PMID- 30826652
OWN - NLM
STAT- MEDLINE
DCOM- 20190710
LR  - 20190710
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 131
DP  - 2019 Apr 15
TI  - A sensitive sandwich-type immunosensor for the detection of MCP-1 based on a 
      rGO-TEPA-Thi-Au nanocomposite and novel RuPdPt trimetallic nanoalloy particles.
PG  - 67-73
LID - S0956-5663(19)30124-1 [pii]
LID - 10.1016/j.bios.2019.02.021 [doi]
AB  - A novel electrochemical immunosensor was proposed for the detection of monocyte 
      chemoattractant protein-1 (MCP-1), a biomarker of cardiovascular disease. Due to 
      thionine (Thi) possessing electroactive redox properties, a one-step approach was 
      utilized to synthesize a reduced graphene oxide-tetraethylene-thionine-Au 
      (rGO-TEPA-Thi-Au) nanocomposite at room temperature using the synergistic effect 
      of Thi and rGO-TEPA towards HAuCl(4). We obtained the excellent matrix material, 
      which immobilized more primary antibody MCP-1-Ab(1) on rGO-TEPA on a modified 
      glassy carbon electrode (GCE). To further enhance the sensitivity of the sensor, 
      a novel signal generation and amplification strategy was developed for detection. 
      RuPdPt trimetallic nanoalloy particles (RuPdPt TNPs), a novel nanomaterial, were 
      synthesized by a one-pot method, displayed a uniform morphology as well as good 
      electrochemical activity and bound with the secondary antibodies against MCP-1 
      via the Pt-NH(2) bond. Most importantly, RuPdPt TNPs have a significant ability 
      to catalyze H(2)O(2) to produce an electron. The electrochemical signal was 
      highly amplified because the electrochemical signal was primarily derived from 
      the synergistic catalysis of H(2)O(2) by RuPdPt TNPs and recorded by 
      chronoamperometry. Under the optimal conditions, this newly designed biosensor 
      exhibited sensitive detection of MCP-1 in the range from 20 fg mL(-1) to 
      1000 pg mL(-1), with a detection limit of 8.9 fg mL(-1) (based on a S/N = 3). 
      Additionally, the designed immunosensor showed acceptable selectivity, 
      reproducibility and stability. This immunosensor is a promising strategy for 
      analyzing clinical serum samples in the future.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Mao, Weiran
AU  - Mao W
AD  - College of Pharmacy, Institute of Life Science and School of Public Health, 
      Chongqing Medical University, Chongqing 400016, PR China.
FAU - He, Junlin
AU  - He J
AD  - College of Pharmacy, Institute of Life Science and School of Public Health, 
      Chongqing Medical University, Chongqing 400016, PR China.
FAU - Tang, Zhiyong
AU  - Tang Z
AD  - College of Pharmacy, Institute of Life Science and School of Public Health, 
      Chongqing Medical University, Chongqing 400016, PR China.
FAU - Zhang, Chengli
AU  - Zhang C
AD  - College of Pharmacy, Institute of Life Science and School of Public Health, 
      Chongqing Medical University, Chongqing 400016, PR China.
FAU - Chen, Jun
AU  - Chen J
AD  - College of Pharmacy, Institute of Life Science and School of Public Health, 
      Chongqing Medical University, Chongqing 400016, PR China.
FAU - Li, Jia
AU  - Li J
AD  - College of Pharmacy, Institute of Life Science and School of Public Health, 
      Chongqing Medical University, Chongqing 400016, PR China.
FAU - Yu, Chao
AU  - Yu C
AD  - College of Pharmacy, Institute of Life Science and School of Public Health, 
      Chongqing Medical University, Chongqing 400016, PR China. Electronic address: 
      yuchao@cqmu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190219
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Antibodies, Immobilized)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 49DFR088MY (Platinum)
RN  - 7440-57-5 (Gold)
RN  - 7782-42-5 (Graphite)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Antibodies, Immobilized/chemistry/isolation & purification
MH  - *Biosensing Techniques
MH  - Chemokine CCL2/chemistry/*isolation & purification
MH  - *Electrochemical Techniques
MH  - Gold/chemistry
MH  - Graphite/chemistry
MH  - Humans
MH  - Hydrogen Peroxide/chemistry
MH  - Limit of Detection
MH  - Metal Nanoparticles/*chemistry
MH  - Nanocomposites/chemistry
MH  - Platinum/chemistry
OTO - NOTNLM
OT  - Immunosensor
OT  - Monocyte chemoattractant protein-1
OT  - Tetraethylene
OT  - Thionine
OT  - Trimetallic nanoalloy particles
EDAT- 2019/03/04 06:00
MHDA- 2019/07/11 06:00
CRDT- 2019/03/04 06:00
PHST- 2018/10/28 00:00 [received]
PHST- 2018/12/10 00:00 [revised]
PHST- 2019/02/05 00:00 [accepted]
PHST- 2019/03/04 06:00 [pubmed]
PHST- 2019/07/11 06:00 [medline]
PHST- 2019/03/04 06:00 [entrez]
AID - S0956-5663(19)30124-1 [pii]
AID - 10.1016/j.bios.2019.02.021 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2019 Apr 15;131:67-73. doi: 10.1016/j.bios.2019.02.021. Epub 
      2019 Feb 19.