PMID- 30867482
OWN - NLM
STAT- MEDLINE
DCOM- 20201020
LR  - 20210109
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Mar 13
TI  - Rg6, a rare ginsenoside, inhibits systemic inflammation through the induction of 
      interleukin-10 and microRNA-146a.
PG  - 4342
LID - 10.1038/s41598-019-40690-8 [doi]
LID - 4342
AB  - The immunobiological functions of Rg6, a rare ginsenoside from ginseng, have been 
      largely unreported. In this paper, we demonstrate that Rg6 has a significant 
      immunosuppressive function on Toll-like receptor (TLR) 4-induced systemic 
      inflammatory responses. Rg6 was found to negatively regulate pro-inflammatory 
      responses and severity in vivo, and thus induced recovery in mice with 
      lipopolysaccharide (LPS)-induced septic shock and cecal ligation and puncture 
      (CLP)-induced sepsis. Rg6 treatment also facilitated recovery in mice with 
      LPS-induced lung damage via reduced neutrophil infiltration and tumor necrosis 
      factor-alpha expression in lung tissues. Rg6 injection also downregulated 
      pro-inflammatory cytokines and increased the levels of interleukin (IL)-10 in the 
      serum of septic mice. Mechanistically, Rg6 did not induce TLR negative 
      regulators, such as A20 and IRAK-M, in bone marrow-derived macrophages (BMDMs). 
      Instead, addition of Rg6 to LPS-activated BMDMs augmented IL-10 expression, 
      whereas it inhibited inflammatory signaling, such as by nuclear factor kappaB 
      activation and mitogen-activated protein kinases. Furthermore, Rg6 significantly 
      induced miR-146a, an operator miRNA for anti-inflammation, in BMDMs. 
      Collectively, these data indicate that Rg6 inhibits inflammatory responses 
      through the induction of IL-10 and miR-146a.
FAU - Paik, Seungwha
AU  - Paik S
AD  - Department of Microbiology, Chungnam National University School of Medicine, 
      Daejeon, 35015, Republic of Korea.
AD  - Department of Medical Science, Chungnam National University School of Medicine, 
      Daejeon, 35015, Republic of Korea.
AD  - Infection Control Convergence Research Center, Chungnam National University, 
      Daejeon, 35015, Republic of Korea.
FAU - Choe, Jin Ho
AU  - Choe JH
AD  - Department of Microbiology, Chungnam National University School of Medicine, 
      Daejeon, 35015, Republic of Korea.
AD  - Department of Medical Science, Chungnam National University School of Medicine, 
      Daejeon, 35015, Republic of Korea.
AD  - Infection Control Convergence Research Center, Chungnam National University, 
      Daejeon, 35015, Republic of Korea.
FAU - Choi, Ga-Eun
AU  - Choi GE
AD  - Infection Control Convergence Research Center, Chungnam National University, 
      Daejeon, 35015, Republic of Korea.
AD  - College of Pharmacy, Chungnam National University, Daejeon, 34134, Republic of 
      Korea.
FAU - Kim, Ji-Eun
AU  - Kim JE
AD  - Infection Control Convergence Research Center, Chungnam National University, 
      Daejeon, 35015, Republic of Korea.
AD  - College of Pharmacy, Chungnam National University, Daejeon, 34134, Republic of 
      Korea.
FAU - Kim, Jin-Man
AU  - Kim JM
AD  - Department of Medical Science, Chungnam National University School of Medicine, 
      Daejeon, 35015, Republic of Korea.
AD  - Infection Control Convergence Research Center, Chungnam National University, 
      Daejeon, 35015, Republic of Korea.
AD  - Department of Pathology, Chungnam National University School of Medicine, 
      Daejeon, 35015, Republic of Korea.
FAU - Song, Gyu Yong
AU  - Song GY
AD  - Infection Control Convergence Research Center, Chungnam National University, 
      Daejeon, 35015, Republic of Korea. gysong@cnu.ac.kr.
AD  - College of Pharmacy, Chungnam National University, Daejeon, 34134, Republic of 
      Korea. gysong@cnu.ac.kr.
FAU - Jo, Eun-Kyeong
AU  - Jo EK
AD  - Department of Microbiology, Chungnam National University School of Medicine, 
      Daejeon, 35015, Republic of Korea. hayoungj@cnu.ac.kr.
AD  - Department of Medical Science, Chungnam National University School of Medicine, 
      Daejeon, 35015, Republic of Korea. hayoungj@cnu.ac.kr.
AD  - Infection Control Convergence Research Center, Chungnam National University, 
      Daejeon, 35015, Republic of Korea. hayoungj@cnu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190313
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Ginsenosides)
RN  - 0 (IL10 protein, mouse)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn146 microRNA, mouse)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - Ginsenosides/*pharmacology
MH  - Inflammation/*prevention & control
MH  - Interleukin-10/*biosynthesis
MH  - Lipopolysaccharides/toxicity
MH  - Macrophages/metabolism
MH  - Mice
MH  - MicroRNAs/*biosynthesis
MH  - Sepsis/chemically induced/etiology/metabolism/prevention & control
MH  - Survival Rate
PMC - PMC6416268
COIS- The authors declare no competing interests.
EDAT- 2019/03/15 06:00
MHDA- 2020/10/21 06:00
PMCR- 2019/03/13
CRDT- 2019/03/15 06:00
PHST- 2018/10/12 00:00 [received]
PHST- 2019/02/15 00:00 [accepted]
PHST- 2019/03/15 06:00 [entrez]
PHST- 2019/03/15 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
PHST- 2019/03/13 00:00 [pmc-release]
AID - 10.1038/s41598-019-40690-8 [pii]
AID - 40690 [pii]
AID - 10.1038/s41598-019-40690-8 [doi]
PST - epublish
SO  - Sci Rep. 2019 Mar 13;9(1):4342. doi: 10.1038/s41598-019-40690-8.