PMID- 30826543
OWN - NLM
STAT- MEDLINE
DCOM- 20190507
LR  - 20190507
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 174
DP  - 2019 Jun 15
TI  - One-step sample processing method for the determination of perchlorate in human 
      urine, whole blood and breast milk using liquid chromatography tandem mass 
      spectrometry.
PG  - 175-180
LID - S0147-6513(19)30239-8 [pii]
LID - 10.1016/j.ecoenv.2019.02.081 [doi]
AB  - A one-step sample processing was developed to determine the levels of perchlorate 
      in human urine, whole blood and breast milk by using liquid chromatography tandem 
      mass spectrometry (LC-MS/MS). Athena C18-WP column was used to separate and 
      analyze perchlorate. Perchlorate and isotope-labeled perchlorate (Cl(18)O(4)(-)) 
      internal standards were spiked in the sample matrix through vortex mixing, 
      centrifugation, and filtration. The filtrate was collected and subjected to LC 
      analysis. The developed method was validated for its reproducibility, linearity, 
      trueness, and recovery. Satisfactory recovery of perchlorate ranged from 81% to 
      117% with intraday relative standard deviations (RSDs) (n = 3) and inter-day RSDs 
      (n = 9) of 5-18% and of 5-16%, respectively. Good linearity (R(2) >/= 0.99) was 
      observed. Limits of detection and quantification for perchlorate ranged from 
      0.06 microg/L to 0.3 microg/L and from 0.2 microg/L to 1 microg/L, respectively. Perchlorate 
      concentrations were found in human urine (n = 38) and whole blood (n = 8) samples 
      with the range of 6.5-288.6 microg/L and 0.3-2.8 microg/L, respectively. These results 
      indicate the applicability of our developed method in determining perchlorate 
      level in real samples. Moreover, this method is also highly reliable, sensitive 
      and selective in detecting perchlorate in human urine, whole blood and breast 
      milk samples and may be applicable to other matrixes i.e. saliva, serum, plasma, 
      milk powder and dairy milk.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Song, Shiming
AU  - Song S
AD  - School of Environmental Science and Engineering, Sun Yat-Sen University, 
      Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Environmental 
      Pollution Control and Remediation Technology (Sun Yat-Sen University), Guangzhou 
      510275, China.
FAU - Ruan, Jujun
AU  - Ruan J
AD  - School of Environmental Science and Engineering, Sun Yat-Sen University, 
      Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Environmental 
      Pollution Control and Remediation Technology (Sun Yat-Sen University), Guangzhou 
      510275, China.
FAU - Bai, Xueyuan
AU  - Bai X
AD  - School of Environmental Science and Engineering, Sun Yat-Sen University, 
      Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Environmental 
      Pollution Control and Remediation Technology (Sun Yat-Sen University), Guangzhou 
      510275, China.
FAU - Xie, Lei
AU  - Xie L
AD  - School of Environmental Science and Engineering, Sun Yat-Sen University, 
      Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Environmental 
      Pollution Control and Remediation Technology (Sun Yat-Sen University), Guangzhou 
      510275, China.
FAU - Zhang, Bo
AU  - Zhang B
AD  - School of Environmental Science and Engineering, Sun Yat-Sen University, 
      Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Environmental 
      Pollution Control and Remediation Technology (Sun Yat-Sen University), Guangzhou 
      510275, China.
FAU - He, Yuan
AU  - He Y
AD  - School of Environmental Science and Engineering, Sun Yat-Sen University, 
      Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Environmental 
      Pollution Control and Remediation Technology (Sun Yat-Sen University), Guangzhou 
      510275, China.
FAU - Zhang, Tao
AU  - Zhang T
AD  - School of Environmental Science and Engineering, Sun Yat-Sen University, 
      Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Environmental 
      Pollution Control and Remediation Technology (Sun Yat-Sen University), Guangzhou 
      510275, China. Electronic address: zhangt47@mail.sysu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190228
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (Perchlorates)
RN  - VLA4NZX2P4 (perchlorate)
SB  - IM
MH  - Animals
MH  - Chromatography, Liquid/methods
MH  - Environmental Exposure/analysis
MH  - Female
MH  - Humans
MH  - Milk
MH  - Milk, Human/chemistry
MH  - Perchlorates/*analysis/blood/urine
MH  - Reproducibility of Results
MH  - Tandem Mass Spectrometry/methods
MH  - Urinalysis/methods
OTO - NOTNLM
OT  - Breast milk
OT  - Human urine
OT  - LC-MS/MS
OT  - One-step
OT  - Perchlorate
OT  - Whole blood
EDAT- 2019/03/04 06:00
MHDA- 2019/05/08 06:00
CRDT- 2019/03/04 06:00
PHST- 2018/12/14 00:00 [received]
PHST- 2019/02/10 00:00 [revised]
PHST- 2019/02/25 00:00 [accepted]
PHST- 2019/03/04 06:00 [pubmed]
PHST- 2019/05/08 06:00 [medline]
PHST- 2019/03/04 06:00 [entrez]
AID - S0147-6513(19)30239-8 [pii]
AID - 10.1016/j.ecoenv.2019.02.081 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2019 Jun 15;174:175-180. doi: 
      10.1016/j.ecoenv.2019.02.081. Epub 2019 Feb 28.