PMID- 30852707
OWN - NLM
STAT- MEDLINE
DCOM- 20200330
LR  - 20200330
IS  - 1573-6849 (Electronic)
IS  - 0967-3849 (Linking)
VI  - 27
IP  - 3
DP  - 2019 Sep
TI  - Fluorescence in situ hybridization in plants: recent developments and future 
      applications.
PG  - 153-165
LID - 10.1007/s10577-019-09607-z [doi]
AB  - Fluorescence in situ hybridization (FISH) was developed more than 30 years ago 
      and has been the most paradigm-changing technique in cytogenetic research. FISH 
      has been used to answer questions related to structure, mutation, and evolution 
      of not only individual chromosomes but also entire genomes. FISH has served as an 
      important tool for chromosome identification in many plant species. This review 
      intends to summarize and discuss key technical development and applications of 
      FISH in plants since 2006. The most significant recent advance of FISH is the 
      development and application of probes based on synthetic oligonucleotides 
      (oligos). Oligos specific to a repetitive DNA sequence, to a specific chromosomal 
      region, or to an entire chromosome can be computationally identified, synthesized 
      in parallel, and fluorescently labeled. Oligo probes designed from conserved DNA 
      sequences from one species can be used among genetically related species, 
      allowing comparative cytogenetic mapping of these species. The advances with 
      synthetic oligo probes will significantly expand the applications of FISH 
      especially in non-model plant species. Recent achievements and future 
      applications of FISH and oligo-FISH are discussed.
FAU - Jiang, Jiming
AU  - Jiang J
AUID- ORCID: 0000-0002-6435-6140
AD  - Department of Plant Biology, Department of Horticulture, Michigan State 
      University, East Lansing, MI, 48824, USA. jiangjm@msu.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Review
DEP - 20190309
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - Chromosomes, Plant/*genetics
MH  - In Situ Hybridization, Fluorescence/methods/*trends
MH  - *Oligonucleotide Probes/chemical synthesis
OTO - NOTNLM
OT  - Chromosome
OT  - Evolution
OT  - FISH
OT  - Genome
OT  - Karyotype
OT  - Oligo-FISH
EDAT- 2019/03/11 06:00
MHDA- 2020/03/31 06:00
CRDT- 2019/03/11 06:00
PHST- 2019/01/11 00:00 [received]
PHST- 2019/03/01 00:00 [accepted]
PHST- 2019/02/27 00:00 [revised]
PHST- 2019/03/11 06:00 [pubmed]
PHST- 2020/03/31 06:00 [medline]
PHST- 2019/03/11 06:00 [entrez]
AID - 10.1007/s10577-019-09607-z [pii]
AID - 10.1007/s10577-019-09607-z [doi]
PST - ppublish
SO  - Chromosome Res. 2019 Sep;27(3):153-165. doi: 10.1007/s10577-019-09607-z. Epub 
      2019 Mar 9.