PMID- 30854963
OWN - NLM
STAT- MEDLINE
DCOM- 20200609
LR  - 20200609
IS  - 1875-5666 (Electronic)
IS  - 1566-5240 (Linking)
VI  - 19
IP  - 2
DP  - 2019
TI  - Negative Correlation Between Serum Levels of Homocysteine and Apolipoprotein M.
PG  - 120-126
LID - 10.2174/1566524019666190308115624 [doi]
AB  - BACKGROUND: Homocysteine (Hcy) has been suggested as an independent risk factor 
      for atherosclerosis. Apolipoprotein M (apoM) is a constituent of the HDL 
      particles. The goal of this study was to examine the serum levels of homocysteine 
      and apoM and to determine whether homocysteine influences apoM synthesis. 
      METHODS: Serum levels of apoM and Hcy in 17 hyperhomocysteinemia (HHcy) patients 
      and 19 controls were measured and their correlations were analyzed. Different 
      concentrations of homocysteine (Hcy) and LY294002, a specific phosphoinositide 3- 
      kinase (PI3K) inhibitor, were used to treat HepG2 cells. The mRNA levels were 
      determined by RT-PCR and the apoM protein mass was measured by western blot. 
      RESULTS: We found that decreased serum apoM levels corresponded with serum HDL 
      levels in HHcy patients, while the serum apoM levels showed a statistically 
      significant negative correlation with the serum Hcy levels. Moreover, apoM mRNA 
      and protein levels were significantly decreased after the administration of Hcy 
      in HepG2 cells, and this effect could be abolished by addition of LY294002. 
      CONCLUSIONS: Present study demonstrates that Hcy downregulates the expression of 
      apoM by mechanisms involving the PI3K signal pathway.
CI  - Copyright(c) Bentham Science Publishers; For any queries, please email at 
      epub@benthamscience.net.
FAU - Wei, J
AU  - Wei J
AD  - Department of Comprehensive Laboratory, the Third Affiliated Hospital of Soochow 
      University, Changzhou 213003, China.
FAU - Yu, Y
AU  - Yu Y
AD  - Department of Comprehensive Laboratory, the Third Affiliated Hospital of Soochow 
      University, Changzhou 213003, China.
FAU - Feng, Y
AU  - Feng Y
AD  - Department of Comprehensive Laboratory, the Third Affiliated Hospital of Soochow 
      University, Changzhou 213003, China.
FAU - Zhang, J
AU  - Zhang J
AD  - Department of Comprehensive Laboratory, the Third Affiliated Hospital of Soochow 
      University, Changzhou 213003, China.
FAU - Jiang, Q
AU  - Jiang Q
AD  - Department of Laboratory Medicine, the Third Affiliated Hospital of Soochow 
      University, Changzhou 213003, China.
FAU - Zheng, L
AU  - Zheng L
AD  - Department of Comprehensive Laboratory, the Third Affiliated Hospital of Soochow 
      University, Changzhou 213003, China.
FAU - Zhang, X
AU  - Zhang X
AD  - Cardiothoracic Surgery, the Third Affiliated Hospital of Soochow University, 
      Changzhou 213003, China.
FAU - Xu, N
AU  - Xu N
AD  - Division of Clinical Chemistry and Pharmacology, Department of Laboratory 
      Medicine, Lunds University, S-221 85 Lund, Sweden.
FAU - Luo, G
AU  - Luo G
AD  - Department of Comprehensive Laboratory, the Third Affiliated Hospital of Soochow 
      University, Changzhou 213003, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Curr Mol Med
JT  - Current molecular medicine
JID - 101093076
RN  - 0 (Apolipoproteins M)
RN  - 0 (Lipoproteins, HDL)
RN  - 0 (RNA, Messenger)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - EC 2.7.11.1 (AKT1 protein, human)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Apolipoproteins M/genetics/*metabolism
MH  - Case-Control Studies
MH  - Female
MH  - Hep G2 Cells
MH  - Homocysteine/*blood
MH  - Humans
MH  - Hyperhomocysteinemia/blood/genetics/*pathology
MH  - Lipoproteins, HDL/blood
MH  - Male
MH  - Middle Aged
MH  - Phosphatidylinositol 3-Kinases/genetics/metabolism
MH  - Proto-Oncogene Proteins c-akt/genetics/metabolism
MH  - RNA, Messenger/genetics/*metabolism
OTO - NOTNLM
OT  - Homocysteine
OT  - PI3K
OT  - apolipoprotein M
OT  - cardiovascular diseases
OT  - hyperhomocysteinemia
OT  - lipid metabolism.
EDAT- 2019/03/12 06:00
MHDA- 2020/06/10 06:00
CRDT- 2019/03/12 06:00
PHST- 2018/09/30 00:00 [received]
PHST- 2019/02/20 00:00 [revised]
PHST- 2019/02/22 00:00 [accepted]
PHST- 2019/03/12 06:00 [pubmed]
PHST- 2020/06/10 06:00 [medline]
PHST- 2019/03/12 06:00 [entrez]
AID - CMM-EPUB-97174 [pii]
AID - 10.2174/1566524019666190308115624 [doi]
PST - ppublish
SO  - Curr Mol Med. 2019;19(2):120-126. doi: 10.2174/1566524019666190308115624.