PMID- 30859267
OWN - NLM
STAT- MEDLINE
DCOM- 20190424
LR  - 20191210
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 411
IP  - 11
DP  - 2019 Apr
TI  - Multi-residue analysis of plant growth regulators and pesticides in traditional 
      Chinese medicines by high-performance liquid chromatography coupled with tandem 
      mass spectrometry.
PG  - 2447-2460
LID - 10.1007/s00216-019-01691-8 [doi]
AB  - A multi-residue method was developed for the simultaneous determination of 24 
      plant growth regulators (PGRs) and 11 representative pesticides that were widely 
      applied in plants used in traditional Chinese medicines (TCMs) by 
      high-performance liquid chromatography coupled with tandem mass spectrometry 
      (HPLC-MS/MS). The method was validated taking into consideration EU guidelines; 
      calibration curves for all of the targeted analytes showed correlation 
      coefficients (gamma(2)) higher than 0.9901. The limits of detection (LOD) ranged from 
      0.2 to 8 mug/kg. The average recovery for all analytes in spiked samples ranged 
      from 63.18 to 127.23%, with a relative standard deviation of </= 15%. The proposed 
      method has been applied to 480 batches of TCM samples, including 34 species of 
      medicinal plants, from the TCM market. The results showed that 14 PGRs and 5 
      pesticides were detected, including choline chloride, chlormequat, paclobutrazol, 
      uniconazole, phoxim, etc. Among them, there were high detection rates for 
      chlormequat (40%), choline chloride (100%), atonik (73.75%), abscisic acid 
      (80.83%), and indole-3-acetic acid (41.25%). The residual level of paclobutrazol 
      in Ophiopogonis radix exceeded the recommended maximum residue limits (MRLs) 
      according to GB 2763-2016. In addition, 14 agrochemicals used in TCM planting 
      were collected and detected; the result showed various PGRs were detected in 
      samples registered as fertilizer. These results indicate that PGRs and pesticides 
      were widely used in the cultivation of medicinal plants, especially for radix and 
      rhizome herbs. The residue of targeted PGRs and pesticides in TCM samples from 
      this study have a high frequency and high level. Graphical abstract.
FAU - Luo, Zuliang
AU  - Luo Z
AD  - Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, 
      Peking Union Medical College, Beijing, 100193, China.
FAU - Zhang, Lixia
AU  - Zhang L
AD  - Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, 
      Peking Union Medical College, Beijing, 100193, China.
AD  - Yunnan Branch, Institute of Medicinal Plant Development, Chinese Academy of 
      Medical Sciences, Jing Hong, 666100, China.
FAU - Mou, Yan
AU  - Mou Y
AD  - Yunnan Branch, Institute of Medicinal Plant Development, Chinese Academy of 
      Medical Sciences, Jing Hong, 666100, China.
FAU - Cui, Shengrong
AU  - Cui S
AD  - Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, 
      Peking Union Medical College, Beijing, 100193, China.
FAU - Gu, Zhe
AU  - Gu Z
AD  - Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, 
      Peking Union Medical College, Beijing, 100193, China.
FAU - Yu, Jing
AU  - Yu J
AD  - Yunnan Branch, Institute of Medicinal Plant Development, Chinese Academy of 
      Medical Sciences, Jing Hong, 666100, China.
FAU - Ma, Xiaojun
AU  - Ma X
AD  - Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, 
      Peking Union Medical College, Beijing, 100193, China. mayixuan10@163.com.
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20190312
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Pesticide Residues)
RN  - 0 (Pesticides)
RN  - 0 (Plant Growth Regulators)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Drug Contamination
MH  - Drugs, Chinese Herbal/*analysis
MH  - Limit of Detection
MH  - Pesticide Residues/analysis
MH  - Pesticides/*analysis
MH  - Plant Growth Regulators/*analysis
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - HPLC-MS/MS
OT  - Multi-residue
OT  - Pesticides
OT  - Plant growth regulators
OT  - TCMs
EDAT- 2019/03/13 06:00
MHDA- 2019/04/25 06:00
CRDT- 2019/03/13 06:00
PHST- 2018/12/10 00:00 [received]
PHST- 2019/02/13 00:00 [accepted]
PHST- 2019/02/02 00:00 [revised]
PHST- 2019/03/13 06:00 [pubmed]
PHST- 2019/04/25 06:00 [medline]
PHST- 2019/03/13 06:00 [entrez]
AID - 10.1007/s00216-019-01691-8 [pii]
AID - 10.1007/s00216-019-01691-8 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2019 Apr;411(11):2447-2460. doi: 10.1007/s00216-019-01691-8. 
      Epub 2019 Mar 12.