PMID- 30842368
OWN - NLM
STAT- MEDLINE
DCOM- 20190328
LR  - 20190328
IS  - 1880-3989 (Electronic)
IS  - 0388-1350 (Linking)
VI  - 44
IP  - 3
DP  - 2019
TI  - Mechanisms of silver nanoparticles-induced cytotoxicity and apoptosis in rat 
      tracheal epithelial cells.
PG  - 155-165
LID - 10.2131/jts.44.155 [doi]
AB  - Silver nanoparticles (AgNPs) are increasingly utilized in a number of 
      applications. This study was designed to investigate AgNPs induced cytotoxicity, 
      oxidative stress and apoptosis in rat tracheal epithelial cells (RTE). The RTE 
      cells were treated with 0, 100 mug/L and 10,000 mug/L of the AgNPs with diameters 
      of 10 nm and 100 nm for 12 hr. The cell inhibition level, apoptosis ratio, 
      reactive oxygen species (ROS), malondialdehyde (MDA) and metallothionein (MT) 
      content were determined. The mRNA expression of cytoc, caspase 3, and caspase 9 
      was measured by quantitative real-time polymerase chain reaction (qRT-PCR). In 
      addition, we also analyzed the cytoc, caspase 3, pro-caspase 3, caspase 9, and 
      pro-caspase 9 protein expression by western blotting. Electric cell-substrate 
      impedance sensing (ECIS) analysis showed that the growth and proliferation of RTE 
      cells were significantly inhibited in a dose-dependent manner under AgNPs 
      exposure. The cell dynamic changes induced by 10 nm AgNPs were more severe than 
      that of the 100 nm AgNPs exposure group. The intracellular MT, ROS, and MDA 
      content increased when the exposure concentration increased and size reduced, 
      whereas Ca(2+)-ATPase activity and Na(+)/K(+)-ATPase activity changed inversely. 
      The relative expression of protein of cytoc, caspase 3, and caspase 9 were 
      upregulated significantly, which indicated that AgNPs induced apoptosis of RTE 
      cells through the caspase-dependent mitochondrial pathway. Our results 
      demonstrate that AgNPs caused obvious cytotoxicity, oxidative stress, and 
      apoptosis in RTE cells, which promoted the releasing of cytochrome C and 
      pro-apoptotic proteins into the cytoplasm to activate the caspase cascade and 
      finally led to apoptosis.
FAU - Tang, Juan
AU  - Tang J
AD  - College of Life and Environmental Sciences, Hangzhou Normal University, China.
FAU - Lu, Xiangjun
AU  - Lu X
AD  - College of Life and Environmental Sciences, Hangzhou Normal University, China.
FAU - Chen, Bin
AU  - Chen B
AD  - College of Life and Environmental Sciences, Hangzhou Normal University, China.
FAU - Cai, Enqi
AU  - Cai E
AD  - College of Life and Environmental Sciences, Hangzhou Normal University, China.
FAU - Liu, Wenli
AU  - Liu W
AD  - College of Life and Environmental Sciences, Hangzhou Normal University, China.
FAU - Jiang, Jinxiao
AU  - Jiang J
AD  - College of Life and Environmental Sciences, Hangzhou Normal University, China.
FAU - Chen, Feifei
AU  - Chen F
AD  - College of Life and Environmental Sciences, Hangzhou Normal University, China.
FAU - Shan, Xiaodong
AU  - Shan X
AD  - College of Life and Environmental Sciences, Hangzhou Normal University, China.
FAU - Zhang, Hangjun
AU  - Zhang H
AD  - College of Life and Environmental Sciences, Hangzhou Normal University, China.
AD  - Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration, 
      Hangzhou Normal University, China.
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - J Toxicol Sci
JT  - The Journal of toxicological sciences
JID - 7805798
RN  - 0 (Cation Transport Proteins)
RN  - 0 (Reactive Oxygen Species)
RN  - 3M4G523W1G (Silver)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 3.4.22.- (Casp3 protein, rat)
RN  - EC 3.4.22.- (Casp9 protein, rat)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspase 9)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Caspase 3/genetics/metabolism
MH  - Caspase 9/genetics/metabolism
MH  - Cation Transport Proteins/metabolism
MH  - Cell Line
MH  - Epithelial Cells/*drug effects/metabolism
MH  - Malondialdehyde/metabolism
MH  - Metal Nanoparticles/*toxicity
MH  - Oxidative Stress/drug effects
MH  - Rats
MH  - Reactive Oxygen Species/metabolism
MH  - Silver/*toxicity
MH  - Trachea/cytology
OTO - NOTNLM
OT  - Apoptosis
OT  - Cytotoxicity
OT  - Oxidative stress
OT  - Rat tracheal epithelial cells
OT  - Silver nanoparticles
EDAT- 2019/03/08 06:00
MHDA- 2019/03/29 06:00
CRDT- 2019/03/08 06:00
PHST- 2019/03/08 06:00 [entrez]
PHST- 2019/03/08 06:00 [pubmed]
PHST- 2019/03/29 06:00 [medline]
AID - 10.2131/jts.44.155 [doi]
PST - ppublish
SO  - J Toxicol Sci. 2019;44(3):155-165. doi: 10.2131/jts.44.155.