PMID- 30841605 OWN - NLM STAT- MEDLINE DCOM- 20190508 LR - 20200225 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 11 IP - 3 DP - 2019 Mar 5 TI - Dianthus superbus Improves Glomerular Fibrosis and Renal Dysfunction in Diabetic Nephropathy Model. LID - 10.3390/nu11030553 [doi] LID - 553 AB - Glomerular fibrosis is caused by an accumulation of intercellular spaces containing mesangial matrix proteins through either diffused or nodular changes. Dianthus superbus has been used in traditional medicine as a diuretic, a contraceptive, and an anti-inflammatory agent. The aim of this study was to investigate the effects of Dianthus superbus-EtOAc soluble fraction (DS-EA) on glomerular fibrosis and renal dysfunction, which has been implicated in diabetic nephropathy in human renal mesangial cells and db/db mice. DS-EA was administered to db/db mice at 10 or 50 mg/kg/day for 8 weeks. DS-EA treatment significantly ameliorated blood glucose, insulin, the homeostasis model assessment of insulin resistance (HOMA-IR) index, and HbA1c in diabetic mice. DS-EA decreased albumin excretion, creatinine clearance (Ccr), and plasma creatinine levels. DS-EA also ameliorated the levels of kidney injury molecules-1 (KIM-1) and C-reactive protein. DS-EA reduced the periodic acid-Schiff (PAS) staining intensity and basement membrane thickening in glomeruli of the diabetic nephropathy model. In addition, DS-EA suppressed transforming growth factor-beta (TGF-beta)/Smad signaling. Collagen type IV, a glomerular fibrosis biomarker, was significantly decreased upon DS-EA administration. DS-EA pretreatment attenuated levels of inflammation factors such as intracellular cell adhesion molecule-1 (ICAM-1) and monocyte chemoattractant protein-1 (MCP-1). DS-EA inhibited the translocation of nuclear factor kappa B (NF-kappaB) in Angiotensin II (Ang II)-stimulated mesangial cells. These findings suggest that DS-EA has a protective effect against renal inflammation and fibrosis. Therefore, DS-EA may serve as a potential therapeutic agent targeting glomerulonephritis and glomerulosclerosis, which lead to diabetic nephropathy. FAU - Yoon, Jung Joo AU - Yoon JJ AD - Hanbang Cardio-Renal Syndrome Research Center, Wonkwang University, 460, Iksan-daero, Iksan, Jeonbuk 54538, Korea. mora16@naver.com. AD - College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, 460, Iksan-daero, Iksan, Jeonbuk 54538, Korea. mora16@naver.com. FAU - Park, Ji Hun AU - Park JH AD - College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, 460, Iksan-daero, Iksan, Jeonbuk 54538, Korea. jihuncjstk@naver.com. FAU - Kim, Hye Jin AU - Kim HJ AD - College of Pharmacy, Wonkwang University, Iksan 54538, Korea. mn1003@naver.com. FAU - Jin, Hong-Guang AU - Jin HG AD - College of Pharmacy, Wonkwang University, Iksan 54538, Korea. hg_jin1979@163.com. AD - School of Pharmacy and Life Sciences, Jiujiang University, Jiujiang 332005, China. hg_jin1979@163.com. FAU - Kim, Hye Yoom AU - Kim HY AD - Hanbang Cardio-Renal Syndrome Research Center, Wonkwang University, 460, Iksan-daero, Iksan, Jeonbuk 54538, Korea. hyeyoomc@naver.com. AD - College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, 460, Iksan-daero, Iksan, Jeonbuk 54538, Korea. hyeyoomc@naver.com. FAU - Ahn, You Mee AU - Ahn YM AD - Hanbang Cardio-Renal Syndrome Research Center, Wonkwang University, 460, Iksan-daero, Iksan, Jeonbuk 54538, Korea. aum2668@naver.com. AD - College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, 460, Iksan-daero, Iksan, Jeonbuk 54538, Korea. aum2668@naver.com. FAU - Kim, Youn Chul AU - Kim YC AD - College of Pharmacy, Wonkwang University, Iksan 54538, Korea. yckim@wku.ac.kr. FAU - Lee, Ho Sub AU - Lee HS AD - Hanbang Cardio-Renal Syndrome Research Center, Wonkwang University, 460, Iksan-daero, Iksan, Jeonbuk 54538, Korea. host@wku.ac.kr. AD - College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, 460, Iksan-daero, Iksan, Jeonbuk 54538, Korea. host@wku.ac.kr. FAU - Lee, Yun Jung AU - Lee YJ AUID- ORCID: 0000-0002-9347-8606 AD - Hanbang Cardio-Renal Syndrome Research Center, Wonkwang University, 460, Iksan-daero, Iksan, Jeonbuk 54538, Korea. shrons@wku.ac.kr. AD - College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, 460, Iksan-daero, Iksan, Jeonbuk 54538, Korea. shrons@wku.ac.kr. FAU - Kang, Dae Gill AU - Kang DG AD - Hanbang Cardio-Renal Syndrome Research Center, Wonkwang University, 460, Iksan-daero, Iksan, Jeonbuk 54538, Korea. dgkang@wku.ac.kr. AD - College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, 460, Iksan-daero, Iksan, Jeonbuk 54538, Korea. dgkang@wku.ac.kr. LA - eng GR - 2017R1A5A2015805/Ministry of Science ICT and Future Planning/ GR - 2015M3A9A5030620/Ministry of Science ICT and Future Planning/ GR - 2018R1D1A1B07050720/Ministry of Science ICT and Future Planning/ PT - Journal Article DEP - 20190305 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 0 (Blood Glucose) RN - 0 (Chemokine CCL2) RN - 0 (Collagen Type IV) RN - 0 (Insulin) RN - 0 (Plant Extracts) RN - 0 (Protective Agents) RN - 0 (Transforming Growth Factor beta) RN - 126547-89-5 (Intercellular Adhesion Molecule-1) SB - IM EIN - Nutrients. 2020 Jun 03;12(6):. PMID: 32503102 MH - Animals MH - Blood Glucose/drug effects MH - Chemokine CCL2/blood MH - Collagen Type IV/blood MH - Diabetes Mellitus, Experimental/complications MH - Diabetic Nephropathies/blood/*drug therapy/etiology MH - *Dianthus MH - Disease Models, Animal MH - Fibrosis MH - Humans MH - Inflammation MH - Insulin/blood MH - Insulin Resistance/physiology MH - Intercellular Adhesion Molecule-1/blood MH - Kidney/drug effects/pathology/physiopathology MH - Mesangial Cells MH - Mice MH - *Phytotherapy MH - Plant Extracts/*pharmacology MH - Protective Agents/*pharmacology MH - Signal Transduction MH - Transforming Growth Factor beta/blood PMC - PMC6471502 OTO - NOTNLM OT - Dianthus superbus OT - db/db mice OT - diabetic nephropathy OT - fibrosis OT - inflammation OT - mesangial cell COIS- The authors declare no conflicts of interest. EDAT- 2019/03/08 06:00 MHDA- 2019/05/09 06:00 PMCR- 2019/03/01 CRDT- 2019/03/08 06:00 PHST- 2019/01/16 00:00 [received] PHST- 2019/02/22 00:00 [revised] PHST- 2019/02/28 00:00 [accepted] PHST- 2019/03/08 06:00 [entrez] PHST- 2019/03/08 06:00 [pubmed] PHST- 2019/05/09 06:00 [medline] PHST- 2019/03/01 00:00 [pmc-release] AID - nu11030553 [pii] AID - nutrients-11-00553 [pii] AID - 10.3390/nu11030553 [doi] PST - epublish SO - Nutrients. 2019 Mar 5;11(3):553. doi: 10.3390/nu11030553.