PMID- 30850859
OWN - NLM
STAT- MEDLINE
DCOM- 20200106
LR  - 20200305
IS  - 1436-3771 (Electronic)
IS  - 1432-6981 (Linking)
VI  - 23
IP  - 11
DP  - 2019 Nov
TI  - The interactions of dendritic cells with osteoblasts on titanium surfaces: an in 
      vitro investigation.
PG  - 4133-4143
LID - 10.1007/s00784-019-02852-w [doi]
AB  - OBJECTIVES: Osteoimmune interactions possess a critical part in the integration 
      of materials and hosts. Dendritic cells (DCs) are the most common members of 
      osteoimmune cells family. The titanium surfaces of dental implants tend to 
      promote a mature dendritic cell phenotype with increased proinflammatory 
      secretion. However, very little is known to the effects of this microenvironment 
      on the behaviors of cells around implants, especially osteoblasts, and how the 
      tissue integrations take place on such biomaterial surfaces. Therefore, the 
      present study was to investigate the interactions of DCs with osteoblasts on 
      titanium surfaces. DCs seeded on PT and SLA titanium surfaces were compared by 
      assays for the proliferations, surface markers, and inflammatory genes 
      expressions. MATERIALS AND METHODS: DCs seeded on PT and SLA titanium surfaces 
      were compared by assays for the proliferations, surface markers, and inflammatory 
      genes expressions. Next, we harvested the dendritic cell-conditioned medium (CM) 
      and investigated the effects of CM on MC3T3-E1. RESULTS: The results showed an 
      increase in CD86 and proinflammatory expressions of DCs seeded on PT and SLA 
      surfaces, as well as a decrease in anti-inflammatory cytokines. The CM from 
      titanium surfaces inhibited the osteoblast differentiation with reduced 
      expression of osteogenic genes RUNX2, COL1, ALP, and OCN and decreased ALP 
      activity as well as Alizarin red staining. CONCLUSION: These findings suggested 
      that titanium surfaces switch DCs toward maturation phenotypes and thus inhibit 
      the differentiation and mineralization of osteoblasts. CLINICAL RELEVANCE: 
      Knowing the significant impact of immune cells on osteogenesis behaviors, some 
      efforts to decrease the immune reaction might be of clinical significance. 
      Favorable immune environments can increase the dental implants survival rate in 
      patients.
FAU - Yang, Yang
AU  - Yang Y
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School 
      and Hospital of Stomatology, Wuhan University, Wuhan, China.
AD  - Department of Oral Implantology, Guangdong Provincial Key Laboratory of 
      Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, 510055, 
      Guangzhou, People's Republic of China.
FAU - Wang, Xuzhu
AU  - Wang X
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School 
      and Hospital of Stomatology, Wuhan University, Wuhan, China.
FAU - Miron, Richard J
AU  - Miron RJ
AD  - Department of Periodontology, College of Dental Medicine, Nova Southeastern 
      University, Fort Lauderdale, FL, 33328, USA.
FAU - Zhang, Xiaoxin
AU  - Zhang X
AUID- ORCID: 0000-0002-6899-924X
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School 
      and Hospital of Stomatology, Wuhan University, Wuhan, China. 
      zhangxiaoxin@whu.edu.cn.
LA  - eng
GR  - 81600906/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20190309
PL  - Germany
TA  - Clin Oral Investig
JT  - Clinical oral investigations
JID - 9707115
RN  - D1JT611TNE (Titanium)
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - *Dendritic Cells
MH  - Humans
MH  - *Osteoblasts
MH  - *Osteogenesis
MH  - Surface Properties
MH  - *Titanium
OTO - NOTNLM
OT  - Dendritic cell
OT  - Immune response
OT  - Osteogenesis
OT  - Titanium surface
EDAT- 2019/03/10 06:00
MHDA- 2020/01/07 06:00
CRDT- 2019/03/10 06:00
PHST- 2018/09/18 00:00 [received]
PHST- 2019/02/13 00:00 [accepted]
PHST- 2019/03/10 06:00 [pubmed]
PHST- 2020/01/07 06:00 [medline]
PHST- 2019/03/10 06:00 [entrez]
AID - 10.1007/s00784-019-02852-w [pii]
AID - 10.1007/s00784-019-02852-w [doi]
PST - ppublish
SO  - Clin Oral Investig. 2019 Nov;23(11):4133-4143. doi: 10.1007/s00784-019-02852-w. 
      Epub 2019 Mar 9.