PMID- 30862918
OWN - NLM
STAT- MEDLINE
DCOM- 20200930
LR  - 20210109
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Mar 12
TI  - Contrasting in vitro and in vivo methanol oxidation activities of 
      lanthanide-dependent alcohol dehydrogenases XoxF1 and ExaF from Methylobacterium 
      extorquens AM1.
PG  - 4248
LID - 10.1038/s41598-019-41043-1 [doi]
LID - 4248
AB  - Lanthanide (Ln) elements are utilized as cofactors for catalysis by XoxF-type 
      methanol dehydrogenases (MDHs). A primary assumption is that XoxF enzymes produce 
      formate from methanol oxidation, which could impact organisms that require 
      formaldehyde for assimilation. We report genetic and phenotypic evidence showing 
      that XoxF1 (MexAM1_1740) from Methylobacterium extorquens AM1 produces 
      formaldehyde, and not formate, during growth with methanol. Enzyme purified with 
      lanthanum or neodymium oxidizes formaldehyde. However, formaldehyde oxidation via 
      2,6-dichlorophenol-indophenol (DCPIP) reduction is not detected in cell-free 
      extracts from wild-type strain methanol- and lanthanum-grown cultures. 
      Formaldehyde activating enzyme (Fae) is required for Ln methylotrophic growth, 
      demonstrating that XoxF1-mediated production of formaldehyde is essential. 
      Addition of exogenous lanthanum increases growth rate with methanol by 9-12% but 
      does not correlate with changes to methanol consumption or formaldehyde 
      accumulation. Transcriptomics analysis of lanthanum methanol growth shows 
      upregulation of xox1 and downregulation of mxa genes, consistent with the 
      Ln-switch, no differential expression of formaldehyde conversion genes, 
      downregulation of pyrroloquinoline quinone (PQQ) biosynthesis genes, and 
      upregulation of fdh4 formate dehydrogenase (FDH) genes. Additionally, the 
      Ln-dependent ethanol dehydrogenase ExaF reduces methanol sensitivity in the fae 
      mutant strain when lanthanides are present, providing evidence for the capacity 
      of an auxiliary role for ExaF during Ln-dependent methylotrophy.
FAU - Good, Nathan M
AU  - Good NM
AD  - Martinez-Gomez laboratory, Department of Microbiology and Molecular Genetics, 
      Michigan State University, East Lansing, MI, USA.
FAU - Moore, Riley S
AU  - Moore RS
AD  - Martinez-Gomez laboratory, Department of Microbiology and Molecular Genetics, 
      Michigan State University, East Lansing, MI, USA.
FAU - Suriano, Carly J
AU  - Suriano CJ
AD  - Martinez-Gomez laboratory, Department of Microbiology and Molecular Genetics, 
      Michigan State University, East Lansing, MI, USA.
FAU - Martinez-Gomez, N Cecilia
AU  - Martinez-Gomez NC
AD  - Martinez-Gomez laboratory, Department of Microbiology and Molecular Genetics, 
      Michigan State University, East Lansing, MI, USA. mart1754@msu.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20190312
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Bacterial Proteins)
RN  - 0 (Coenzymes)
RN  - 0 (Lanthanoid Series Elements)
RN  - 1HG84L3525 (Formaldehyde)
RN  - EC 1.1.- (Alcohol Oxidoreductases)
RN  - EC 1.1.2.8 (alcohol dehydrogenase (acceptor))
RN  - EC 1.17.1.9 (Formate Dehydrogenases)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Alcohol Oxidoreductases/genetics/*metabolism
MH  - Bacterial Proteins/genetics/*metabolism
MH  - Biocatalysis
MH  - Biosynthetic Pathways/genetics
MH  - Coenzymes/metabolism
MH  - Enzyme Assays
MH  - Formaldehyde/metabolism
MH  - Formate Dehydrogenases/genetics/metabolism
MH  - Gene Expression Profiling
MH  - Genes, Bacterial/genetics
MH  - Lanthanoid Series Elements/*metabolism
MH  - Methanol/*metabolism
MH  - Methylobacterium extorquens/*enzymology/genetics
MH  - Oxidation-Reduction
PMC - PMC6414531
COIS- The authors declare no competing interests.
EDAT- 2019/03/14 06:00
MHDA- 2020/10/02 06:00
PMCR- 2019/03/12
CRDT- 2019/03/14 06:00
PHST- 2018/10/22 00:00 [received]
PHST- 2019/02/28 00:00 [accepted]
PHST- 2019/03/14 06:00 [entrez]
PHST- 2019/03/14 06:00 [pubmed]
PHST- 2020/10/02 06:00 [medline]
PHST- 2019/03/12 00:00 [pmc-release]
AID - 10.1038/s41598-019-41043-1 [pii]
AID - 41043 [pii]
AID - 10.1038/s41598-019-41043-1 [doi]
PST - epublish
SO  - Sci Rep. 2019 Mar 12;9(1):4248. doi: 10.1038/s41598-019-41043-1.