PMID- 30862559
OWN - NLM
STAT- MEDLINE
DCOM- 20190528
LR  - 20190528
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 130
DP  - 2019 May
TI  - Changes of T lymphocyte subsets in patients with HIV-negative symptomatic 
      neurosyphilis.
PG  - 213-218
LID - S0882-4010(18)31333-0 [pii]
LID - 10.1016/j.micpath.2019.03.008 [doi]
AB  - OBJECTIVES: The host immune response could be an imperative factor in the 
      pathogenesis of neurosyphilis, but the role of T lymphocyte subsets remains 
      unclear. In the present study, we assessed the CD4(+) T and CD8(+) T cell subsets 
      in the peripheral blood of patients with HIV-negative symptomatic neurosyphilis 
      and then explored the clinical application value of neurosyphilis. METHODS: In 
      total, 24 patients with HIV-negative symptomatic neurosyphilis and 22 patients 
      with syphilis/non-neurosyphilis were included in this study and cerebrospinal 
      fluid (CSF) and blood samples were obtained. Th1, Th2, Th17, Th9, CD8+IFN-gamma+, 
      CD8+IL-4+, CD8+IL-9+, and CD8+IL-17 + cells were identified by flow cytometry. 
      RESULTS: The levels of CD8+IFN-gamma+ were significantly increased in the peripheral 
      blood of neurosyphilis patients compared to that in syphilis/non-neurosyphilis 
      patients, but it was opposite to Th2, Th9, CD8+IL-4+, CD8+IL-9+, and 
      CD8+IL-17 + cells. Dendritic cells (DCs) of neurosyphilis matured by T. pallidum 
      induced the development of a combination of IFN-gamma-producing Th1 cells. The number 
      of CD8+IL-17 + cells was significantly correlated with the CSF RPR and CSF TPPA 
      levels. ROC curve analysis revealed that the number of CD8+IFN-gamma+ cells could be 
      a potential biomarker for neurosyphilis from non-neurosyphilis/syphilis. 
      CONCLUSIONS: High expression of CD8+IFN-gamma+ cells and low expression of 
      CD8+IL-17 + cells in patients with symptomatic neurosyphilis, which explains the 
      pathogenesis of symptomatic neurosyphilis, meanwhile CD8+IFN-gamma+ cells may be a 
      better indicator in classifying symptomatic neurosyphilis from 
      non-neurosyphilis/syphilis among patients without HIV infection.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Liu, Li-Li
AU  - Liu LL
AD  - Center of Clinical Laboratory, Zhongshan Hospital, School of Medical, Xiamen 
      University, Xiamen, Fujian Province, China; Institute of Infectious Disease, 
      School of Medicine, Xiamen University, Xiamen, Fujian Province, China.
FAU - Liu, Wen-Na
AU  - Liu WN
AD  - Center of Clinical Laboratory, Zhongshan Hospital, School of Medical, Xiamen 
      University, Xiamen, Fujian Province, China.
FAU - Jiang, Xiao-Yong
AU  - Jiang XY
AD  - Department of Dermatology, Zhongshan Hospital, School of Medical, Xiamen 
      University, Xiamen, Fujian Province, China.
FAU - Jun-Ren
AU  - Jun-Ren
AD  - Department of Dermatology, Zhongshan Hospital, School of Medical, Xiamen 
      University, Xiamen, Fujian Province, China.
FAU - Chen, Mei-Hua
AU  - Chen MH
AD  - Department of Dermatology, Zhongshan Hospital, School of Medical, Xiamen 
      University, Xiamen, Fujian Province, China.
FAU - Liu, Zhao-Ji
AU  - Liu ZJ
AD  - Department of Neurology, Zhongshan Hospital, School of Medical, Xiamen 
      University, Xiamen, Fujian Province, China.
FAU - Lin, Yong
AU  - Lin Y
AD  - Center of Clinical Laboratory, Zhongshan Hospital, School of Medical, Xiamen 
      University, Xiamen, Fujian Province, China.
FAU - Zhu, Shao-Gang
AU  - Zhu SG
AD  - Center of Clinical Laboratory, Zhongshan Hospital, School of Medical, Xiamen 
      University, Xiamen, Fujian Province, China.
FAU - Lin, Li-Rong
AU  - Lin LR
AD  - Center of Clinical Laboratory, Zhongshan Hospital, School of Medical, Xiamen 
      University, Xiamen, Fujian Province, China.
FAU - Zheng, Wei-Hong
AU  - Zheng WH
AD  - Department of Neurology, Zhongshan Hospital, School of Medical, Xiamen 
      University, Xiamen, Fujian Province, China.
FAU - Yan, Jiang-Hua
AU  - Yan JH
AD  - Cancer Research Center, School of Medical, Xiamen University, Xiamen, Fujian 
      Province, China. Electronic address: jhyan@xmu.edu.cn.
FAU - Yang, Tian-Ci
AU  - Yang TC
AD  - Center of Clinical Laboratory, Zhongshan Hospital, School of Medical, Xiamen 
      University, Xiamen, Fujian Province, China. Electronic address: 
      yangtianci@xmu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190309
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
SB  - IM
MH  - Adult
MH  - Aged
MH  - Blood Cells
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Cerebrospinal Fluid/cytology
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Neurosyphilis/*pathology
MH  - T-Lymphocyte Subsets/*immunology
OTO - NOTNLM
OT  - CD8+IFN-gamma+ cells
OT  - CD8+IL-17+ cells
OT  - Neurosyphilis
OT  - Th2 cells
EDAT- 2019/03/14 06:00
MHDA- 2019/05/29 06:00
CRDT- 2019/03/14 06:00
PHST- 2018/07/27 00:00 [received]
PHST- 2019/01/31 00:00 [revised]
PHST- 2019/03/06 00:00 [accepted]
PHST- 2019/03/14 06:00 [pubmed]
PHST- 2019/05/29 06:00 [medline]
PHST- 2019/03/14 06:00 [entrez]
AID - S0882-4010(18)31333-0 [pii]
AID - 10.1016/j.micpath.2019.03.008 [doi]
PST - ppublish
SO  - Microb Pathog. 2019 May;130:213-218. doi: 10.1016/j.micpath.2019.03.008. Epub 
      2019 Mar 9.