PMID- 30843474
OWN - NLM
STAT- MEDLINE
DCOM- 20201013
LR  - 20201013
IS  - 1557-9042 (Electronic)
IS  - 0897-7151 (Linking)
VI  - 36
IP  - 14
DP  - 2019 Jul 15
TI  - Mild Closed-Head Injury in Conscious Rats Causes Transient Neurobehavioral and 
      Glial Disturbances: A Novel Experimental Model of Concussion.
PG  - 2260-2271
LID - 10.1089/neu.2018.6169 [doi]
AB  - Rodent models can provide insights into the most pertinent issues surrounding 
      concussion. Nonetheless, the relevance of some existing models to clinical 
      concussion can be questioned, particularly with regard to the use of surgery and 
      anesthesia and the mechanism and severity of injury. Accordingly, we have 
      co-developed an awake closed-head injury (ACHI) model in rats. Here, we aimed to 
      create a temporal profile of the neurobehavioral and neuropathological effects of 
      a single ACHI. Adolescent male rats were placed in a restraint bag and a steel 
      helmet was positioned over the head such that the impact target was centered over 
      the left parietal cortex. Once positioned on a foam platform, a cortical impactor 
      was used to strike the helmet. Sham animals underwent the same procedure without 
      impact. When compared with sham rats, those given a single ACHI displayed 
      evidence of sensorimotor deficits and reduced exploratory behavior within the 
      first 20 min post-injury; however, these effects were resolved after 24 h. A 
      single ACHI impaired spatial memory on the Y-maze task at both 5 min and 24 h 
      post-ACHI; however, no deficits were apparent at 48 h. Immunostaining revealed 
      region-specific increases in ionized calcium-binding adaptor molecule 1 and glial 
      fibrillary acidic protein expression at 3 days post-impact, with no differences 
      found at either 1 or 14 days. Taken together, our findings indicate that a single 
      ACHI results in transient neurobehavioral and glial disturbances and as such, 
      this model may be a valuable tool for pre-clinical concussion research.
FAU - Pham, Louise
AU  - Pham L
AD  - 1 Department of Physiology, Anatomy, and Microbiology, La Trobe University, 
      Bundoora, Australia.
FAU - Shultz, Sandy R
AU  - Shultz SR
AD  - 2 Department Neuroscience, Monash University, Melbourne, Australia.
AD  - 3 Department of Medicine, University of Melbourne, Melbourne, Australia.
FAU - Kim, Hyun Ah
AU  - Kim HA
AD  - 1 Department of Physiology, Anatomy, and Microbiology, La Trobe University, 
      Bundoora, Australia.
FAU - Brady, Rhys D
AU  - Brady RD
AD  - 2 Department Neuroscience, Monash University, Melbourne, Australia.
AD  - 3 Department of Medicine, University of Melbourne, Melbourne, Australia.
FAU - Wortman, Ryan C
AU  - Wortman RC
AD  - 2 Department Neuroscience, Monash University, Melbourne, Australia.
FAU - Genders, Shannyn G
AU  - Genders SG
AD  - 1 Department of Physiology, Anatomy, and Microbiology, La Trobe University, 
      Bundoora, Australia.
FAU - Hale, Matthew W
AU  - Hale MW
AD  - 4 Department of Psychology and Counseling, La Trobe University, Bundoora, 
      Australia.
FAU - O'Shea, Ross D
AU  - O'Shea RD
AD  - 1 Department of Physiology, Anatomy, and Microbiology, La Trobe University, 
      Bundoora, Australia.
FAU - Djouma, Elvan
AU  - Djouma E
AD  - 1 Department of Physiology, Anatomy, and Microbiology, La Trobe University, 
      Bundoora, Australia.
FAU - van den Buuse, Maarten
AU  - van den Buuse M
AD  - 4 Department of Psychology and Counseling, La Trobe University, Bundoora, 
      Australia.
AD  - 5 Department of Pharmacology, University of Melbourne, Melbourne, Australia.
AD  - 6 The College of Public Health, Medical, and Veterinary Sciences, James Cook 
      University, Queensland, Australia.
FAU - Church, Jarrod E
AU  - Church JE
AD  - 1 Department of Physiology, Anatomy, and Microbiology, La Trobe University, 
      Bundoora, Australia.
FAU - Christie, Brian R
AU  - Christie BR
AD  - 7 Division of Medical Sciences, University of Victoria, Victoria, British 
      Columbia, Canada.
FAU - Drummond, Grant R
AU  - Drummond GR
AD  - 1 Department of Physiology, Anatomy, and Microbiology, La Trobe University, 
      Bundoora, Australia.
FAU - Sobey, Christopher G
AU  - Sobey CG
AD  - 1 Department of Physiology, Anatomy, and Microbiology, La Trobe University, 
      Bundoora, Australia.
FAU - McDonald, Stuart J
AU  - McDonald SJ
AD  - 1 Department of Physiology, Anatomy, and Microbiology, La Trobe University, 
      Bundoora, Australia.
AD  - 2 Department Neuroscience, Monash University, Melbourne, Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190410
PL  - United States
TA  - J Neurotrauma
JT  - Journal of neurotrauma
JID - 8811626
SB  - IM
MH  - Animals
MH  - Brain Concussion/*physiopathology
MH  - Consciousness
MH  - *Disease Models, Animal
MH  - Exploratory Behavior
MH  - Head Injuries, Closed/physiopathology
MH  - Male
MH  - Maze Learning
MH  - *Neuroglia
MH  - Rats
MH  - Rats, Long-Evans
OTO - NOTNLM
OT  - anesthesia
OT  - astrocytes
OT  - behavior
OT  - microglia
OT  - mild traumatic brain injury
OT  - neuroinflammation
EDAT- 2019/03/08 06:00
MHDA- 2020/10/21 06:00
CRDT- 2019/03/08 06:00
PHST- 2019/03/08 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
PHST- 2019/03/08 06:00 [entrez]
AID - 10.1089/neu.2018.6169 [doi]
PST - ppublish
SO  - J Neurotrauma. 2019 Jul 15;36(14):2260-2271. doi: 10.1089/neu.2018.6169. Epub 
      2019 Apr 10.