibers, Unmyelinated/*physiology
MH  - Neurons/*physiology
MH  - Osteoarthritis, Knee/physiopathology/*therapy
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - C-type neuron
OT  - I(h)
OT  - acupoint sensitization
OT  - dorsal root ganglion
OT  - hyperpolarization-activated/cyclic nucleotide-gated channel
EDAT- 2019/03/04 06:00
MHDA- 2019/12/25 06:00
CRDT- 2019/03/04 06:00
PHST- 2018/04/06 00:00 [received]
PHST- 2019/02/19 00:00 [revised]
PHST- 2019/02/20 00:00 [accepted]
PHST- 2019/03/04 06:00 [pubmed]
PHST- 2019/12/25 06:00 [medline]
PHST- 2019/03/04 06:00 [entrez]
AID - S0306-4522(19)30139-3 [pii]
AID - 10.1016/j.neuroscience.2019.02.028 [doi]
PST - ppublish
SO  - Neuroscience. 2019 Apr 15;404:499-509. doi: 10.1016/j.neuroscience.2019.02.028. 
      Epub 2019 Mar 1.