PMID- 30840066
OWN - NLM
STAT- MEDLINE
DCOM- 20200706
LR  - 20200706
IS  - 1460-2431 (Electronic)
IS  - 0022-0957 (Print)
IS  - 0022-0957 (Linking)
VI  - 70
IP  - 10
DP  - 2019 May 9
TI  - Cit1,2RhaT and two novel CitdGlcTs participate in flavor-related flavonoid 
      metabolism during citrus fruit development.
PG  - 2759-2771
LID - 10.1093/jxb/erz081 [doi]
AB  - Neohesperidosides are disaccharides that are present in some flavonoids and 
      impart a bitter taste, which can significantly affect the commercial value of 
      citrus fruits. In this study, we identified three 
      flavonoid-7-O-di-glucosyltransferase (dGlcT) genes closely related to 
      1,2-rhamnosyltransferase (1,2RhaT) in citrus genomes. However, only 1,2RhaT was 
      directly linked to the accumulation of neohesperidoside, as demonstrated by 
      association analysis of 50 accessions and co-segregation analysis of an F1 
      population derived from Citrus reticulata x Poncirus trifoliata. In transgenic 
      tobacco BY2 cells, over-expression of CitdGlcTs resulted in 
      flavonoid-7-O-glucosides being catalysed into bitterless 
      flavonoid-7-O-di-glucosides, whereas over-expression of Cit1,2RhaT converted the 
      same substrate into bitter-tasting flavonoid-7-O-neohesperidoside. Unlike 
      1,2RhaT, during citrus fruit development the dGlcTs showed an opposite expression 
      pattern to CHS and CHI, two genes encoding rate-limiting enzymes of flavonoid 
      biosynthesis. An uncoupled availability of dGlcTs and substrates might result in 
      trace accumulation of flavonoid-7-O-di-glucosides in the fruit of C. maxima 
      (pummelo). Past human selection of the deletion and functional mutation of 
      1,2RhaT has led step-by-step to the evolution of the flavor-related metabolic 
      network in citrus. Our research provides the basis for potentially improving the 
      taste in citrus fruit through manipulation of the network by knocking-out 1,2RhaT 
      or by enhancing the expression of dGlcT using genetic transformation.
CI  - (c) The Author(s) 2019. Published by Oxford University Press on behalf of the 
      Society for Experimental Biology.
FAU - Chen, Jiajing
AU  - Chen J
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, Wuhan, PR China.
FAU - Yuan, Ziyu
AU  - Yuan Z
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, Wuhan, PR China.
FAU - Zhang, Haipeng
AU  - Zhang H
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, Wuhan, PR China.
FAU - Li, Wenyun
AU  - Li W
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, Wuhan, PR China.
AD  - Guizhou Fruit Institute, Guizhou Academy of Agricultural Sciences, Guiyang, 
      Guizhou Province, China.
FAU - Shi, Meiyan
AU  - Shi M
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, Wuhan, PR China.
FAU - Peng, Zhaoxin
AU  - Peng Z
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, Wuhan, PR China.
FAU - Li, Mingyue
AU  - Li M
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, Wuhan, PR China.
FAU - Tian, Jing
AU  - Tian J
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, Wuhan, PR China.
FAU - Deng, Xiuxin
AU  - Deng X
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, Wuhan, PR China.
FAU - Cheng, Yunjiang
AU  - Cheng Y
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, Wuhan, PR China.
FAU - Deng, Cecilia Hong
AU  - Deng CH
AD  - The New Zealand Institute for Plant & Food Research Limited, Private Bag, 
      Auckland, New Zealand.
FAU - Xie, Zongzhou
AU  - Xie Z
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, Wuhan, PR China.
FAU - Zeng, Jiwu
AU  - Zeng J
AD  - Guangdong Fruit Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 
      Guangdong Province, China.
FAU - Yao, Jia-Long
AU  - Yao JL
AD  - The New Zealand Institute for Plant & Food Research Limited, Private Bag, 
      Auckland, New Zealand.
FAU - Xu, Juan
AU  - Xu J
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, Wuhan, PR China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Exp Bot
JT  - Journal of experimental botany
JID - 9882906
RN  - 0 (Flavonoids)
SB  - IM
MH  - Citrus/enzymology/growth & development/*metabolism
MH  - Flavonoids/*metabolism
MH  - Fruit/growth & development/*metabolism
MH  - Genes, Plant
MH  - Hybridization, Genetic
MH  - Poncirus/enzymology/growth & development/*metabolism
PMC - PMC6506761
OTO - NOTNLM
OT  - Bitterness
OT  - citrus
OT  - flavonoid
OT  - flavonoid-7-O-di-glucosides
OT  - flavonoid-7-O-glucoside
OT  - neohesperidoside
EDAT- 2019/03/07 06:00
MHDA- 2020/07/07 06:00
PMCR- 2019/03/06
CRDT- 2019/03/07 06:00
PHST- 2018/09/19 00:00 [received]
PHST- 2019/02/14 00:00 [accepted]
PHST- 2019/03/07 06:00 [pubmed]
PHST- 2020/07/07 06:00 [medline]
PHST- 2019/03/07 06:00 [entrez]
PHST- 2019/03/06 00:00 [pmc-release]
AID - 5370300 [pii]
AID - erz081 [pii]
AID - 10.1093/jxb/erz081 [doi]
PST - ppublish
SO  - J Exp Bot. 2019 May 9;70(10):2759-2771. doi: 10.1093/jxb/erz081.