PMID- 30854065
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211215
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 17
IP  - 3
DP  - 2019 Mar
TI  - Claudin-7 modulates cell-matrix adhesion that controls cell migration, invasion 
      and attachment of human HCC827 lung cancer cells.
PG  - 2890-2896
LID - 10.3892/ol.2019.9909 [doi]
AB  - Claudins are a family of tight junction proteins, and serve important roles in 
      epithelial barrier, selective ion transports and cancer metastasis. Although the 
      exact role of claudin-7 in human lung cancer has not been completely elucidated, 
      recent clinical studies have demonstrated that claudin-7 is associated with the 
      survival of patients with lung cancer. Our previous studies have demonstrated 
      that claudin-7 forms a protein complex with integrin beta1 in human lung cancer 
      cells. The knockdown (KD) of claudin-7 by short hairpin RNA (shRNA) reduced 
      integrin beta1 expression and increased the cell proliferative rate, whereas 
      claudin-7 re-expression in the KD cells decreased the cell proliferation. It is 
      unknown as to whether claudin-7 and integrin beta1 regulate cell proliferation and 
      invasion synergistically or independently. In the present study, it was observed 
      that ectopic expression of integrin beta1 in claudin-7 KD lung cancer cells did not 
      reduce the cell proliferation. However, integrin beta1-transfected cells migrated 
      more effectively in wound healing and cell invasion assays and were more adhesive 
      in a cell attachment assay when compared with those of claudin-7 KD cells. This 
      indicates that claudin-7 controls cell proliferation, while cell attachment and 
      motility were regulated partially through integrin beta1. Additionally, claudin-7 
      overexpression in claudin-7 KD cells resulted in an improved ability to attach to 
      the surface of cell culture plates and a higher expression of focal adhesion 
      proteins when compared with claudin-7 non-KD control cells, which supports the 
      role of claudin-7 in cell adhesion and motility. Taken together, these data 
      suggest that claudin-7 regulates cell motility through integrin beta1, providing 
      additional insight into the roles of claudins in carcinogenesis and cancer cell 
      metastasis.
FAU - Kim, Do Hyung
AU  - Kim DH
AD  - Department of Anatomy and Cell Biology, Brody School of Medicine, East Carolina 
      University, Greenville, NC 27834, USA.
FAU - Lu, Qun
AU  - Lu Q
AD  - Department of Anatomy and Cell Biology, Brody School of Medicine, East Carolina 
      University, Greenville, NC 27834, USA.
AD  - Leo Jenkins Cancer Center, Brody School of Medicine, East Carolina University, 
      Greenville, NC 27834, USA.
FAU - Chen, Yan-Hua
AU  - Chen YH
AD  - Department of Anatomy and Cell Biology, Brody School of Medicine, East Carolina 
      University, Greenville, NC 27834, USA.
AD  - Leo Jenkins Cancer Center, Brody School of Medicine, East Carolina University, 
      Greenville, NC 27834, USA.
LA  - eng
GR  - R15 DK103166/DK/NIDDK NIH HHS/United States
PT  - Journal Article
DEP - 20190108
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC6365970
OTO - NOTNLM
OT  - cell attachment
OT  - cell invasion
OT  - cell migration
OT  - claudin-7
OT  - human lung cancer cells
OT  - tight junctions
EDAT- 2019/03/12 06:00
MHDA- 2019/03/12 06:01
PMCR- 2019/01/08
CRDT- 2019/03/12 06:00
PHST- 2018/08/07 00:00 [received]
PHST- 2018/12/31 00:00 [accepted]
PHST- 2019/03/12 06:00 [entrez]
PHST- 2019/03/12 06:00 [pubmed]
PHST- 2019/03/12 06:01 [medline]
PHST- 2019/01/08 00:00 [pmc-release]
AID - OL-0-0-9909 [pii]
AID - 10.3892/ol.2019.9909 [doi]
PST - ppublish
SO  - Oncol Lett. 2019 Mar;17(3):2890-2896. doi: 10.3892/ol.2019.9909. Epub 2019 Jan 8.