PMID- 30846312
OWN - NLM
STAT- MEDLINE
DCOM- 20190705
LR  - 20190705
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1595
DP  - 2019 Jun 21
TI  - Integrated strategy for the extraction and profiling of bioactive metabolites 
      from Passiflora mollissima seeds combining pressurized-liquid extraction and 
      gas/liquid chromatography-high resolution mass spectrometry.
PG  - 144-157
LID - S0021-9673(19)30162-1 [pii]
LID - 10.1016/j.chroma.2019.02.031 [doi]
AB  - An integrated analytical methodology based on pressurized-liquid extraction (PLE) 
      in two steps, followed by in vitro assays and liquid chromatography/gas 
      chromatography coupled to high-resolution mass spectrometry (HRMS), was developed 
      and applied for the isolation and characterization of potential bioactive 
      metabolites from Passiflora mollissima seeds. PLE was proposed in two sequential 
      steps: 1) recovery of the lipidic fraction using nonpolar solvents, and 2) 
      recovery of the phenolic fraction from the defatted seeds' residue using polar 
      solvents. Cyclohexane was selected as the most suitable extraction solvent for 
      the seeds defatting process (20 min, 100  degrees C and 100 bar). PLE optimization by 
      response surface methodology was carried out to obtain phenolics-rich extracts 
      with the highest antioxidant activity. Optimal extraction yield (6.58%), total 
      phenolic content (29.99 mg g(-1)), total flavonoids content (0.94 mg g(-1)) and 
      antioxidant activity (6.94 mM trolox g(-1) and EC(50) of 2.66 mug mL(-1)) were 
      obtained operating at 150  degrees C with EtOH (100%) as solvent. Untargeted and 
      semi-targeted MS and MS/MS data-mining strategies were successfully implemented 
      for the rapid and comprehensive profiling of the polar and lipidic PLE fractions 
      analysed by UHPLC and GC, respectively, coupled to quadrupole time-of-flight mass 
      spectrometry (q-TOF-MS/MS). Polyphenols-rich extracts from P. mollisima seeds 
      were characterized for the first time applying this approach, showing a broad 
      variety of flavonoids, genuine flavanols (e.g. (epi)fisetinidol) and abundant 
      proanthocyanidins. This application can be considered a successful demonstration 
      of the great potential of the proposed methodology to effectively obtain and 
      characterize complex natural extracts with potential bioactivity, by making use 
      of powerful integrated identification strategies to facilitate the challenging 
      post-acquisition data processing of huge datasets generated by HRMS analysis.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Ballesteros-Vivas, Diego
AU  - Ballesteros-Vivas D
AD  - High Pressure Laboratory, Department of Chemistry, Faculty of Science, 
      Universidad Nacional de Colombia, Carrera 30 #45-03, Bogota D.C., 111321, 
      Colombia; Laboratory of Foodomics, Institute of Food Science Research, CIAL, 
      CSIC, Nicolas Cabrera 9, 28049 Madrid, Spain.
FAU - Alvarez-Rivera, Gerardo
AU  - Alvarez-Rivera G
AD  - Laboratory of Foodomics, Institute of Food Science Research, CIAL, CSIC, Nicolas 
      Cabrera 9, 28049 Madrid, Spain.
FAU - Ibanez, Elena
AU  - Ibanez E
AD  - Laboratory of Foodomics, Institute of Food Science Research, CIAL, CSIC, Nicolas 
      Cabrera 9, 28049 Madrid, Spain.
FAU - Parada-Alfonso, Fabian
AU  - Parada-Alfonso F
AD  - High Pressure Laboratory, Department of Chemistry, Faculty of Science, 
      Universidad Nacional de Colombia, Carrera 30 #45-03, Bogota D.C., 111321, 
      Colombia.
FAU - Cifuentes, Alejandro
AU  - Cifuentes A
AD  - Laboratory of Foodomics, Institute of Food Science Research, CIAL, CSIC, Nicolas 
      Cabrera 9, 28049 Madrid, Spain. Electronic address: a.cifuentes@csic.es.
LA  - eng
PT  - Journal Article
DEP - 20190214
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Antioxidants)
RN  - 0 (Flavonoids)
RN  - 0 (Phenols)
RN  - 0 (Plant Extracts)
RN  - 0 (Polyphenols)
RN  - 0 (Solvents)
SB  - IM
MH  - Antioxidants/chemistry/isolation & purification
MH  - Chemistry Techniques, Analytical/*methods
MH  - Flavonoids/analysis
MH  - *Gas Chromatography-Mass Spectrometry
MH  - Liquid-Liquid Extraction
MH  - Passiflora/*chemistry
MH  - Phenols/analysis
MH  - Plant Extracts/*chemistry/*isolation & purification
MH  - Polyphenols/*chemistry/*isolation & purification
MH  - Seeds/*chemistry
MH  - Solvents/analysis
OTO - NOTNLM
OT  - Fruit by-products
OT  - GC-q-TOF-MS
OT  - High-resolution mass spectrometry
OT  - LC-q-TOF-MS
OT  - MS data-mining strategies
OT  - Metabolites profiling
OT  - PLE
OT  - Passiflora mollissima
OT  - Pressurized-liquid extraction
EDAT- 2019/03/09 06:00
MHDA- 2019/07/06 06:00
CRDT- 2019/03/09 06:00
PHST- 2018/12/20 00:00 [received]
PHST- 2019/02/04 00:00 [revised]
PHST- 2019/02/13 00:00 [accepted]
PHST- 2019/03/09 06:00 [pubmed]
PHST- 2019/07/06 06:00 [medline]
PHST- 2019/03/09 06:00 [entrez]
AID - S0021-9673(19)30162-1 [pii]
AID - 10.1016/j.chroma.2019.02.031 [doi]
PST - ppublish
SO  - J Chromatogr A. 2019 Jun 21;1595:144-157. doi: 10.1016/j.chroma.2019.02.031. Epub 
      2019 Feb 14.