PMID- 30844473
OWN - NLM
STAT- MEDLINE
DCOM- 20190528
LR  - 20190528
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 130
DP  - 2019 May
TI  - Protein extract from Cereus jamacaru (DC.) inhibits Colletotrichum 
      gloeosporioides growth by stimulating ROS generation and promoting severe cell 
      membrane damage.
PG  - 71-80
LID - S0882-4010(19)30024-5 [pii]
LID - 10.1016/j.micpath.2019.02.033 [doi]
AB  - Mandacaru (Cereus jamacaru DC.), is a cactaceous symbol of caatinga vegetation at 
      Brazilian Northeast region, however, there are no much studies about biochemical 
      properties of this species. Here, the pioneering study brings very relevant data 
      to highlight the importance of research with endemic plants of the caatinga. 
      Afterward, the presence of enzymes such as peroxidase, protease, chitinase, 
      beta-1,3-glucanase, and serine (trypsin) and cysteine (papain) protease inhibitors 
      were evaluated. The peroxidase activity was higher in roots than other tissues. 
      The beta-1,3-glucanase and proteolytic activity were prominent in stem and roots. 
      The chitinase activity and protease inhibitor for both classes analyzed were 
      detected in the stem and fruit peel. Antifungal activity against Colletotrichum 
      gloeosporioides showed the root extract has a promising inhibitory activity on 
      this economical important phytopathogenic fungus. After the contact of the hyphae 
      with root extract increase in membrane permeability, based on Propidium Iodide 
      (PI) uptake, and production of reactive oxygen species (ROS) were detected, 
      compared to negative control. In addition, Scanning Electron Microscopy (SEM) 
      analysis showed morphological damage on hyphae structure indicating that the 
      treatment debilitates either cell membrane or cell wall leading to the cell death 
      C. gloeosporioides.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Mota, Tarsila R
AU  - Mota TR
AD  - Department of Biological Science, State University of Rio Grande do Norte, RN, 
      Brazil.
FAU - Linhares, Havila V S
AU  - Linhares HVS
AD  - Department of Biological Science, State University of Rio Grande do Norte, RN, 
      Brazil.
FAU - Araujo-Filho, Jose H
AU  - Araujo-Filho JH
AD  - Department of Biological Science, State University of Rio Grande do Norte, RN, 
      Brazil.
FAU - Veras, Daniel M
AU  - Veras DM
AD  - Physical Therapy Department, Diocesan College, RN, Brazil.
FAU - Costa, Helen P S
AU  - Costa HPS
AD  - Department of Biochemistry and Molecular Biology, Federal University of Ceara, 
      CE, Brazil.
FAU - Souza, Carla M P
AU  - Souza CMP
AD  - Department of Animal Science, Federal Rural University of the Semi-Arid Region, 
      RN, Brazil.
FAU - Souza, Pedro F N
AU  - Souza PFN
AD  - Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, USA; 
      Department of Biochemistry and Molecular Biology, Federal University of Ceara, 
      CE, Brazil. Electronic address: pedrofilhobio@gmail.com.
FAU - Martins, Thiago F
AU  - Martins TF
AD  - Department of Biochemistry and Molecular Biology, Federal University of Ceara, 
      CE, Brazil. Electronic address: thiagofbioq@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20190305
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (Antifungal Agents)
RN  - 0 (Enzymes)
RN  - 0 (Plant Proteins)
RN  - 0 (Reactive Oxygen Species)
SB  - IM
MH  - Antifungal Agents/isolation & purification/*pharmacology
MH  - Cactaceae/*chemistry/enzymology
MH  - Cell Membrane/*drug effects/*pathology
MH  - Colletotrichum/drug effects/enzymology/*growth & development/ultrastructure
MH  - Enzymes/analysis
MH  - Fruit/chemistry/enzymology
MH  - Hyphae/ultrastructure
MH  - Microbial Viability/drug effects
MH  - Microscopy, Electron, Scanning
MH  - Permeability/drug effects
MH  - Plant Proteins/isolation & purification/*pharmacology
MH  - Plant Roots/chemistry/enzymology
MH  - Plant Stems/chemistry/enzymology
MH  - Reactive Oxygen Species/*metabolism
OTO - NOTNLM
OT  - Antifungal activity
OT  - Colletotrichum gloeosporioides
OT  - Enzymatic activity
OT  - Mandacaru
OT  - Phytopathogenic fungi
OT  - Protein extracts
EDAT- 2019/03/08 06:00
MHDA- 2019/05/29 06:00
CRDT- 2019/03/08 06:00
PHST- 2019/01/05 00:00 [received]
PHST- 2019/02/25 00:00 [revised]
PHST- 2019/02/28 00:00 [accepted]
PHST- 2019/03/08 06:00 [pubmed]
PHST- 2019/05/29 06:00 [medline]
PHST- 2019/03/08 06:00 [entrez]
AID - S0882-4010(19)30024-5 [pii]
AID - 10.1016/j.micpath.2019.02.033 [doi]
PST - ppublish
SO  - Microb Pathog. 2019 May;130:71-80. doi: 10.1016/j.micpath.2019.02.033. Epub 2019 
      Mar 5.