PMID- 30870695
OWN - NLM
STAT- MEDLINE
DCOM- 20190711
LR  - 20190711
IS  - 1872-7077 (Electronic)
IS  - 1382-6689 (Linking)
VI  - 68
DP  - 2019 May
TI  - Melatonin alleviates beta-zearalenol and HT-2 toxin-induced apoptosis and oxidative 
      stress in bovine ovarian granulosa cells.
PG  - 52-60
LID - S1382-6689(18)30398-3 [pii]
LID - 10.1016/j.etap.2019.03.005 [doi]
AB  - beta-zearalenol (beta-zol) and HT-2 are mycotoxins which cause apoptosis and oxidative 
      stress in mammalian reproductive cells. Melatonin is an endogenous antioxidant 
      involved in apoptosis and oxidative stress-related activities. This study 
      investigated the effects of beta-zol and HT-2 on bovine ovarian granulosa cells 
      (BGCs), and how melatonin may counteract these effects. beta-zol and HT-2 inhibited 
      cell proliferation in a dose-dependent manner, and induced apoptosis of BGCs. 
      They also yielded upregulation of the apoptosis-related genes Bax/Bcl-2 and 
      Caspase3 and phosphorylation of p38MAPK. Increases in intracellular ROS were 
      observed along with higher levels of mRNA anti-oxidation markers SOD1, SOD2, and 
      CAT. SOD1, SOD2, malonaldehyde (MDA), and glutathione peroxidase (GSH-px) 
      activities increased, as did the levels of SOD1 and SOD2 proteins. All of these 
      effects were reduced or entirely attenuated in BGCs pre-treated with melatonin. 
      Our results demonstrate that melatonin has protective effects against 
      mycotoxin-induced apoptosis and oxidative stress in BGCs.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Yang, Fangxiao
AU  - Yang F
AD  - College of Animal Science and Technology, Nanjing Agricultural University, 
      Nanjing, China.
FAU - Li, Lian
AU  - Li L
AD  - College of Animal Science and Technology, Nanjing Agricultural University, 
      Nanjing, China.
FAU - Chen, Kunlin
AU  - Chen K
AD  - College of Animal Science and Technology, Nanjing Agricultural University, 
      Nanjing, China.
FAU - Li, Chengmin
AU  - Li C
AD  - College of Animal Science and Technology, Nanjing Agricultural University, 
      Nanjing, China.
FAU - Wang, Yiru
AU  - Wang Y
AD  - College of Animal Science and Technology, Nanjing Agricultural University, 
      Nanjing, China.
FAU - Wang, Genlin
AU  - Wang G
AD  - College of Animal Science and Technology, Nanjing Agricultural University, 
      Nanjing, China. Electronic address: glwang@njau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190307
PL  - Netherlands
TA  - Environ Toxicol Pharmacol
JT  - Environmental toxicology and pharmacology
JID - 9612020
RN  - 0 (Antioxidants)
RN  - 0 (zearalenol)
RN  - 76LO2L2V39 (Zeranol)
RN  - I3FL5NM3MO (T-2 Toxin)
RN  - JL5DK93RCL (Melatonin)
RN  - NC6C26RM46 (HT-2 toxin)
SB  - IM
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Apoptosis/drug effects
MH  - Cattle
MH  - Cells, Cultured
MH  - Female
MH  - Granulosa Cells/*drug effects/metabolism
MH  - Melatonin/*pharmacology
MH  - Oxidative Stress/drug effects
MH  - T-2 Toxin/*analogs & derivatives/toxicity
MH  - Zeranol/*analogs & derivatives/toxicity
OTO - NOTNLM
OT  - Alleviation effect
OT  - Apoptosis
OT  - HT-2 toxin
OT  - Melatonin
OT  - Oxidative stress
OT  - beta-zearalenol
EDAT- 2019/03/15 06:00
MHDA- 2019/07/12 06:00
CRDT- 2019/03/15 06:00
PHST- 2018/09/20 00:00 [received]
PHST- 2019/02/28 00:00 [revised]
PHST- 2019/03/05 00:00 [accepted]
PHST- 2019/03/15 06:00 [pubmed]
PHST- 2019/07/12 06:00 [medline]
PHST- 2019/03/15 06:00 [entrez]
AID - S1382-6689(18)30398-3 [pii]
AID - 10.1016/j.etap.2019.03.005 [doi]
PST - ppublish
SO  - Environ Toxicol Pharmacol. 2019 May;68:52-60. doi: 10.1016/j.etap.2019.03.005. 
      Epub 2019 Mar 7.