PMID- 30838616
OWN - NLM
STAT- MEDLINE
DCOM- 20190712
LR  - 20201209
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1943
DP  - 2019
TI  - Layer-By-Layer Film Engineering for Sequential Gene Delivery.
PG  - 161-176
LID - 10.1007/978-1-4939-9092-4_11 [doi]
AB  - Layer-by-layer (LbL) films are assembled with poly(amido amine)s (PAAs), a type 
      of polycations containing bioreducible disulfide bond, and DNA plasmids to enable 
      LbL film degradation in physiologic conditions by reacting with glutathione or 
      redox-active membrane proteins. The interior layer structure of the LbL films 
      during assembly and disassembly is studied by atomic force microscopy (AFM), 
      ellipsometry, dynamic light scattering (DLS), and fluorescence spectroscopy. 
      Insertion of barrier layers in bioreducible LbL films is necessary to stabilize 
      the interior layer structure and slow down the film degradation rate to achieve 
      sequential gene delivery. Localized gene delivery from the LbL films is 
      demonstrated using human embryonic kidney 293 (HEK 293) cells.
FAU - Xie, Lingxiao
AU  - Xie L
AD  - Department of Chemical Engineering and Materials Science, Wayne State University, 
      Detroit, MI, USA.
FAU - Zou, Yi
AU  - Zou Y
AD  - Department of Chemical Engineering and Materials Science, Wayne State University, 
      Detroit, MI, USA.
FAU - Carroll, Sean
AU  - Carroll S
AD  - Department of Chemical Engineering and Materials Science, Wayne State University, 
      Detroit, MI, USA.
AD  - Department of Biomedical Engineering, Wayne State University, Detroit, MI, USA.
FAU - Muniz, Maria
AU  - Muniz M
AD  - Department of Biomedical Engineering, Wayne State University, Detroit, MI, USA.
FAU - Mao, Guangzhao
AU  - Mao G
AD  - Department of Chemical Engineering and Materials Science, Wayne State University, 
      Detroit, MI, USA. guangzhao.mao@wayne.edu.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Poly(amidoamine))
RN  - 0 (Polyamines)
RN  - 0 (Polyelectrolytes)
RN  - 0 (polycations)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - DNA/genetics
MH  - Genetic Therapy/methods
MH  - HEK293 Cells
MH  - Humans
MH  - Nanoparticles/chemistry
MH  - Plasmids/genetics
MH  - Polyamines/*chemistry
MH  - Polyelectrolytes
MH  - Transfection/*methods
OTO - NOTNLM
OT  - Atomic force microscopy
OT  - Bioreducible poly(amido amine)
OT  - DNA delivery
OT  - Film degradation
OT  - Layer-by-layer (LbL) film
OT  - Polyelectrolyte multilayers
OT  - Sequential gene delivery
EDAT- 2019/03/07 06:00
MHDA- 2019/07/13 06:00
CRDT- 2019/03/07 06:00
PHST- 2019/03/07 06:00 [entrez]
PHST- 2019/03/07 06:00 [pubmed]
PHST- 2019/07/13 06:00 [medline]
AID - 10.1007/978-1-4939-9092-4_11 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2019;1943:161-176. doi: 10.1007/978-1-4939-9092-4_11.