PMID- 30854734
OWN - NLM
STAT- MEDLINE
DCOM- 20200814
LR  - 20200814
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 120
IP  - 8
DP  - 2019 Aug
TI  - miR-193b-5p regulates chondrocytes metabolism by directly targeting histone 
      deacetylase 7 in interleukin-1beta-induced osteoarthritis.
PG  - 12775-12784
LID - 10.1002/jcb.28545 [doi]
AB  - There is increasing evidence regarding the pivotal roles of microRNAs (miRNAs) 
      and histone deacetylases (HDACs) in the development of osteoarthritis (OA). This 
      study aimed to determine whether miR-193b-5p regulates HDAC7 expression directly 
      to affect cartilage degeneration. Expression levels of miR-193b-5p, HDAC7, matrix 
      metalloproteinase 3 (MMP3), and MMP13 were determined in normal and OA cartilage 
      and primary human chondrocytes (PHCs) stimulated with interleukin-1beta (IL-1beta). 
      PHCs were transfected with a miR-193b-5p mimic or inhibitor to verify whether 
      miR-193b-5p influences the expression of HDAC7 and MMPs. A luciferase reporter 
      assay was performed to demonstrate the binding between miR-193b-5p and the 
      3'-untranslated region (UTR) of HDAC7. Expression of miR-193b-5p was reduced in 
      IL-1beta-stimulated PHCs and in OA cartilage compared to that in normal cartilage. 
      Luciferase reporter assay exhibited the repressed activity of the reporter 
      construct containing the 3'UTR of HDAC7. Both miR-193b-5p overexpression and 
      HDAC7 inhibition decreased the expression of MMP3 and MMP13, whereas the 
      inhibition of miR-193b-5p enhanced HDAC7, MMP3, and MMP13 expression. miR-193b-5p 
      downregulates HDAC7 directly and, as a result, inhibits MMP3 and MMP13 
      expression, which suggests that miR-193b-5p has a protective role in OA.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Zhang, Chengyun
AU  - Zhang C
AD  - Department of Joint Surgery, First Affiliated Hospital of Sun Yat-sen University, 
      Guangzhou, Guangdong, China.
FAU - Zhang, Zhiqi
AU  - Zhang Z
AD  - Department of Joint Surgery, First Affiliated Hospital of Sun Yat-sen University, 
      Guangzhou, Guangdong, China.
FAU - Chang, Zongkun
AU  - Chang Z
AUID- ORCID: 0000-0003-3970-3144
AD  - Department of Orthopaedics, The Seventh Affiliated Hospital, Sun Yat-sen 
      University, Shenzhen, Guangdong, China.
FAU - Mao, Guping
AU  - Mao G
AD  - Department of Joint Surgery, First Affiliated Hospital of Sun Yat-sen University, 
      Guangzhou, Guangdong, China.
FAU - Hu, Shu
AU  - Hu S
AD  - Department of Joint Surgery, First Affiliated Hospital of Sun Yat-sen University, 
      Guangzhou, Guangdong, China.
FAU - Zeng, Anyu
AU  - Zeng A
AD  - Department of Joint Surgery, First Affiliated Hospital of Sun Yat-sen University, 
      Guangzhou, Guangdong, China.
FAU - Fu, Ming
AU  - Fu M
AUID- ORCID: 0000-0002-5327-780X
AD  - Department of Joint Surgery, First Affiliated Hospital of Sun Yat-sen University, 
      Guangzhou, Guangdong, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190310
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Interleukin-1beta)
RN  - 0 (MIRN193 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 3.4.24.- (MMP13 protein, human)
RN  - EC 3.4.24.- (Matrix Metalloproteinase 13)
RN  - EC 3.4.24.17 (MMP3 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.5.1.98 (HDAC7 protein, human)
RN  - EC 3.5.1.98 (Histone Deacetylases)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Cells, Cultured
MH  - Chondrocytes/*cytology/drug effects/metabolism
MH  - Female
MH  - Histone Deacetylases/*genetics
MH  - Humans
MH  - Interleukin-1beta/*adverse effects
MH  - Male
MH  - Matrix Metalloproteinase 13/metabolism
MH  - Matrix Metalloproteinase 3/metabolism
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Osteoarthritis/*genetics/metabolism
MH  - Primary Cell Culture
OTO - NOTNLM
OT  - histone deacetylase 7
OT  - matrix metalloproteinase 13
OT  - matrix metalloproteinase 3
OT  - miR-193b-5p
OT  - microRNAs
OT  - osteoarthritis
EDAT- 2019/03/12 06:00
MHDA- 2020/08/15 06:00
CRDT- 2019/03/12 06:00
PHST- 2018/11/09 00:00 [received]
PHST- 2018/12/14 00:00 [revised]
PHST- 2019/01/10 00:00 [accepted]
PHST- 2019/03/12 06:00 [pubmed]
PHST- 2020/08/15 06:00 [medline]
PHST- 2019/03/12 06:00 [entrez]
AID - 10.1002/jcb.28545 [doi]
PST - ppublish
SO  - J Cell Biochem. 2019 Aug;120(8):12775-12784. doi: 10.1002/jcb.28545. Epub 2019 
      Mar 10.