PMID- 3084416
OWN - NLM
STAT- MEDLINE
DCOM- 19860625
LR  - 20131121
IS  - 0910-5050 (Print)
IS  - 0910-5050 (Linking)
VI  - 77
IP  - 3
DP  - 1986 Mar
TI  - Production of immunoreactive calcitonin and some other tumor markers by 
      established human carcinoma cell lines.
PG  - 270-5
AB  - Out of seven human carcinoma cell lines (M7609, CCK-81, FCC-1, RPMI#4788, QGP-1, 
      HLC-1, and KNS-62), 4 cell lines were found to produce immunoreactive calcitonin 
      (ICT), a potential tumor marker for various malignancies. During a 7-day culture, 
      1.4 X 10(5) QGP-1, RPMI#4788, HLC-1, and KNS-62 cells secreted 7,000 pg, 500 pg, 
      400 pg, and 400 pg of ICT in the medium, respectively. The production of ICT by 
      QGP-1 cells was increased by addition of pentagastrin or calcium gluconate. Three 
      different components of ICT (peak I, molecular weight greater than 40,000; peak 
      II, 14,000-18,000; peak III, 3,400) were detected by gel filtration of the QGP-1 
      spent medium. In a competitive inhibition-type radioimmunoassay of serial 
      dilutions of each ICT component, peak III component showed very similar 
      immunoreactivity to synthetic calcitonin. However, the other two components gave 
      clearly different immunoreactivities from the peak III component and showed very 
      similar immunoreactivities to each other. All the cell lines were further 
      screened for synthesis of 7 other tumor markers, carcinoembryonic antigen, 
      nonspecific cross-reacting antigen, CA19-9, tissue polypeptide antigen, 
      alpha-fetoprotein, beta 2-microglobulin and ferritin. Every cell line produced 2 
      to 6 markers concomitantly, and various combinations of positive markers were 
      found among the cell lines.
FAU - Ichiki, S
AU  - Ichiki S
FAU - Kuroki, M
AU  - Kuroki M
FAU - Matsunaga, A
AU  - Matsunaga A
FAU - Kuroki, M
AU  - Kuroki M
FAU - Matsuoka, Y
AU  - Matsuoka Y
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Jpn J Cancer Res
JT  - Japanese journal of cancer research : Gann
JID - 8509412
RN  - 0 (Culture Media)
RN  - 9007-12-9 (Calcitonin)
RN  - EF0NX91490 (Pentagastrin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcitonin/*biosynthesis/immunology
MH  - Calcium/pharmacology
MH  - Carcinoma/*metabolism
MH  - Cell Line
MH  - Colonic Neoplasms/metabolism
MH  - Culture Media
MH  - Humans
MH  - Lung Neoplasms/metabolism
MH  - Pancreatic Neoplasms/metabolism
MH  - Pentagastrin/pharmacology
MH  - Radioimmunoassay
EDAT- 1986/03/01 00:00
MHDA- 1986/03/01 00:01
CRDT- 1986/03/01 00:00
PHST- 1986/03/01 00:00 [pubmed]
PHST- 1986/03/01 00:01 [medline]
PHST- 1986/03/01 00:00 [entrez]
PST - ppublish
SO  - Jpn J Cancer Res. 1986 Mar;77(3):270-5.