PMID- 30852268
OWN - NLM
STAT- MEDLINE
DCOM- 20191022
LR  - 20191210
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 531
DP  - 2019 May
TI  - A high-throughput screen for genes essential for PRRSV infection using a 
      piggyBac-based system.
PG  - 19-30
LID - S0042-6822(19)30066-2 [pii]
LID - 10.1016/j.virol.2019.03.001 [doi]
AB  - In this study, using a dual-functional, piggyBac transposon-based system, we 
      developed a method to systematically decipher the host genes that may be 
      associated with porcine reproductive and respiratory syndrome virus (PRRSV) 
      infection. A Marc145 cell library, which was randomly mutated by transfecting 
      piggyBac plasmids, was challenged with PRRSV. The surviving cell clones were 
      subjected to inverse PCR and high-throughput sequencing to map the integration 
      sites of the transposon. Detailed annotation of the genes flanking the 
      integration sites allowed us to generate a ranked list of candidate genes. Among 
      the predicted genes with a high priority, four genes, CDK17, RNF168, BCL2L15, and 
      TRIM33, were strongly correlated with PRRSV infection in both Marc145 cells and 
      porcine primary alveolar macrophages. This study not only assists in identifying 
      the genes essential for PRRSV infection but also confirms the possibility of 
      using the piggyBac system to study other virus-host genetic interactions in a 
      high-throughput manner.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Bai, Jianhui
AU  - Bai J
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, 
      National Engineering Laboratory for Animal Breeding, China Agricultural 
      University, Beijing 100193, PR China.
FAU - Li, Kongpan
AU  - Li K
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, 
      National Engineering Laboratory for Animal Breeding, China Agricultural 
      University, Beijing 100193, PR China.
FAU - Tang, Wenda
AU  - Tang W
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, 
      National Engineering Laboratory for Animal Breeding, China Agricultural 
      University, Beijing 100193, PR China.
FAU - Liang, Zuoxiang
AU  - Liang Z
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, 
      National Engineering Laboratory for Animal Breeding, China Agricultural 
      University, Beijing 100193, PR China.
FAU - Wang, Xifeng
AU  - Wang X
AD  - Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, 
      Chinese Academy of Science, Beijing 100101, PR China.
FAU - Feng, Wenhai
AU  - Feng W
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, 
      National Engineering Laboratory for Animal Breeding, China Agricultural 
      University, Beijing 100193, PR China.
FAU - Zhang, Shujun
AU  - Zhang S
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of 
      Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, PR China.
FAU - Ren, Liming
AU  - Ren L
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, 
      National Engineering Laboratory for Animal Breeding, China Agricultural 
      University, Beijing 100193, PR China.
FAU - Wu, Sen
AU  - Wu S
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, 
      National Engineering Laboratory for Animal Breeding, China Agricultural 
      University, Beijing 100193, PR China. Electronic address: swu@cau.edu.cn.
FAU - Han, Haitang
AU  - Han H
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, 
      National Engineering Laboratory for Animal Breeding, China Agricultural 
      University, Beijing 100193, PR China. Electronic address: hanhaitang@cau.edu.cn.
FAU - Zhao, Yaofeng
AU  - Zhao Y
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, 
      National Engineering Laboratory for Animal Breeding, China Agricultural 
      University, Beijing 100193, PR China. Electronic address: yaofengzhao@cau.edu.cn.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190304
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (DNA Transposable Elements)
RN  - 0 (Transcription Factors)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinases)
SB  - IM
MH  - Animals
MH  - Cyclin-Dependent Kinases/genetics/metabolism
MH  - DNA Transposable Elements
MH  - Gene Expression Profiling
MH  - High-Throughput Screening Assays/*methods
MH  - Host-Pathogen Interactions
MH  - Macrophages, Alveolar/metabolism/virology
MH  - Plasmids/genetics/metabolism
MH  - Porcine Reproductive and Respiratory Syndrome/*genetics/metabolism/*virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*physiology
MH  - Swine
MH  - Transcription Factors/genetics/metabolism
MH  - Ubiquitin-Protein Ligases/genetics/metabolism
MH  - Virus Replication
OTO - NOTNLM
OT  - BCL2L15
OT  - CDK17
OT  - High-throughput screen
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - RNF168
OT  - TRIM33
OT  - piggyBac transposon
EDAT- 2019/03/11 06:00
MHDA- 2019/10/23 06:00
CRDT- 2019/03/11 06:00
PHST- 2018/12/05 00:00 [received]
PHST- 2019/03/02 00:00 [revised]
PHST- 2019/03/02 00:00 [accepted]
PHST- 2019/03/11 06:00 [pubmed]
PHST- 2019/10/23 06:00 [medline]
PHST- 2019/03/11 06:00 [entrez]
AID - S0042-6822(19)30066-2 [pii]
AID - 10.1016/j.virol.2019.03.001 [doi]
PST - ppublish
SO  - Virology. 2019 May;531:19-30. doi: 10.1016/j.virol.2019.03.001. Epub 2019 Mar 4.