PMID- 30870663
OWN - NLM
STAT- MEDLINE
DCOM- 20190509
LR  - 20190509
IS  - 1879-1514 (Electronic)
IS  - 0166-445X (Linking)
VI  - 210
DP  - 2019 May
TI  - Seawater acidification increases copper toxicity: A multi-biomarker approach with 
      a key marine invertebrate, the Pacific Oyster Crassostrea gigas.
PG  - 167-178
LID - S0166-445X(18)31028-2 [pii]
LID - 10.1016/j.aquatox.2019.03.002 [doi]
AB  - Ocean acidification (OA) has been found to increase the release of free Cu(2+) in 
      seawater. However, only a handful of studies have investigated the influence of 
      OA on Cu accumulation and cellular toxicity in bivalve species. In this study, 
      Pacific oysters, Crassostrea gigas, were exposed to 25 mug/L Cu(2+) at three pH 
      levels (8.1, 7.8 and 7.6) for 14 and 28 days. Physiological and histopathological 
      parameters [(clearance rate (CR), respiration rate (RR), histopathological damage 
      and condition index (CI)), oxidative stress and neurotoxicity biomarkers 
      [superoxide dismutase (SOD) and glutathione transferase (GST) activities, lipid 
      peroxidation (LPO) and acetylcholinesterase (AChE) activity], combined with 
      glycolytic enzyme activities [pyruvate kinase (PK) and hexokinase (HK)] were 
      investigated in C. gigas. The bioconcentration of Cu was increased in soft 
      tissues of Cu-exposed oysters under OA. Our results suggest that both OA and Cu 
      could lead to physiological disturbance, oxidative stress, cellular damage, 
      disturbance in energy metabolism and neurotoxicity in oysters. The inhibited CR, 
      increased glycolytic enzymes activities and decreased CI suggested that the 
      energy metabolism strategy adopted by oysters was not sustainable in the long 
      term. Furthermore, integrated biomarker response (IBR) results found that OA and 
      Cu exposure lead to severe stress to oysters, and co-exposure was the most 
      stressful condition. Results from this study highlight the need to include OA in 
      future environmental assessments of pollutants and hazardous materials to better 
      elucidate the risks of those environmental perturbations.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Cao, Ruiwen
AU  - Cao R
AD  - Muping Coastal Environmental Research Station, Yantai Institute of Coastal Zone 
      Research, Chinese Academy of Sciences, Yantai, Shandong, 264117, PR China; Center 
      for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, Shandong, 266071, 
      PR China; Key Laboratory of Coastal Biology and Biological Resources Utilization, 
      Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 
      Shandong, 264003, PR China; University of Chinese Academy of Sciences, Beijing, 
      100049, PR China.
FAU - Zhang, Tianyu
AU  - Zhang T
AD  - Muping Coastal Environmental Research Station, Yantai Institute of Coastal Zone 
      Research, Chinese Academy of Sciences, Yantai, Shandong, 264117, PR China; Center 
      for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, Shandong, 266071, 
      PR China; Key Laboratory of Coastal Biology and Biological Resources Utilization, 
      Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 
      Shandong, 264003, PR China; University of Chinese Academy of Sciences, Beijing, 
      100049, PR China.
FAU - Li, Xiao
AU  - Li X
AD  - Muping Coastal Environmental Research Station, Yantai Institute of Coastal Zone 
      Research, Chinese Academy of Sciences, Yantai, Shandong, 264117, PR China; Center 
      for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, Shandong, 266071, 
      PR China; Key Laboratory of Coastal Biology and Biological Resources Utilization, 
      Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 
      Shandong, 264003, PR China; University of Chinese Academy of Sciences, Beijing, 
      100049, PR China.
FAU - Zhao, Yuting
AU  - Zhao Y
AD  - Shandong Marine Resource and Environment Research Institute, Yantai, 264006, PR 
      China.
FAU - Wang, Qing
AU  - Wang Q
AD  - Muping Coastal Environmental Research Station, Yantai Institute of Coastal Zone 
      Research, Chinese Academy of Sciences, Yantai, Shandong, 264117, PR China; Center 
      for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, Shandong, 266071, 
      PR China.
FAU - Yang, Dinglong
AU  - Yang D
AD  - Muping Coastal Environmental Research Station, Yantai Institute of Coastal Zone 
      Research, Chinese Academy of Sciences, Yantai, Shandong, 264117, PR China; Center 
      for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, Shandong, 266071, 
      PR China.
FAU - Qu, Yi
AU  - Qu Y
AD  - Muping Coastal Environmental Research Station, Yantai Institute of Coastal Zone 
      Research, Chinese Academy of Sciences, Yantai, Shandong, 264117, PR China; Center 
      for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, Shandong, 266071, 
      PR China; Key Laboratory of Coastal Biology and Biological Resources Utilization, 
      Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 
      Shandong, 264003, PR China.
FAU - Liu, Hui
AU  - Liu H
AD  - Muping Coastal Environmental Research Station, Yantai Institute of Coastal Zone 
      Research, Chinese Academy of Sciences, Yantai, Shandong, 264117, PR China; Center 
      for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, Shandong, 266071, 
      PR China.
FAU - Dong, Zhijun
AU  - Dong Z
AD  - Muping Coastal Environmental Research Station, Yantai Institute of Coastal Zone 
      Research, Chinese Academy of Sciences, Yantai, Shandong, 264117, PR China; Center 
      for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, Shandong, 266071, 
      PR China.
FAU - Zhao, Jianmin
AU  - Zhao J
AD  - Muping Coastal Environmental Research Station, Yantai Institute of Coastal Zone 
      Research, Chinese Academy of Sciences, Yantai, Shandong, 264117, PR China; Center 
      for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, Shandong, 266071, 
      PR China; Key Laboratory of Coastal Biology and Biological Resources Utilization, 
      Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 
      Shandong, 264003, PR China. Electronic address: jmzhao@yic.ac.cn.
LA  - eng
PT  - Journal Article
DEP - 20190305
PL  - Netherlands
TA  - Aquat Toxicol
JT  - Aquatic toxicology (Amsterdam, Netherlands)
JID - 8500246
RN  - 0 (Biomarkers)
RN  - 0 (Water Pollutants, Chemical)
RN  - 789U1901C5 (Copper)
SB  - IM
MH  - Animals
MH  - Biomarkers/metabolism
MH  - China
MH  - Copper/*toxicity
MH  - Crassostrea/*drug effects/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Energy Metabolism/drug effects
MH  - Hydrogen-Ion Concentration
MH  - Lipid Peroxidation/drug effects
MH  - Oceans and Seas
MH  - Oxidative Stress/drug effects
MH  - Seawater/*chemistry
MH  - Water Pollutants, Chemical/*toxicity
OTO - NOTNLM
OT  - Bioconcentration
OT  - Copper
OT  - Crassostrea gigas
OT  - Multi-biomarker
OT  - Ocean acidification
OT  - Oxidative stress
EDAT- 2019/03/15 06:00
MHDA- 2019/05/10 06:00
CRDT- 2019/03/15 06:00
PHST- 2018/11/19 00:00 [received]
PHST- 2019/02/22 00:00 [revised]
PHST- 2019/03/04 00:00 [accepted]
PHST- 2019/03/15 06:00 [pubmed]
PHST- 2019/05/10 06:00 [medline]
PHST- 2019/03/15 06:00 [entrez]
AID - S0166-445X(18)31028-2 [pii]
AID - 10.1016/j.aquatox.2019.03.002 [doi]
PST - ppublish
SO  - Aquat Toxicol. 2019 May;210:167-178. doi: 10.1016/j.aquatox.2019.03.002. Epub 
      2019 Mar 5.