PMID- 30830942
OWN - NLM
STAT- MEDLINE
DCOM- 20191202
LR  - 20200309
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 14
IP  - 3
DP  - 2019
TI  - Hydroalcoholic extract from Origanum vulgare induces a combined 
      anti-mycobacterial and anti-inflammatory response in innate immune cells.
PG  - e0213150
LID - 10.1371/journal.pone.0213150 [doi]
LID - e0213150
AB  - In nature, many plants or their extracted compounds have been found to possess 
      anti-inflammatory features and therapeutic properties against infectious as well 
      as non-infectious diseases, including cancer. In this study, we analysed the 
      immunomodulatory effects on innate immune cells of hydroalcoholic extract from 
      Origanum vulgare L. ssp. hirtum (HyE-Ov), a plant traditionally known for its 
      anti-oxidative properties. The effects of HyE-Ov were tested on human monocyte 
      derived dendritic cells (DC), type-1 (M1) and type-2 macrophages (M2) infected 
      with M. bovis Bacille Calmette-Guerin (BCG), used as a model of persistent 
      intracellular bacterium. DC, M1 and M2 treated with HyE-Ov significantly enhanced 
      their mycobactericidal activity, which was associated with phagosomal 
      acidification in M1 and M2 and increase of phagosomal, but not mitochondrial ROS 
      production in M1, M2, and DC. Treatment of BCG-infected DC with HyE-Ov 
      significantly reduced TNF-alpha and IL-12 production and increased TGF-beta synthesis. 
      Finally, experiments were repeated using eight different HPLC fractions of 
      HyE-Ov. Results showed that the capability to activate anti-microbial and 
      anti-inflammatory response is shared by different fractions, suggesting that 
      diverse bioactive molecules are present within the hydroalcoholic extract. 
      Altogether, these results show that HyE-Ov promotes anti-mycobacterial innate 
      immunity and limits inflammatory response in vitro and suggest that this plant 
      extract may be exploitable as phytocomplex or nutraceutical for novel 
      host-directed therapeutic approaches.
FAU - De Santis, Federica
AU  - De Santis F
AD  - Department of Biology, University of Rome "Tor Vergata", Rome, Italy.
FAU - Poerio, Noemi
AU  - Poerio N
AD  - Department of Biology, University of Rome "Tor Vergata", Rome, Italy.
FAU - Gismondi, Angelo
AU  - Gismondi A
AD  - Department of Biology, University of Rome "Tor Vergata", Rome, Italy.
FAU - Nanni, Valentina
AU  - Nanni V
AD  - Department of Biology, University of Rome "Tor Vergata", Rome, Italy.
FAU - Di Marco, Gabriele
AU  - Di Marco G
AD  - Department of Biology, University of Rome "Tor Vergata", Rome, Italy.
FAU - Nisini, Roberto
AU  - Nisini R
AD  - Department of infectious diseases, Istituto Superiore di Sanita, Rome, Italy.
FAU - Thaller, Maria Cristina
AU  - Thaller MC
AD  - Department of Biology, University of Rome "Tor Vergata", Rome, Italy.
FAU - Canini, Antonella
AU  - Canini A
AD  - Department of Biology, University of Rome "Tor Vergata", Rome, Italy.
FAU - Fraziano, Maurizio
AU  - Fraziano M
AUID- ORCID: 0000-0002-5643-0769
AD  - Department of Biology, University of Rome "Tor Vergata", Rome, Italy.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190304
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Alcohols)
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (IL2 protein, human)
RN  - 0 (Interleukin-2)
RN  - 0 (Plant Extracts)
RN  - 0 (TNF protein, human)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Alcohols/chemistry/*pharmacology
MH  - Anti-Infective Agents/chemistry/*pharmacology
MH  - Anti-Inflammatory Agents/chemistry/*pharmacology
MH  - Cells, Cultured
MH  - Dendritic Cells/cytology/*drug effects/immunology/microbiology
MH  - Healthy Volunteers
MH  - Humans
MH  - Immunity, Innate/drug effects
MH  - Interleukin-2/metabolism
MH  - Macrophages/cytology/*drug effects/immunology/microbiology
MH  - Mycobacterium bovis/*drug effects/pathogenicity
MH  - Origanum/*chemistry
MH  - Phagosomes/metabolism
MH  - Plant Extracts/chemistry/pharmacology
MH  - Transforming Growth Factor beta/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
PMC - PMC6398838
COIS- The authors have declared that no competing interests exist.
EDAT- 2019/03/05 06:00
MHDA- 2019/12/04 06:00
PMCR- 2019/03/04
CRDT- 2019/03/05 06:00
PHST- 2018/11/08 00:00 [received]
PHST- 2019/02/17 00:00 [accepted]
PHST- 2019/03/05 06:00 [entrez]
PHST- 2019/03/05 06:00 [pubmed]
PHST- 2019/12/04 06:00 [medline]
PHST- 2019/03/04 00:00 [pmc-release]
AID - PONE-D-18-32196 [pii]
AID - 10.1371/journal.pone.0213150 [doi]
PST - epublish
SO  - PLoS One. 2019 Mar 4;14(3):e0213150. doi: 10.1371/journal.pone.0213150. 
      eCollection 2019.