PMID- 30849500 OWN - NLM STAT- MEDLINE DCOM- 20190903 LR - 20190903 IS - 1095-9947 (Electronic) IS - 1050-4648 (Linking) VI - 88 DP - 2019 May TI - Molecular cloning, characterization and expression analysis of S- adenosyl- L-homocysteine hydrolase (SAHH) during the pathogenic infection of Litopenaeus vannamei by Vibrio alginolyticus. PG - 284-292 LID - S1050-4648(19)30132-9 [pii] LID - 10.1016/j.fsi.2019.02.058 [doi] AB - SAHH is an enzyme, playing a significant role in the catalyzation of the S-adenosyl homocysteine (SAH) into homocysteine (Hcy) and adenosine (Ado). However, little is known information of the enzyme in crustaceans. In the present study, SAHH cDNA was cloned from Litopenaeus vannamei (LvSAHH). The full length of the LvSAHH was found, containing a 5' UTR of 119 bp, an ORF of 1236 bp and a 3' UTR of 549 bp. The LvSAHH gene encoded a polypeptide of 411 amino acids with an estimated molecular mass of 45.55 kD and a predicted isoelectronic point (pI) of 5.63. Comparison of the deduced amino acid sequence showed that LvSAHH has high identity (70 %-82%) with other known species. qRT-PCR analysis revealed that LvSAHH mRNA was broadly expressed in all of the examined tissues, while the highest expression level was observed in muscle, followed by the expression in stomach, gill, pleopod, hepatopancreas, heart, eye and intestine. Subcellular localization analysis revealed that LvSAHH was predominantly localized in the cytoplasm and nucleus. LvSAHH mRNA expression levels in hepatopancreas and gill were significantly up-regulated from 6 to 48 h after V. alginolyticus injection and reached the highest level (15-fold and 8-fold, p < 0.01) at 24 h, respectively. Additionally, the Toll-like receptors (TLR) and interleukins-16 (IL-16) were detected in hepatopancreas and gill of LvSAHH-knockdown SAHH. LvRack1, LvToll1, LvToll2, LvToll3 and LvIL-16 transcripts were decreased significantly in LvSAHH-knockdown shrimp at 24 h post V. alginolyticus stimulation in hepatopancreas and gill. But LvToll3 was no significant difference in gill. In summary, these results indicated that LvSAHH may play a regulatory role in the invertebrate innate immune defense by regulating TLR and IL-16 expression. CI - Copyright (c) 2019. Published by Elsevier Ltd. FAU - Liang, QingJian AU - Liang Q AD - College of Life Science, South China Normal University, Guangzhou, 510631, PR China; Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, PR China; Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, PR China. FAU - Ou, MuFei AU - Ou M AD - College of Life Science, South China Normal University, Guangzhou, 510631, PR China; Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, PR China; Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, PR China. FAU - Ren, YingHao AU - Ren Y AD - College of Life Science, South China Normal University, Guangzhou, 510631, PR China; Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, PR China; Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, PR China. FAU - Yao, ZeNa AU - Yao Z AD - College of Life Science, South China Normal University, Guangzhou, 510631, PR China; Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, PR China; Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, PR China. FAU - Hu, Rui AU - Hu R AD - College of Life Science, South China Normal University, Guangzhou, 510631, PR China; Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, PR China; Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, PR China. FAU - Li, JieZhen AU - Li J AD - College of Life Science, South China Normal University, Guangzhou, 510631, PR China; Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, PR China; Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, PR China. FAU - Liu, Yuan AU - Liu Y AD - College of Life Science, South China Normal University, Guangzhou, 510631, PR China; Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, PR China; Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, PR China. FAU - Wang, Weina AU - Wang W AD - College of Life Science, South China Normal University, Guangzhou, 510631, PR China; Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, PR China; Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, PR China. Electronic address: wangwn@scnu.edu.cn. LA - eng PT - Journal Article DEP - 20190305 PL - England TA - Fish Shellfish Immunol JT - Fish & shellfish immunology JID - 9505220 RN - 0 (Interleukin-16) RN - 0 (RNA, Messenger) RN - 0 (Toll-Like Receptors) RN - EC 3.3.1.1 (Adenosylhomocysteinase) SB - IM MH - Adenosylhomocysteinase/genetics/*metabolism MH - Amino Acid Sequence MH - Animals MH - Cloning, Molecular MH - Gene Expression MH - Gene Knockdown Techniques MH - Immunity, Innate/genetics MH - Interleukin-16/metabolism MH - Penaeidae/enzymology/*immunology/microbiology MH - RNA, Messenger/metabolism MH - Toll-Like Receptors/metabolism MH - *Vibrio alginolyticus OTO - NOTNLM OT - Litopenaeus vannamei OT - Rack1 OT - SAHH OT - TLR EDAT- 2019/03/09 06:00 MHDA- 2019/09/04 06:00 CRDT- 2019/03/09 06:00 PHST- 2018/11/26 00:00 [received] PHST- 2019/02/22 00:00 [revised] PHST- 2019/02/25 00:00 [accepted] PHST- 2019/03/09 06:00 [pubmed] PHST- 2019/09/04 06:00 [medline] PHST- 2019/03/09 06:00 [entrez] AID - S1050-4648(19)30132-9 [pii] AID - 10.1016/j.fsi.2019.02.058 [doi] PST - ppublish SO - Fish Shellfish Immunol. 2019 May;88:284-292. doi: 10.1016/j.fsi.2019.02.058. Epub 2019 Mar 5.