PMID- 30842206
OWN - NLM
STAT- MEDLINE
DCOM- 20191128
LR  - 20200309
IS  - 2160-1836 (Electronic)
IS  - 2160-1836 (Linking)
VI  - 9
IP  - 5
DP  - 2019 May 7
TI  - The Neurospora crassa Standard Oak Ridge Background Exhibits Atypically Efficient 
      Meiotic Silencing by Unpaired DNA.
PG  - 1487-1496
LID - 10.1534/g3.119.400006 [doi]
AB  - Meiotic silencing by unpaired DNA (MSUD), an RNAi-mediated gene silencing 
      process, is efficient in crosses made in the Neurospora crassa standard Oak Ridge 
      (OR) genetic background. However, MSUD was decidedly less efficient when the 
      OR-derived MSUD testers were crossed with many wild-isolated strains (W), 
      suggesting that either sequence heterozygosity in tester x W crosses suppresses 
      MSUD, or that OR represents the MSUD-conducive extreme in the range of genetic 
      variation in MSUD efficiency. Our results support the latter model. MSUD was less 
      efficient in near-isogenic crosses made in the novel N. crassa B/S1 genetic 
      background, and in N. tetrasperma strain 85. Possibly, in B/S1 and 85, additional 
      regulatory cues, absent from OR, calibrate the MSUD response. A locus in distal 
      chromosome 1R appears to underlie the OR vs. B/S1 difference. Repeat-induced 
      point mutation (RIP) destroys duplicated genes by G:C to A:T mutation of 
      duplicated DNA sequences. Chromosome segment duplications (Dps) dominantly 
      suppress RIP, possibly by titrating out the RIP machinery. In Dp x N crosses, the 
      Dp-borne genes cannot pair properly, hence efficient MSUD, as in OR, silences 
      them and renders the crosses barren. We speculate that the increased productivity 
      engendered by inefficient MSUD enables small duplications to escape RIP.
CI  - Copyright (c) 2019 Giri et al.
FAU - Giri, Dev Ashish
AU  - Giri DA
AD  - Centre for DNA Fingerprinting and Diagnostics, Hyderabad 500039, India.
AD  - Graduate Studies, Manipal University.
FAU - Pankajam, Ajith V
AU  - Pankajam AV
AD  - School of Biology.
FAU - Nishant, Koodali T
AU  - Nishant KT
AUID- ORCID: 0000-0002-4342-7501
AD  - School of Biology.
AD  - Centre for Computation Modelling and Simulation, Indian Institute of Science 
      Education and Research, Thiruvananthapuram 695551, India.
FAU - Kasbekar, Durgadas P
AU  - Kasbekar DP
AUID- ORCID: 0000-0002-5269-8020
AD  - Centre for DNA Fingerprinting and Diagnostics, Hyderabad 500039, India 
      kas@cdfd.org.in.
LA  - eng
SI  - figshare/10.6084/m9.figshare.7570934
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190507
PL  - England
TA  - G3 (Bethesda)
JT  - G3 (Bethesda, Md.)
JID - 101566598
RN  - 0 (DNA, Fungal)
SB  - IM
MH  - Crosses, Genetic
MH  - *DNA, Fungal
MH  - *Gene Silencing
MH  - Genes, Fungal
MH  - Genome, Fungal
MH  - Genomics/methods
MH  - Genotype
MH  - Meiosis/*genetics
MH  - Neurospora crassa/*genetics/physiology
PMC - PMC6505168
OTO - NOTNLM
OT  - RIP suppression
OT  - chromosome segment duplication
OT  - genome heterozygosity
OT  - introgression
EDAT- 2019/03/08 06:00
MHDA- 2019/11/30 06:00
PMCR- 2019/03/06
CRDT- 2019/03/08 06:00
PHST- 2019/03/08 06:00 [pubmed]
PHST- 2019/11/30 06:00 [medline]
PHST- 2019/03/08 06:00 [entrez]
PHST- 2019/03/06 00:00 [pmc-release]
AID - g3.119.400006 [pii]
AID - GGG_400006 [pii]
AID - 10.1534/g3.119.400006 [doi]
PST - epublish
SO  - G3 (Bethesda). 2019 May 7;9(5):1487-1496. doi: 10.1534/g3.119.400006.