PMID- 30860996
OWN - NLM
STAT- MEDLINE
DCOM- 20191210
LR  - 20200309
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 14
IP  - 3
DP  - 2019
TI  - Characterization of the Rosa roxburghii Tratt transcriptome and analysis of MYB 
      genes.
PG  - e0203014
LID - 10.1371/journal.pone.0203014 [doi]
LID - e0203014
AB  - Rosa roxburghii Tratt (Rosaceae) has a fruit that is flavorful, economically 
      valuable, and highly nutritious, providing numerous health benefits. 
      Myeloblastosis (MYB) proteins play key roles in the development and fruit quality 
      of R. roxburghii. However, there is little available genomic and transcriptomic 
      information for R. roxburghii. Here, a normalized cDNA library was constructed 
      from five tissues, including the stem, leaf, flower, young fruit, and mature 
      fruit, using the Illumina HiSeq 3000 platform. De novo assembly was performed, 
      and 470.66 million clean reads were obtained. In total, 63,727 unigenes, with an 
      average GC content of 42.08%, were discovered, 60,406 of which were annotated. In 
      addition, 9,354 unigenes were assigned to Gene Ontology categories, and 20,202 
      unigenes were assigned to 25 Eukaryotic Ortholog Groups. Additionally, 19,508 
      unigenes were classified into 140 pathways of the Kyoto Encyclopedia of Genes and 
      Genomes database. Based on the transcriptome, 163 unigenes associated with MYBs 
      were detected. Among these genes, 75 genes were significantly expressed in the 
      various tissues, including 10 R1 MYB, 42 R2R3 MYB, one R1R2R3 MYB, three R4 MYB 
      and 19 atypical MYB-like proteins. The expression levels of the 12 MYB genes 
      randomly selected for quantitative real-time PCR analysis corroborated the RNA 
      sequencing results. A total of 37,545 microsatellites were detected, with an 
      average expressed sequence tag-simple sequence repeat frequency of 0.59 
      (37,545/63,727). This transcriptome data improves our understanding of the role 
      of MYB in R. roxburghii and will be valuable for identifying genes of interest.
FAU - Huang, Xiaolong
AU  - Huang X
AD  - Key Laboratory of State Forestry Administration on Biodiversity Conservation in 
      Karst Mountainous Areas of Southwestern China /Key Laboratory of Plant Physiology 
      and Developmental Regulation/ School of Life Sciences, Guizhou Normal University, 
      Guiyang, China.
FAU - Yan, Huiqing
AU  - Yan H
AUID- ORCID: 0000-0001-9333-6336
AD  - School of Life Sciences, Guizhou Normal University, Guiyang, China.
FAU - Zhai, Lisheng
AU  - Zhai L
AD  - Key Laboratory of State Forestry Administration on Biodiversity Conservation in 
      Karst Mountainous Areas of Southwestern China /Key Laboratory of Plant Physiology 
      and Developmental Regulation/ School of Life Sciences, Guizhou Normal University, 
      Guiyang, China.
FAU - Yang, Zhengting
AU  - Yang Z
AD  - Key Laboratory of State Forestry Administration on Biodiversity Conservation in 
      Karst Mountainous Areas of Southwestern China /Key Laboratory of Plant Physiology 
      and Developmental Regulation/ School of Life Sciences, Guizhou Normal University, 
      Guiyang, China.
FAU - Yi, Yin
AU  - Yi Y
AD  - Key Laboratory of State Forestry Administration on Biodiversity Conservation in 
      Karst Mountainous Areas of Southwestern China /Key Laboratory of Plant Physiology 
      and Developmental Regulation/ School of Life Sciences, Guizhou Normal University, 
      Guiyang, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190312
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Plant Proteins)
RN  - 0 (RNA, Plant)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - China
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Plant
MH  - *Genes, Plant
MH  - Genes, myb
MH  - Microsatellite Repeats
MH  - Molecular Sequence Annotation
MH  - Plant Proteins/genetics
MH  - RNA, Plant/genetics/metabolism
MH  - Rosa/*genetics/metabolism
MH  - Tissue Distribution
MH  - Transcription Factors/genetics
PMC - PMC6414006
COIS- The authors have declared that no competing interests exist.
EDAT- 2019/03/13 06:00
MHDA- 2019/12/18 06:00
PMCR- 2019/03/12
CRDT- 2019/03/13 06:00
PHST- 2018/08/09 00:00 [received]
PHST- 2019/02/16 00:00 [accepted]
PHST- 2019/03/13 06:00 [entrez]
PHST- 2019/03/13 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/03/12 00:00 [pmc-release]
AID - PONE-D-18-23126 [pii]
AID - 10.1371/journal.pone.0203014 [doi]
PST - epublish
SO  - PLoS One. 2019 Mar 12;14(3):e0203014. doi: 10.1371/journal.pone.0203014. 
      eCollection 2019.