PMID- 30840970 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20201001 IS - 2005-3711 (Print) IS - 1598-7876 (Electronic) IS - 1225-8245 (Linking) VI - 62 IP - 2 DP - 2019 Mar TI - Reduction of Inflammation and Enhancement of Motility after Pancreatic Islet Derived Stem Cell Transplantation Following Spinal Cord Injury. PG - 153-165 LID - 10.3340/jkns.2018.0035 [doi] AB - OBJECTIVE: Spinal cord injury (SCI) is a very serious health problem, usually caused by a trauma and accompanied by elevated levels of inflammation indicators. Stem cell-based therapy is promising some valuable strategies for its functional recovery. Nestinpositive progenitor and/or stem cells (SC) isolated from pancreatic islets (PI) show mesenchymal stem cell (MSC) characteristics. For this reason, we aimed to analyze the effects of rat pancreatic islet derived stem cell (rPI-SC) delivery on functional recovery, as well as the levels of inflammation factors following SCI. METHODS: rPI-SCs were isolated, cultured and their MSC characteristics were determined through flow cytometry and immunofluorescence analysis. The experimental rat population was divided into three groups : 1) laminectomy & trauma, 2) laminectomy & trauma & phosphate-buffered saline (PBS), and 3) laminectomy+trauma+SCs. Green fluorescent protein (GFP) labelled rPI-SCs were transplanted into the injured rat spinal cord. Their motilities were evaluated with Basso, Beattie and Bresnahan (BBB) Score. After 4-weeks, spinal cord sections were analyzed for GFP labeled SCs and stained for vimentin, S100beta, brain derived neurotrophic factor (BDNF), 2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNPase), vascular endothelial growth factor (VEGF) and proinflammatory (interleukin [IL]-6, transforming growth factor [TGF]-beta, macrophage inflammatory protein [MIP]-2, myeloperoxidase [MPO]) and anti-inflammatory (IL-1 receptor antagonis) factors. RESULTS: rPI-SCs were revealed to display MSC characteristics and express neural and glial cell markers including BDNF, glial fibrillary acidic protein (GFAP), fibronectin, microtubule associated protein-2a,b (MAP2a,b), beta3-tubulin and nestin as well as antiinflammatory prostaglandin E2 receptor, EP3. The BBB scores showed significant motor recovery in group 3. GFP-labelled cells were localized on the injury site. In addition, decreased proinflammatory factor levels and increased intensity of anti-inflammatory factors were determined. CONCLUSION: Transplantation of PI-SCs might be an effective strategy to improve functional recovery following spinal cord trauma. FAU - Karaoz, Erdal AU - Karaoz E AD - Department of Histology & Embryology, Faculty of Medicine, Istinye University, Istanbul, Turkey. AD - Center for Stem Cell and Tissue Engineering Research & Practice, Istinye University, Istanbul, Turkey. AD - Center for Regenerative Medicine and Stem Cell Research & Manufacturing (LivMedCell), Istanbul, Turkey. FAU - Tepekoy, Filiz AU - Tepekoy F AD - Department of Histology & Embryology, Faculty of Medicine, Istinye University, Istanbul, Turkey. FAU - Yilmaz, Irem AU - Yilmaz I AD - Center for Regenerative Medicine and Stem Cell Research & Manufacturing (LivMedCell), Istanbul, Turkey. FAU - Subasi, Cansu AU - Subasi C AD - Center for Regenerative Medicine and Stem Cell Research & Manufacturing (LivMedCell), Istanbul, Turkey. FAU - Kabatas, Serdar AU - Kabatas S AD - Neurosurgery Clinic, Gaziosmanpasa Taksim Training and Research Hospital, Istanbul, Turkey. LA - eng PT - Journal Article DEP - 20190227 PL - Korea (South) TA - J Korean Neurosurg Soc JT - Journal of Korean Neurosurgical Society JID - 101467054 PMC - PMC6411578 OTO - NOTNLM OT - Islets of langerhans OT - Regeneration OT - Spinal cord OT - Stem cells OT - Wounds and injuries COIS- No potential conflict of interest relevant to this article was reported. EDAT- 2019/03/07 06:00 MHDA- 2019/03/07 06:01 PMCR- 2019/03/01 CRDT- 2019/03/07 06:00 PHST- 2018/02/09 00:00 [received] PHST- 2018/06/23 00:00 [accepted] PHST- 2019/03/07 06:00 [entrez] PHST- 2019/03/07 06:00 [pubmed] PHST- 2019/03/07 06:01 [medline] PHST- 2019/03/01 00:00 [pmc-release] AID - jkns.2018.0035 [pii] AID - jkns-2018-0035 [pii] AID - 10.3340/jkns.2018.0035 [doi] PST - ppublish SO - J Korean Neurosurg Soc. 2019 Mar;62(2):153-165. doi: 10.3340/jkns.2018.0035. Epub 2019 Feb 27.