PMID- 30841651 OWN - NLM STAT- MEDLINE DCOM- 20190610 LR - 20231006 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 20 IP - 5 DP - 2019 Mar 5 TI - On a Cold Night: Transcriptomics of Grapevine Flower Unveils Signal Transduction and Impacted Metabolism. LID - 10.3390/ijms20051130 [doi] LID - 1130 AB - Low temperature is a critical environmental factor limiting plant productivity, especially in northern vineyards. To clarify the impact of this stress on grapevine flower, we used the Vitis array based on Roche-NimbleGen technology to investigate the gene expression of flowers submitted to a cold night. Our objectives were to identify modifications in the transcript levels after stress and during recovery. Consequently, our results confirmed some mechanisms known in grapes or other plants in response to cold stress, notably, (1) the pivotal role of calcium/calmodulin-mediated signaling; (2) the over-expression of sugar transporters and some genes involved in plant defense (especially in carbon metabolism), and (3) the down-regulation of genes encoding galactinol synthase (GOLS), pectate lyases, or polygalacturonases. We also identified some mechanisms not yet known to be involved in the response to cold stress, i.e., (1) the up-regulation of genes encoding G-type lectin S-receptor-like serine threonine-protein kinase, pathogen recognition receptor (PRR5), or heat-shock factors among others; (2) the down-regulation of Myeloblastosis (MYB)-related transcription factors and the Constans-like zinc finger family; and (3) the down-regulation of some genes encoding Pathogen-Related (PR)-proteins. Taken together, our results revealed interesting features and potentially valuable traits associated with stress responses in the grapevine flower. From a long-term perspective, our study provides useful starting points for future investigation. FAU - Sawicki, Melodie AU - Sawicki M AD - Unite de Recherche Resistance Induite et Bioprotection des Plantes-EA 4707, Universite de Reims Champagne-Ardenne, UFR Sciences Exactes et Naturelles, SFR Condorcet FR CNRS 3417, Moulin de la Housse-Batiment 18, BP 1039, 51687 REIMS Cedex 2, France. me.sawicki@laposte.net. FAU - Rondeau, Marine AU - Rondeau M AUID- ORCID: 0000-0001-6398-3735 AD - Unite de Recherche Resistance Induite et Bioprotection des Plantes-EA 4707, Universite de Reims Champagne-Ardenne, UFR Sciences Exactes et Naturelles, SFR Condorcet FR CNRS 3417, Moulin de la Housse-Batiment 18, BP 1039, 51687 REIMS Cedex 2, France. marine.rondeau@univ-reims.fr. FAU - Courteaux, Barbara AU - Courteaux B AD - Unite de Recherche Resistance Induite et Bioprotection des Plantes-EA 4707, Universite de Reims Champagne-Ardenne, UFR Sciences Exactes et Naturelles, SFR Condorcet FR CNRS 3417, Moulin de la Housse-Batiment 18, BP 1039, 51687 REIMS Cedex 2, France. barbara.courteaux@univ-reims.fr. FAU - Rabenoelina, Fanja AU - Rabenoelina F AD - Unite de Recherche Resistance Induite et Bioprotection des Plantes-EA 4707, Universite de Reims Champagne-Ardenne, UFR Sciences Exactes et Naturelles, SFR Condorcet FR CNRS 3417, Moulin de la Housse-Batiment 18, BP 1039, 51687 REIMS Cedex 2, France. clarisse.rabenoelina@univ-reims.fr. FAU - Guerriero, Gea AU - Guerriero G AUID- ORCID: 0000-0001-6586-6997 AD - Luxembourg Institute of Science and Technology (LIST), Environmental Research and Innovation (ERIN) Department, 41 rue du Brill, L- 4422 Belvaux, Luxembourg. gea.guerriero@list.lu. FAU - Gomes, Eric AU - Gomes E AUID- ORCID: 0000-0003-2582-1203 AD - Institute of Vine and Wine Sciences, UMR 1287 Ecophysiology and Grape Functional Genomics, University of Bordeaux, INRA 210 Chemin de Leysotte - CS 50008, 33882 Villenave d'Ornon CEDEX, France. eric.gomes@inra.fr. FAU - Soubigou-Taconnat, Ludivine AU - Soubigou-Taconnat L AD - Institute of Plant Sciences Paris Saclay IPS2, CNRS, INRA, Universite Paris-Sud, Universite Evry, Universite Paris-Saclay, Batiment 630, 91405 Orsay, France. ludivine.soubigou-taconnat@inra.fr. FAU - Balzergue, Sandrine AU - Balzergue S AD - Institute of Plant Sciences Paris Saclay IPS2, CNRS, INRA, Universite Paris-Sud, Universite Evry, Universite Paris-Saclay, Batiment 630, 91405 Orsay, France. sandrine.balzergue@inra.fr. AD - Institute of Plant Sciences Paris-Saclay IPS2, Paris Diderot, Sorbonne Paris-Cite, Batiment 630, 91405, Orsay, France. sandrine.balzergue@inra.fr. AD - IRHS, INRA, AGROCAMPUS-Ouest, Universite d'Angers, SFR 4207 QUASAV, 42 rue Georges Morel, 49071 Beaucouze CEDEX, France. sandrine.balzergue@inra.fr. FAU - Clement, Christophe AU - Clement C AD - Unite de Recherche Resistance Induite et Bioprotection des Plantes-EA 4707, Universite de Reims Champagne-Ardenne, UFR Sciences Exactes et Naturelles, SFR Condorcet FR CNRS 3417, Moulin de la Housse-Batiment 18, BP 1039, 51687 REIMS Cedex 2, France. christophe.clement@univ-reims.fr. FAU - Ait Barka, Essaid AU - Ait Barka E AUID- ORCID: 0000-0002-5138-306X AD - Unite de Recherche Resistance Induite et Bioprotection des Plantes-EA 4707, Universite de Reims Champagne-Ardenne, UFR Sciences Exactes et Naturelles, SFR Condorcet FR CNRS 3417, Moulin de la Housse-Batiment 18, BP 1039, 51687 REIMS Cedex 2, France. ea.barka@univ-reims.fr. FAU - Vaillant-Gaveau, Nathalie AU - Vaillant-Gaveau N AD - Unite de Recherche Resistance Induite et Bioprotection des Plantes-EA 4707, Universite de Reims Champagne-Ardenne, UFR Sciences Exactes et Naturelles, SFR Condorcet FR CNRS 3417, Moulin de la Housse-Batiment 18, BP 1039, 51687 REIMS Cedex 2, France. nathalie.vaillant-gaveau@univ-reims.fr. FAU - Jacquard, Cedric AU - Jacquard C AUID- ORCID: 0000-0003-4284-6082 AD - Unite de Recherche Resistance Induite et Bioprotection des Plantes-EA 4707, Universite de Reims Champagne-Ardenne, UFR Sciences Exactes et Naturelles, SFR Condorcet FR CNRS 3417, Moulin de la Housse-Batiment 18, BP 1039, 51687 REIMS Cedex 2, France. cedric.jacquard@univ-reims.fr. LA - eng GR - 2014-2017/Reims Metropole/ GR - 2010-2013/Conseil Regional Champagne Ardenne/ GR - 2010-2013/Conseil Interprofessionnel du Vin de Bordeaux/ PT - Journal Article DEP - 20190305 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - *Cold-Shock Response MH - Flowers/genetics MH - Gene Expression Regulation, Plant MH - Signal Transduction MH - *Transcriptome MH - Vitis/*genetics/metabolism PMC - PMC6429367 OTO - NOTNLM OT - Vitis vinifera OT - candidate genes expression OT - cell wall OT - cold stress OT - flower OT - secondary metabolism OT - signaling cascades COIS- The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results. EDAT- 2019/03/08 06:00 MHDA- 2019/06/14 06:00 PMCR- 2019/03/01 CRDT- 2019/03/08 06:00 PHST- 2019/02/11 00:00 [received] PHST- 2019/02/26 00:00 [revised] PHST- 2019/03/01 00:00 [accepted] PHST- 2019/03/08 06:00 [entrez] PHST- 2019/03/08 06:00 [pubmed] PHST- 2019/06/14 06:00 [medline] PHST- 2019/03/01 00:00 [pmc-release] AID - ijms20051130 [pii] AID - ijms-20-01130 [pii] AID - 10.3390/ijms20051130 [doi] PST - epublish SO - Int J Mol Sci. 2019 Mar 5;20(5):1130. doi: 10.3390/ijms20051130.