PMID- 30840800 OWN - NLM STAT- MEDLINE DCOM- 20190412 LR - 20200225 IS - 1738-0006 (Electronic) IS - 0023-4001 (Print) IS - 0023-4001 (Linking) VI - 57 IP - 1 DP - 2019 Feb TI - Effect of Temperature on the Killing of Opisthorchis viverrini Eggs In Vitro. PG - 49-53 LID - 10.3347/kjp.2019.57.1.49 [doi] AB - Contaminated liver fluke egg in the environment has led to the high prevalence of human opisthorchiasis associated with cholangiocarcinoma in Southeast Asia. To find the effective lessening methods of Opisthorchis viverrini eggs in the contaminated environment, we investigated the temperature conditions for killing of these trematode eggs in vitro. Numerous O. viverrini eggs were obtained in the proximal part of uteri of adult worms from experimental hamsters. Mature eggs with miracidium were allocated by experimental groups (2 control: positive and negative and 4 treatment: 50, 60, 70, and 80 degrees C) with 0.85% saline, and treated by the experimental plan. Eggs in each experimental groups were observed under the confocal microscope after stain with Propidium Iodide (PI) to evaluate the effect of temperatures. Eggs in 70 and 80 degrees C groups were all killed after over 10 min heated. Majority of eggs in 60 degrees C (10, 15, and 30 min heated), 70 and 80 degrees C (5 min heated) groups were inactivated. However in 50 degrees C group, below half of eggs were to be killed in all time lapse (10, 15 and 30 min). In order to prevent O. viverrini infection and cholangiocarcinoma, direct treatment of sewage by heating at 70 or 80 degrees C at least 10 min is essential. Therefore, treatment of O. viverrini eggs at a high temperature is a potential method for controlling egg contamination in sewage. FAU - Boueroy, Parichart AU - Boueroy P AD - Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. AD - Neglected, zoonosis, and vector-borne disease group, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. AD - Liver Fluke and Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand. FAU - Duenngai, Kunyarat AU - Duenngai K AD - Liver Fluke and Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand. AD - Department of Public Health, Faculty of Science and Technology, Phetchabun Rajabhat University, Phetchabun 67000, Thailand. FAU - Eamudomkarn, Chatanun AU - Eamudomkarn C AD - Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. FAU - Sripan, Panupan AU - Sripan P AD - Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. AD - Neglected, zoonosis, and vector-borne disease group, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. AD - Liver Fluke and Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand. FAU - Boonmars, Thidarut AU - Boonmars T AD - Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. AD - Neglected, zoonosis, and vector-borne disease group, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. AD - Liver Fluke and Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand. FAU - Pumhirunroj, Benjamabhorn AU - Pumhirunroj B AD - Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. AD - Neglected, zoonosis, and vector-borne disease group, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. AD - Liver Fluke and Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand. FAU - Artchayasawat, Atchara AU - Artchayasawat A AD - Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. AD - Neglected, zoonosis, and vector-borne disease group, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. FAU - Songsri, Jiraporn AU - Songsri J AD - Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. AD - Neglected, zoonosis, and vector-borne disease group, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. AD - Liver Fluke and Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand. FAU - Chomphumee, Kanpicha AU - Chomphumee K AD - Liver Fluke and Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand. FAU - Rattanasuwan, Panaratana AU - Rattanasuwan P AD - Department of Anesthesiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. FAU - Laummaunwai, Porntip AU - Laummaunwai P AD - Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. AD - Neglected, zoonosis, and vector-borne disease group, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. FAU - Khueangchiangkhwang, Sukhonthip AU - Khueangchiangkhwang S AD - Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. AD - Neglected, zoonosis, and vector-borne disease group, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. AD - Liver Fluke and Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand. FAU - Boonjaraspinyo, Sirintip AU - Boonjaraspinyo S AD - Department of Community Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. LA - eng GR - 05/2560/Central Avian Research Institute/ GR - RTA5580004/Thailand Research Fund/ GR - 58441/Khon Kaen University/ PT - Journal Article DEP - 20190226 PL - Korea (South) TA - Korean J Parasitol JT - The Korean journal of parasitology JID - 9435800 RN - 0 (Fluorescent Dyes) RN - 36015-30-2 (Propidium) SB - IM MH - Animals MH - Cricetinae MH - Fluorescent Dyes/metabolism MH - *Hot Temperature MH - Microscopy, Confocal MH - Opisthorchis/*physiology/*radiation effects MH - Propidium/metabolism MH - Staining and Labeling MH - Survival Analysis MH - Zygote/*physiology/*radiation effects PMC - PMC6409225 OTO - NOTNLM OT - Opisthorchis viverrini OT - egg OT - heating OT - temperature OT - treatment COIS- CONFLICT OF INTEREST We have no conflict of interest related to this work. EDAT- 2019/03/07 06:00 MHDA- 2019/04/13 06:00 PMCR- 2019/02/01 CRDT- 2019/03/07 06:00 PHST- 2018/07/28 00:00 [received] PHST- 2019/01/13 00:00 [accepted] PHST- 2019/03/07 06:00 [entrez] PHST- 2019/03/07 06:00 [pubmed] PHST- 2019/04/13 06:00 [medline] PHST- 2019/02/01 00:00 [pmc-release] AID - kjp.2019.57.1.49 [pii] AID - kjp-57-1-49 [pii] AID - 10.3347/kjp.2019.57.1.49 [doi] PST - ppublish SO - Korean J Parasitol. 2019 Feb;57(1):49-53. doi: 10.3347/kjp.2019.57.1.49. Epub 2019 Feb 26.