PMID- 30827397
OWN - NLM
STAT- MEDLINE
DCOM- 20190419
LR  - 20190419
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 230
DP  - 2019 Mar
TI  - Assessment of pulmonary tissue responses in pigs challenged with PRRSV Lena 
      strain shows better protection after immunization with field than vaccine 
      strains.
PG  - 249-259
LID - S0378-1135(18)31074-5 [pii]
LID - 10.1016/j.vetmic.2019.01.022 [doi]
AB  - The porcine reproductive and respiratory syndrome virus (PRRSV) is plaguing 
      porcine production. Previously piglets were immunized with a PRRSV-1 commercial 
      modified live virus vaccine (MLV1), a PRRSV-2 MLV (MLV2) or a Western European 
      strain (Finistere: Fini) to assess and compare the protection brought by these 
      strains upon challenge with virulent Lena strain. Lena viremia was reduced in all 
      the immunized groups with a slightly higher level of protection following 
      immunization with Fini. Using lung samples collected from the same experiment, 
      tissue response to Lena challenge was assessed at the acute and chronic stages of 
      infection. A pre-immunization with any one of the three PRRSV strains globally 
      exacerbated microscopic lung lesions. Ten days post-challenge (DPC), MLV1 group 
      score was higher than unimmunized group score and 42 DPC, MLV2 group score was 
      higher than in unimmunized group. Lowest lung Lena viral loads were measured in 
      Fini group. Using principal component analysis, we showed 10 DPC that the lesion 
      score was positively correlated with chemokine receptors and negatively 
      correlated with viral load. Forty-two DPC, variables for lesion score, IL6, IL8, 
      and CCL20 transcripts were positively correlated together and negatively 
      correlated with CCL28, CXCL6, and CXCR4 transcripts suggesting a role for the 
      latter ones in the tissue recovery process. In conclusions, our study shows a 
      significant impact of the three immunizations on pulmonary tissue with the best 
      protection against Lena challenge conferred by Fini strain. Furthermore, it gives 
      insight into the interactions between vaccine and Fini strains and the lung upon 
      Lena challenge.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Larcher, Thibaut
AU  - Larcher T
AD  - PAnTher, INRA, Oniris, Universite Bretagne Loire, 44307, Nantes, France.
FAU - Fablet, Christelle
AU  - Fablet C
AD  - Agence Nationale de Securite Sanitaire Alimentation Environnement Travail 
      (Anses), BP 53, 22440, Ploufragan, France; Universite Bretagne Loire, 44307, 
      Nantes, France.
FAU - Renson, Patricia
AU  - Renson P
AD  - Agence Nationale de Securite Sanitaire Alimentation Environnement Travail 
      (Anses), BP 53, 22440, Ploufragan, France; Universite Bretagne Loire, 44307, 
      Nantes, France; IFIP-Institut du porc, 5 Rue Lespagnol, 75020, Paris, France.
FAU - Menard, Deborah
AU  - Menard D
AD  - BIOEPAR, INRA, Oniris, Universite Bretagne Loire, 44307, Nantes, France.
FAU - Hervet, Caroline
AU  - Hervet C
AD  - BIOEPAR, INRA, Oniris, Universite Bretagne Loire, 44307, Nantes, France.
FAU - Saade, Georges
AU  - Saade G
AD  - BIOEPAR, INRA, Oniris, Universite Bretagne Loire, 44307, Nantes, France.
FAU - Belloc, Catherine
AU  - Belloc C
AD  - BIOEPAR, INRA, Oniris, Universite Bretagne Loire, 44307, Nantes, France.
FAU - Bourry, Olivier
AU  - Bourry O
AD  - Agence Nationale de Securite Sanitaire Alimentation Environnement Travail 
      (Anses), BP 53, 22440, Ploufragan, France; Universite Bretagne Loire, 44307, 
      Nantes, France.
FAU - Meurens, Francois
AU  - Meurens F
AD  - BIOEPAR, INRA, Oniris, Universite Bretagne Loire, 44307, Nantes, France. 
      Electronic address: francois.meurens@oniris-nantes.fr.
LA  - eng
PT  - Journal Article
DEP - 20190214
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Viral)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*blood
MH  - Chemokines/immunology
MH  - Cytokines/immunology
MH  - Lung/immunology/pathology/*virology
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/*prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/genetics
MH  - Principal Component Analysis
MH  - Swine
MH  - Vaccines, Attenuated/immunology
MH  - Viral Load
MH  - Viral Vaccines/*immunology
MH  - Viremia
OTO - NOTNLM
OT  - Chemokine
OT  - Chemokine receptor
OT  - Genotype 1.3 strains
OT  - Immune response
OT  - Lung
OT  - PRRSV
OT  - Tissue scoring
EDAT- 2019/03/05 06:00
MHDA- 2019/04/20 06:00
CRDT- 2019/03/05 06:00
PHST- 2018/09/13 00:00 [received]
PHST- 2019/01/10 00:00 [revised]
PHST- 2019/01/27 00:00 [accepted]
PHST- 2019/03/05 06:00 [entrez]
PHST- 2019/03/05 06:00 [pubmed]
PHST- 2019/04/20 06:00 [medline]
AID - S0378-1135(18)31074-5 [pii]
AID - 10.1016/j.vetmic.2019.01.022 [doi]
PST - ppublish
SO  - Vet Microbiol. 2019 Mar;230:249-259. doi: 10.1016/j.vetmic.2019.01.022. Epub 2019 
      Feb 14.