PMID- 30858979
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230106
IS  - 2052-2975 (Print)
IS  - 2052-2975 (Electronic)
IS  - 2052-2975 (Linking)
VI  - 29
DP  - 2019 May
TI  - Identification of relevant regions on structural and nonstructural proteins of 
      Zika virus for vaccine and diagnostic test development: an in silico approach.
PG  - 100506
LID - 10.1016/j.nmni.2019.01.002 [doi]
LID - 100506
AB  - Zika virus (ZIKV) is an arbovirus belonging to the Flaviviridae family and the 
      genus Flavivirus. Infection with ZIKV causes a mild, self-limiting febrile 
      illness called Zika fever. However, ZIKV infection has been recently associated 
      with microcephaly and Guillain-Barre syndrome. Vaccines for the disease are a 
      high priority of World Health Organization. Several studies are currently being 
      conducted to develop a vaccine against ZIKV, but until now there is no licensed 
      ZIKV vaccine. This study used a novel immunoinformatics approach to identify 
      potential T-cell immunogenic epitopes present in the structural and nonstructural 
      proteins of ZIKV. Fourteen T-cell candidate epitopes were identified on ZIKV 
      structural and nonstructural proteins: pr(36-50); C(61-75); C(103-117); 
      E(374-382); E(477-491); NS2a(90-104); NS2a(174-188); NS2a(179-193); 
      NS2a(190-204); NS2a(195-209); NS2a(200-214); NS3(175-189); and NS4a(82-96); 
      NS4a(99-113). Among these epitopes, only E(374-382) is a human leukocyte antigen 
      (HLA) type I restricted epitope. All identified epitopes showed a low similarity 
      with other important flaviviruses but had a high conservation rate among the ZIKV 
      strains and a high population coverage rate. Therefore, these predicted T-cell 
      epitopes are potential candidates targets for development of vaccines to prevent 
      ZIKV infection.
FAU - Salvador, E A
AU  - Salvador EA
AD  - Institute of Biomedical Sciences, Department of Microbiology and Immunology, 
      Federal University of Alfenas, Minas Gerais, Brazil.
FAU - Pires de Souza, G A
AU  - Pires de Souza GA
AD  - Institute of Biomedical Sciences, Department of Microbiology and Immunology, 
      Federal University of Alfenas, Minas Gerais, Brazil.
FAU - Cotta Malaquias, L C
AU  - Cotta Malaquias LC
AD  - Institute of Biomedical Sciences, Department of Microbiology and Immunology, 
      Federal University of Alfenas, Minas Gerais, Brazil.
FAU - Wang, T
AU  - Wang T
AD  - Department of Microbiology & Immunology, Department of Pathology, Center for 
      Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, 
      Galveston, TX, USA.
FAU - Leomil Coelho, L F
AU  - Leomil Coelho LF
AD  - Institute of Biomedical Sciences, Department of Microbiology and Immunology, 
      Federal University of Alfenas, Minas Gerais, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20190131
PL  - England
TA  - New Microbes New Infect
JT  - New microbes and new infections
JID - 101624750
PMC - PMC6396434
OTO - NOTNLM
OT  - Diagnostic test
OT  - Immunoinformatic
OT  - T-cell epitope
OT  - Zika virus
OT  - vaccine
EDAT- 2019/03/13 06:00
MHDA- 2019/03/13 06:01
PMCR- 2019/01/31
CRDT- 2019/03/13 06:00
PHST- 2018/10/28 00:00 [received]
PHST- 2019/01/11 00:00 [revised]
PHST- 2019/01/15 00:00 [accepted]
PHST- 2019/03/13 06:00 [entrez]
PHST- 2019/03/13 06:00 [pubmed]
PHST- 2019/03/13 06:01 [medline]
PHST- 2019/01/31 00:00 [pmc-release]
AID - S2052-2975(19)30002-2 [pii]
AID - 100506 [pii]
AID - 10.1016/j.nmni.2019.01.002 [doi]
PST - epublish
SO  - New Microbes New Infect. 2019 Jan 31;29:100506. doi: 10.1016/j.nmni.2019.01.002. 
      eCollection 2019 May.