PMID- 30865898
OWN - NLM
STAT- MEDLINE
DCOM- 20200415
LR  - 20231213
IS  - 2211-1247 (Electronic)
VI  - 26
IP  - 11
DP  - 2019 Mar 12
TI  - Single-Cell Analysis Reveals Heterogeneity of High Endothelial Venules and 
      Different Regulation of Genes Controlling Lymphocyte Entry to Lymph Nodes.
PG  - 3116-3131.e5
LID - S2211-1247(19)30213-X [pii]
LID - 10.1016/j.celrep.2019.02.042 [doi]
AB  - High-endothelial venules (HEVs) are specialized blood vessels allowing 
      recirculation of naive lymphocytes through lymphoid organs. Here, using 
      full-length, single-cell RNA sequencing, RNA fluorescence in situ hybridization 
      (FISH), flow cytometry, and immunohistofluorescence, we reveal the heterogeneity 
      of HEVs in adult mouse peripheral lymph nodes (PLNs) under conditions of 
      homeostasis, antigenic stimulation, and after inhibition of lymphotoxin-beta 
      receptor (LTbetaR) signaling. We demonstrate that HEV endothelial cells are in an 
      activated state during homeostasis, and we identify the genes characteristic of 
      the differentiated HEV phenotype. We show that LTbetaR signaling regulates many HEV 
      genes and pathways in resting PLNs and that immune stimulation induces a global 
      and temporary inflammatory phenotype in HEVs without compromising their ability 
      to recruit naive lymphocytes. Most importantly, we uncover differences in the 
      regulation of genes controlling lymphocyte trafficking, Glycam1, Fut7, Gcnt1, 
      Chst4, B3gnt3, and Ccl21a, that have implications for HEV function and regulation 
      in health and disease.
CI  - Copyright (c) 2019 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Veerman, Krystle
AU  - Veerman K
AD  - Institut de Pharmacologie et de Biologie Structurale (IPBS), Universite de 
      Toulouse, CNRS, UPS, Toulouse, France.
FAU - Tardiveau, Claire
AU  - Tardiveau C
AD  - Institut de Pharmacologie et de Biologie Structurale (IPBS), Universite de 
      Toulouse, CNRS, UPS, Toulouse, France.
FAU - Martins, Frederic
AU  - Martins F
AD  - Institut des Maladies Metaboliques et Cardiovasculaires (I2MC), UMR1048, INSERM, 
      UPS, Toulouse, France; Plateforme Genome et Transcriptome (GeT), Genopole 
      Toulouse, Toulouse, France.
FAU - Coudert, Juliette
AU  - Coudert J
AD  - Institut de Pharmacologie et de Biologie Structurale (IPBS), Universite de 
      Toulouse, CNRS, UPS, Toulouse, France.
FAU - Girard, Jean-Philippe
AU  - Girard JP
AD  - Institut de Pharmacologie et de Biologie Structurale (IPBS), Universite de 
      Toulouse, CNRS, UPS, Toulouse, France. Electronic address: 
      jean-philippe.girard@ipbs.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Rep
JT  - Cell reports
JID - 101573691
RN  - 0 (Ccl21a protein, mouse)
RN  - 0 (Chemokine CCL21)
RN  - 0 (GCN1 protein, mouse)
RN  - 0 (Ltbr protein, mouse)
RN  - 0 (Lymphotoxin beta Receptor)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Trans-Activators)
RN  - EC 2.4.1.- (Fucosyltransferases)
RN  - EC 2.4.1.- (N-Acetylglucosaminyltransferases)
RN  - EC 2.4.1.- (fucosyltransferase VII, mouse)
RN  - EC 2.8.2.- (Sulfotransferases)
SB  - IM
MH  - Animals
MH  - Cell Movement/*genetics
MH  - Chemokine CCL21/genetics/metabolism
MH  - Endothelium, Vascular/cytology/*metabolism
MH  - Female
MH  - Fucosyltransferases/genetics/metabolism
MH  - Genetic Heterogeneity
MH  - *Homeostasis
MH  - Lymph Nodes/cytology
MH  - Lymphocytes/metabolism/*physiology
MH  - Lymphotoxin beta Receptor/genetics/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - N-Acetylglucosaminyltransferases/genetics/metabolism
MH  - RNA-Binding Proteins/genetics/metabolism
MH  - Single-Cell Analysis
MH  - Sulfotransferases/genetics/metabolism
MH  - Trans-Activators/genetics/metabolism
MH  - *Transcriptome
MH  - Venules/cytology/*metabolism
MH  - Carbohydrate Sulfotransferases
OTO - NOTNLM
OT  - endothelial cell
OT  - high-endothelial venule
OT  - homeostasis
OT  - inflammation
OT  - lymphocyte homing
OT  - lymphocyte trafficking
OT  - lymphotoxin-beta receptor
OT  - peripheral lymph node
OT  - scRNA-seq
OT  - single-cell RNA sequencing
EDAT- 2019/03/14 06:00
MHDA- 2020/04/16 06:00
CRDT- 2019/03/14 06:00
PHST- 2018/12/05 00:00 [received]
PHST- 2019/01/25 00:00 [revised]
PHST- 2019/02/11 00:00 [accepted]
PHST- 2019/03/14 06:00 [entrez]
PHST- 2019/03/14 06:00 [pubmed]
PHST- 2020/04/16 06:00 [medline]
AID - S2211-1247(19)30213-X [pii]
AID - 10.1016/j.celrep.2019.02.042 [doi]
PST - ppublish
SO  - Cell Rep. 2019 Mar 12;26(11):3116-3131.e5. doi: 10.1016/j.celrep.2019.02.042.